Macro- and microstructural descriptions of the zebrafish (Danio rerio) liver

• Autorzy: Batelli de Oliveira L. , Santos Leonardo A. , Mendes de Lima E.M.
• Języki publikacji: EN

Abstrakty

EN

The liver is an essential metabolically active organ, responsible for many vital life functions such as metabolism, synthesis, and storage. This study describes the macroscopic and microscopic zebrafish liver and its temporal changes during a period of 13 weeks. Forty-six adult zebrafish were used (46), of which 27 were females and were 19 males. Seven fish were euthanized initially for macroscopic evaluation and 3 fish were euthanized weekly for 13 weeks for microscopic evaluation. Routine histological technique was performed; the slides were stained with haematoxylin/eosin and periodic acid-Schiff. The volumetric (Vv(h)) and numerical (Nv(h)) densities of hepatocytes and vacuolation intensity were determined weekly. The liver was located in the coelomic cavity ventrally to the swim bladder, slightly brownish and divided into 3 hepatic lobes. Microscopically, it consisted of hepatocytes forming a muralium duplex around the sinusoids. The staining of the cytoplasm of hepatocytes was more eosinophilic and more basophilic in males and females, respectively. There was no formation of portal triads and phagocytic cells. The gallbladder had transitional epithelium, and the bile ducts had simple cuboidal epithelium. The volume and numerical density of hepatocytes during the 13 weeks was not statistically different, but these parameters displayed a positive correlation. The vacuolation was due to the presence of glycogen. The zebrafish liver has macro and micro structural particular features while, quantitatively, the volume and number of hepatocytes showed great capacity to respond to the physiological needs of the animal at the time. (Folia Morphol 2016; 75, 3: 382–387)

• Wydawca: -
• Czasopismo: Folia Morphologica
• Rocznik: 2016
• Tom: 75
• Numer: 3
• Opis fizyczny: p.382-387,fig.,ref.

Twórcy

autor

Batelli de Oliveira L.

• Laboratory of Veterinary Anatomy, Veterinary Hospital, University of Brasilia, Brasilia, Brazil

autor

Santos Leonardo A.

• Laboratory of Veterinary Anatomy, Veterinary Hospital, University of Brasilia, Brasilia, Brazil

autor

Mendes de Lima E.M.

• Laboratory of Veterinary Anatomy, Veterinary Hospital, University of Brasilia, ICC Ala Sul, Campus Darcy Ribeiro 4508, zip code 70760-701, Brasilia, Distrito Federal, Brazil

Bibliografia

• 1. Bombonato MTS, Rochel SS, Vincentini CA, Vicentini IBF (2007) Estudo morfológico do tecido hepático de Leporinus macrocephalus. Acta Sci Biol Sci, 29: 81–85.
• 2. Costa GM, Ortis RC, Lima MG, Casals JB Lima AR, Jr Kfoury JR (2012) Estrutura morfológica do fígado de tambaqui Colossoma macropomum (Cuvier, 1818). Pesq Vet Bras, 32: 947–950.
• 3. Gilbert MJ, Zerulla TC, Tierney KB (2014) Zebrafish (Danio rerio) as a model for the study of aging and exercise: physical ability and trainability decrease with age. Exp Gerontol, 50: 106–113.
• 4. Gupta T, Mullins MC (2010) Dissection of organs from the adult zebrafish. JoVE, 37: e.1717.
• 5. Fredello JP, Raqbi A, Mattei X, Viale D, Marchand B (2001) Quantification of macrophage aggregates in the liver of Mugil cephalus. J Submicr Cytol Pathol, 33: 473–473.
• 6. Li X, Elwell MR, Ryan AM, Ochoa R (2003) Morphogenesis of postmortem hepatocyte vacuolation and liver weight increases in Sprague-Dawley rats. J Toxicol Pathol, 31: 682–688.
• 7. Menke AL, Spitsbergen JM, Wolterbeek AP, Woutersen RA (2011) Normal anatomy and histology of the adult zebrafish. Toxicol Pathol, 39: 759–775.
• 8. Pankhurst NW, Munday PL (2011) Effects of climate change on fish reproduction and early life history stages. Mar Freshwater Res, 62: 1015–1026.
• 9. Rocha E, Rocha MJ, Galante MH, Silva MW, Monteiro RAF (2009) The hepatocytes of the brown trout (Salmo trutta fario): a stereological study of their number and size along the breeding cycle. Ichthyol Res, 56: 43–54.
• 10. Rocha E, Rocha MJ, Lobo-da-cunha A, Galante MH, Monteiro RA (2010) The hepatocytes of the brown trout (Salmo trutta fario): a stereological study of some cytoplasmic components with the breeding cycle. Microsc Res Tech, 73: 766–778.
• 11. Vicentini CA, Franceschini-Vicentini IB, Bombonato MTS, Bertolucci B, Lima SG, Santos AS (2005) Morphological study of the liver in the teleost Oreochromis niloticus. Int J Morphol, 23: 211–216.

Identyfikatory

DOI

10.5603/FM.a2016.0009