Czasopismo
Tytuł artykułu
Warianty tytułu
Języki publikacji
Abstrakty
Ginkgo suspension cells were used to investigate the mechanism that governs the shift between primary and secondary metabolism under NaCl elicitation. The production of three flavonol glycosides, chlorophyll fluorescence, ion content, the antioxidant system, and the cellular ultrastructure in the presence of NaCl doses from 5 to 175 mM were examined. At low salt doses (5–50 mM), cell growth and flavonol glycosides accumulation were stimulated without damaging cell structure or inducing oxidative stress by maintaining high K⁺ and chlorophyll content. At moderate salt doses (75–125 mM), the cells could withstand the salt stress without an impact on survival by changing internal cellular structure, maintaining high levels of K⁺ and Ca²⁺ and increasing anti-oxidative enzyme activities rather than flavonol glycosides to counteract the inhibition of the photosystem II, the accumulation of Na⁺ and hydrogen peroxide (H₂O₂) in the cells. This allowed cells to divert their metabolism from growth to defense-related pathways and tolerate NaCl stress. At higher salinity (150–175 mM), the cellular structure was damaged, and the high Na⁺ and low K⁺ content led to osmotic stress, and therefore, the stimulation of peroxidase (POD) and catalase (CAT) was not enough to cope with high H₂O₂ accumulation. The high production of flavonol glycosides may be a response of elicitation stimulation to serious damage at 175 mM NaCl. In conclusion, the use of 175 mM NaCl may be desirable for the induction of flavonol glycoside production in Ginkgo suspension cells.
Słowa kluczowe
Wydawca
Czasopismo
Rocznik
Tom
Numer
Opis fizyczny
p.3173-3187,fig.,ref.
Twórcy
autor
- College of Biology and Environment, Nanjing Forestry University, Nanjing, 210037, Jiangsu, China
autor
- College of Biology and Environment, Nanjing Forestry University, Nanjing, 210037, Jiangsu, China
autor
- College of Biology and Environment, Nanjing Forestry University, Nanjing, 210037, Jiangsu, China
autor
- College of Biology and Environment, Nanjing Forestry University, Nanjing, 210037, Jiangsu, China
autor
- College of Biology and Environment, Nanjing Forestry University, Nanjing, 210037, Jiangsu, China
autor
- College of Biology and Environment, Nanjing Forestry University, Nanjing, 210037, Jiangsu, China
autor
- College of Biology and Environment, Nanjing Forestry University, Nanjing, 210037, Jiangsu, China
autor
- College of Biology and Environment, Nanjing Forestry University, Nanjing, 210037, Jiangsu, China
Bibliografia
- Abogadallah GM (2010) Antioxidative defense under salt stress. Plant Signal Behav 5(4):369–374
- Abogadallah GM, Serag MM, Quick WP (2009) Fine and coarse regulation of reactive oxygen species in the salt tolerant mutants of barnyard grass and their wild-type parents under salt stress. Physiol Plant 138:60–73
- Abrol E, Vyas D, Koul S (2012) Metabolic shift from secondary metabolite production to induction of anti-oxidative enzymes during NaCl stress in Swertia chirata Buch.-Ham. Acta Physiol Plant 34:541–546
- Al-Khayri Jameel M (2002) Growth, proline accumulation, and ion content in sodium chloride-stressed callus of date palm. In Vitro Cell Dev Biol Plant 38:79–82
- Beyer WF, Fridovich I (1987) Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions. Anal Biochem 161:559–566
- Boughalleb F, Mounir DM, Tiba BB (2009) Photosystem II photochemistry and physiological parameters of three fodder shrubs, Nitraria retusa, Atriplex halimus and Medicago arborea under salt stress. Acta Physiol Plant 31:463–476
