Effects of maternal thyroid hormone deficiency on differentiation of mesenchymal stem cells in CSF‑exposed neonatal Wistar rats

• Autorzy: Mafikandi V. , Roodbari N.H. , Nabiuni M. , Yaghmaei P.
• Języki publikacji: EN

Abstrakty

EN

Cerebrospinal fluid (CSF) contains growth and neurotrophic factors which regulate proliferation, differentiation, and neurogenesis. Thyroid hormones play a crucial role in the development of the nervous system and hypothyroidism during development of embryos leads to defects in the nervous system. This study aimed to survey the effects of rat neonatal CSF collected from induced hypothyroid mothers on differentiation of bone marrow mesenchymal stem cells (BM‑MSCs). We hypothesized that hypothyroidism affected levels of growth factor in CSF. To induce hypothyroidism, pregnant Wistar rats received methimazole at the third day of gestation. BM‑MSCs were obtained from rat femurs and tibias and cultured in medium. CSF was collected from the cisterna magna of newborn rats, and cells were subsequently exposed to CSF with concentrations of 5,7, and 10 /100 (v/v) for 72 h. MTT (3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide) assay and real time polymerase chain reaction (RT‑PCR) were used to quantify the cell viability and analyze the expression of neural markers, respectively. Our morphological studies showed that treatment with hypothyroidism CSF (HTH‑CSF) resulted in a significant decrease in neurite growth and proliferation as compared to normal CSF (N‑CSF). RT‑PCR analysis also showed a significant decrease in expression of neural markers (i.e., Nestin, Neurod‑1, NeuN) in cells treated with HTH‑CSF as compared with the N‑CSF group. The most effective concentration of CSF for BM‑MSC differentiation was 5% (V/V). Our results showed a significant decrease in differentiation of BM‑MSCs in the presence of neonatal CSF of hypothyroid mothers compared with neonatal CSF of healthy mothers. Thus, thyroid hormones are essential in neural development and hypothyroid defects can affect development of the neonatal brain.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2019
• Tom: 79
• Numer: 3
• Opis fizyczny: p.270275,fig.,ref.

Twórcy

autor

Mafikandi V.

• Science and Research Branch, Department of Biology, Islamic Azad University, Tehran, Iran

autor

Roodbari N.H.

• Science and Research Branch, Department of Biology, Islamic Azad University, Tehran, Iran

autor

Nabiuni M.

• Faculty of Biological Sciences, Kharazmi University, Tehran, Iran

autor

Yaghmaei P.

• Science and Research Branch, Department of Biology, Islamic Azad University, Tehran, Iran

Bibliografia

• Ahmed OM, El‑Gareib AW, El‑bakry AM, Abd El‑Tawab SM, Ahmed RG (2008) Thyroid hormones states and brain development interactions. Int J Dev Neurosci 2: 147–209.
• Ahmed RG (2018) Interactions between thyroid and growth factors during development. ARC J Diabet Endocrinol 4: 1–4.
• Bernal J (2015) Thyroid hormones in brain development and function. Comprehensive free online endocrinology book. www.endotext.org . Chatonnet F, Picou F, Fauquier T and Flamant F (2011) Thyroid hormone action in cerebellum and cerebral cortex development. J Thyroid Res 2011: 145762.
• Fisher DA, Hoath S, Lakshmanan J (1982) the thyroid hormone effects on growth and development may be mediated by growth factors. Endocri‑ nol Exp 16: 259–271.
• Gusel’nikova VV, Korzhevskiy D E (2015) NeuN as a neuronal nuclear anti‑ gen and neuron differentiation marker. Acta Naturae 2: 42–47.
• Liu J, Ji X, Li Z, Zheng H, Zheng W, Jia J, Shen H, Zhang Q, An J (2015) Nes‑ tin overexpression promotes the embryonic development of heart and brain through the regulation of cell proliferation. Brain Res 1610: 1–11.
• Miranda A, Sousa N (2018) Maternal hormonal milieu influence on fetal brain development. Brain Behav 8: e00920. Mohan V, Sinha RA, Pathak A, Rastogi L, Kumar P, Pal A, Godbole MM (2012) Maternal thyroid hormone deficiency affects the fetal neocorticogene‑ sis by reducing the proliferating pool, rate of neurogenesis and indirect neurogenesis. Exp Neurol 237: 477–488.
• Mussa GC, Mussa F, Bretto R, Zambelli MC, Silvestro L (2001) Influence of thyroid in nervous system growth. Minerva Pediatr 53: 325–353.
• Nabiuni M, Shokohi R, Moghaddam P (2015) CSF protein contents and their roles in brain development. Zahedan J Res Med Sci 17: e1042.
• Nabiuni M, Rasouli J, Parivar K, Kochesfehani HM, Irian S, Miyan J (2012) In vitro effects of fetal rat cerebrospinal fluid on viability and neuronal differentiation of PC12 cells. Fluids Barriers CNS 9: 8
• Nava MM, Raimondi MT, and Pietrabissa R (2012) Controlling self‑renewal and differentiation of stem Cells via mechanical cues. Hindawi publish‑ ing corporation J Biomed Biotechnol 2012: 797410.
• Pataskar A, Jung J, Smialowski P, Noack F, Calegari F, Straub T, Tiwari VK (2016) NeuroD1 reprograms chromatin and transcription factor land‑ scapes to induce the neuronal program. EMBO J 35: 24–45.
• Sahay RK and Nagesh VS (2012) Hypothyroidism in pregnancy. Indian J En‑ docr Metab 16: 364–370.
• Shafiee SM, Vafaei AA, Rashidy‑Pour A (2016) Effects of maternal hypothy‑ roidism during pregnancy on learning, memory and hippocampal BDNF in rat pups: Beneficial effects of exercise. Neuroscience 329: 151–161.
• Shokohi R, Nabiuni M, Irian S, Miyan JA (2018) In vitro effects of Wistar rat prenatal and postnatal cerebrospinal fluid on neural differentiation and proliferation of mesenchymal stromal cells derived from bone marrow. Cell J 4: 537–544.
• Shokohi R, Nabiuni M, Moghaddam P, Irian S, Miyan JA (2017) Fetal cere‑ brospinal fluid promotes proliferation and neural differentiation of stro‑ mal mesenchymal stem cells derived from bone marrow. Braz Arch Biol Technol 60: doi.org/10.1590/1678–4324.
• Yari S, Parivar K, Nabiuni  M, Keramatipour MY (2012) Effect of e‑CSF on morphological changes of neurosphere derived astrocytes. J Am Sci 8: 1109–1113.
• Yoshii A, Constantine‑Paton  M (2010) Postsynaptic BDNF‑Trk