- Breia R, Vieira S, da Silva JM, Geros H, Cunha A (2013) Mapping grape berry photosynthesis by chlorophyll fluorescence imaging: the effect of saturating pulse intensity in different tissues. Photochem Photobiol 89:579–585
- Cai ZZ, Kastell A, Knorr D, Smetanska I (2012) Exudation: an expanding technique for continuous production and release of secondary metabolites from plant cell suspension and hairy root cultures. Plant Cell Rep 31:461–477
- Chen Y, Cao FL (2007) Leave source from callus induction and flavonoid content in callus from different tissues of five Ginkgo biloba L. cultivars. J Zhejiang For Coll 24(2):150–155 (in Chinese)
- Chen Y, Cao FL, Gan XH (2006) Protein analysis in Ginkgo calli with different colors by electrophoresis and electron microscopy. Acta Bot Boreal Occident Sin 26(11):2239–2243 (in Chinese)
- Cybulska-Heinrich AK, Mozaffarieh M, Flammer J (2012) Ginkgo biloba: an adjuvant therapy for progressive normal and high tension glaucoma. Mol Vis 18:390–402
- Davies BH (1976) Carotenoids. In: Good win TW chemistry and biochemistry of plant pigments (vol 2). Academic Press Inc, London, pp 38–165
- Demidchik V (2014) Mechanisms of oxidative stress in plants: From classical chemistry to cell biology. Environ Exp Bot. doi:10.1016/j.envexpbot.2014.06.021
- Dong JN, Wan GW, Liang ZS (2010) Accumulation of salicylic acid induced phenolic compounds and raised activities of secondary metabolic and antioxidative enzymes in Salvia miltiorrhiza cell culture. J Biotechnol 148:99–104
- Duncan DB (1955) Multiple range and multiple F test. Biometrics 11:1–42
- Ferreira AL, Lima-Costa ME (2008) Growth and ultrastructural characteristics of Citrus cells grown in medium containing NaCl. Biolgia Plantarum 52(1):129–132
- Fidalgo F, Santos A, Santos I, Salema R (2004) Effects of long-term salt stress on antioxidant defence systems, leaf water relations and chloroplast ultrastructure of potato plants. Ann Appl Biol 145:185–192
- Gauthami P, Subrahmanyam D, Padma V, Kiran TV, Rao YV, Rao PR, Voleti SR (2014) Variation in leaf photosynthetic response of rice genotypes to post-anthesis water deficit. Ind J Plant Physiol. doi:10.1007/s40502-014-0086-7
- Gill SS, Tuteja N (2010) Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem 48:909–930
- Guo ZG, Liu Y, Xing XH (2011) Enhanced catharanthine biosynthesis through regulation of cyclooxygenase in the cell suspension culture of Catharanthus roseus (L.). Process Biochem 46:783–787
- Hao GP, Du XH, Zhao FX, Shi RJ, Wang JM (2009) Role of nitric oxide in UV-B-induced activation of PAL and stimulation of flavonoid biosynthesis in Ginkgo biloba callus. Plant Cell Tissue Organ Cult 97:175–185
- Hao GP, Du XH, Zhao FX, Ji HW (2010) Fungal endophytes-induced abscisic acid is required for flavonoid accumulation in suspension cells of Ginkgo biloba. Biotechnol Lett 32:305–314
- Hao L, Zhao Y, Jin DD, Zhang L, Bi XH, Chen HX, Xu Q (2012) Salicylic acid-altering Arabidopsis mutants response to salt stress. Plant Soil 354:81–95
- Ho LC (2003) Interactions between root and shoot environmental factors on crop yield and quality. Acta Hortic 609:121–126
- Holden M (1965) Chlorophylls: chemistry and biochemistry of plant pigments. Academic Press, London, p 461
- James RA, Munns R, Caemmerer S, Trejo C, Miller C, Condon T (2006) Photosynthetic capacity is related to the cellular and subcellular partitioning of Na⁺, K⁺ and Cl⁻ in salt affected barley and durum wheat. Plant, Cell Environ 29:2185–2197
- Jeong GT, Park DH (2006) Enhanced secondary metabolite biosynthesis by elicitation in transformed plant root system. Appl Biochem Biotechnol 130(1):436–446
- Kang SM, Min JY, Kim YD, Kang YM, Park DJ, Jung HN, Kim SW, Choi MS (2006) Effects of methyl jasmonate and salicylic acid on the production of bilobalide and ginkgolides in cell cultures of Ginkgo biloba. In Vitro Cell Dev Biol Plant 42:44–49
- Kang SM, Min JY, Kim YD, Karigar CS, Kim SW, Goo GH, Choi MS (2009) Effect of biotic elicitors on the accumulation of bilobalide and ginkgolides in Ginkgo biloba cell cultures. J Biotechnol 139:84–88
- Kang SM, Min JY, Park DJ, Jeong MJ, Song HJ, Heo CM, Kim HG, Yang JK, Lee CH, Karigar CS, Choi MS (2010) Potassium chloride elicits enhancement of bilobalide and Ginkgolides production by Ginkgo biloba cell cultures. For Sci Technol 6:49–54
- Knorzer OC, Durner J, Boger P (1996) Alterations in the antioxidative system of suspension-cultured soybean cells (Glycine max) induced by oxidative stress. Physiol Plant 97:388–396
- Koukol J, Conn EE (1961) The metabolism of aromatic compounds in higher plants. Purification and properties of the L-phenylalanine de aminase of Hordeum vulagare. J Biol Chem 236:2692–2698
- Lattanzio V, Cardinali A, Ruta C, Fortunato IM, Lattanzio VMT, Linsalata V, Cicco N (2009) Relationship of secondary metabolism to growth in oregano (Origanum vulgare L.) shoot cultures under nutritional stress. Env Exp Bot 65:54–62
- Li CY, Liu B, Li CH, Zeng Q, Hao MZ, Han ZM, Zhu XG, Shen WJ (2013) Leaf morphology and ultrastructure responses to elevated O3 in transgenic Bt (cry1Ab/cry1Ac) rice and oxidative conventional rice under fully open-air field conditions. PLoS One 8(12):e82199
- MacDonald MJ, D’ Cunha GB (2007) A modern view of phenylalanine ammonialyase. Biochem Cell Biol 85:273–282
- Maria F, Stanisław W, Halina M, El Zbieta RS, Apolonia S, Jolanta BK (2012) Membrane permeability and micro-and macroelement accumulation in spring wheat cultivars during the shortterm effect of salinity and PEG induced water stresses. Acta Physiol Plant 34:985–995
- Mittal S, Kumari N, Sharma V (2012) Differential response of salt stress on Brassicajuncea: photosynthetic performance, pigment, proline, D1 and antioxidant enzymes. Plant Physiol Biochem 54:17–26
- Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–494
- Naidoo G, Rita S, Premila A (2008) Morphological and physiological responses of the halophyte, Odyssea paucinervis (Staph) (Poaceae), to salinity. Flora 203:437–447
- Niknam V, Meratan AA, Ghaffari SM (2011) The effect of salt stress on lipid peroxidation and antioxidative enzymes in callus of two Acanthophyllum species. In Vitro Cell Dev Biol Plant 47:297–308
- Noreen Z, Ashraf M (2009) Assessment of variation in antioxidative defense system in salt-treated pea (Pisum sativum) cultivars and its putative use as salinity tolerance markers. J Plant Physiol 116:1764–1774
- Osorio ML, Goncalves S, Coelho N, Osorio J, Romano A (2013) Morphological, physiological and oxidative stress markers during acclimatization and field transfer of micropropagated Tuberaria major plants. Plant Cell Tiss Organ Cult 115:85–97
- Queiros F, Rodrigues JA, Almeida JM, Almeida Domingos PF, Fidalgo F (2011) Differential responses of the antioxidant defence system and ultrastructure in a salt-adapted potato cell line. Plant Physiol Biochem 49:1410–1419
- Rao KVM, Sresty TVS (2000) Antioxidative parameters in the seedlings of pigeon pea (Cajanus cajan L. Millspaugh) in response to Zn and Ni stresses. Plant Sci 157:113–128
- Sabater-Jara AB, Souliman-Youssef S, Novo-Uzal E, Almagro L, Belchı-Navarro S, Pedreno MA (2013) Biotechnological approaches to enhance the biosynthesis of ginkgolides and bilobalide in Ginkgo biloba. Phytochem Rev 12:191–205
- Santamaria AR, Mulinacci N, Valletta A, Innocenti M, Pasqua G (2011) Effects of elicitors on the production of resveratrol and viniferins in cell cultures of Vitis vinifera L. cv Italia. J Agric Food Chem 59(17):9094–9101
- Sarkar RK, Mahata KR, Singh DP (2013) Differential responses of antioxidant system and photosynthetic characteristics in four rice cultivars differing in sensitivity to sodium chloride stress. Acta Physiol Plant 35:2915–2926
- Sekmen AH, Turkan I, Ozgecan TZ, Ceyda O, Dinc A (2012) Different antioxidant defense responses to salt stress during germination and vegetative stages of endemic halophyte Gypsophila oblanceolata Bark. Environ Exp Bot 77:63–76
- Shu S, Yuan LY, Guo SR, Sun J, Yuan YH (2013) Effects of exogenous spermine on chlorophyll fluorescence, antioxidant system and ultrastructure of chloroplasts in Cucumis sativus L. under salt stress. Plant Physiol Biochem 63:209–216
- Singh B, Kaur P, Gopich, Singh RD, Ahuja PS (2008) Biology and chemistry of Ginkgo biloba. Fitoterapia 79:401–418
- Solecka D, Kacperska A (2003) Phenylpropanoid deficiency affects the course of plant acclimation to cold. Physiol Plantarum 119:253–262
- Stahl W, Sies H (2003) Antioxidant activity of carotenoids. Mol Aspects Med 24:345–351
- Sun J, Li LS, Liu MQ, Wang MJ, Ding MQ, Deng SR, Lu CF, Zhou XY, Shen X, Zheng XJ, Chen SL (2010) Hydrogen peroxide and nitric oxide mediate K⁺/Na⁺ homeostasis and antioxidant defense in NaCl-stressed callus cells of two contrasting poplars. Plant Cell Tiss Organ Cult 103:205–215
- Sun LN, Su H, Zhu Y, Xu MJ (2012) Involvement of abscisic acid in ozone-induced puerarin production of Pueraria thomsnii Benth. suspension cell cultures. Plant Cell Rep 31:179–185
- Sykłowska-Baranek K, Pietrosiuk A, Naliwajski M, Kawiak A, Jeziorek M, Wyderska S, Lojkowska E, Chinou I (2012) Effect of L-phenylalanine on PAL activity and production of naphthoquinone pigments in suspension cultures of Arnebia euchroma (Royle) Johnst. In Vitro Cell Dev Biol Plant 48:555–564
- Tounekti T, Vadel AM, Onate M, Khemira H, Munné-Bosch S (2011) Salt-induced oxidative stress in rosemary plants: damage or protection? Environ Exp Bot 71:298–305
- Upton R (ed) (2003) Ginkgo leaf–Ginkgo leaf dry extract—Ginkgo biloba L.—standards of analysis, quality control, and therapeutics. American herbal pharmacopoeia, Scotts Valley
- Van Beek TA, Montoro P (2009) Chemical analysis and quality control of Ginkgo biloba leaves, extracts, and phytopharmaceuticals. J Chromatogr A 1216:2002–2032
- Wang GB, Cao FL, Zhang WX (2003) A study on salt tolerance of Ginkgo biloba varieties. Scientia silvae Sinicae 39(5):168–172 (in Chinese)
- Wang GB, Cao FL, Jing M, Hao MZ (2008) The effect of water stress on mesophyll cell ultrastructure of Ginkgo. J Nanjing For Univ (Nat Sci Ed) 32(5):65–70 (in Chinese)
- Wang YW, Sun LG, Xu JY, Zhang JJ, Wu M, Ma J, Chen GX (2014) Physiological and growth characteristics of Ginkgo biloba L. exposed to open field and shade enclosure during the reproductive stage. Acta Physiol. doi:10.1007/s11738-014-1638-1
- Yusuf NA, Annuar MSM, Khalid N (2013) Physical stress for over production of biomass and flavonoids in cell suspension cultures of Boesenbergia rotunda. Acta Physiol Plant 35:1713–1719
- Zhao X, Tan HJ, Liu YB, Li XR, Chen GX (2009) Effect of salt stress on growth and osmotic regulation in Thellungiella and Arabidopsis callus. Plant Cell Tiss Organ Cult 98:97–103
- Zhao B, Agblevor FA, Ritesh KC, Jelesko JG (2013) Enhanced production of the alkaloid nicotine in hairy root cultures of Nicotiana tabacum L. Plant Cell Tiss Organ Cult 113:121–129
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-5905317b-87ae-4962-857a-97037b34df41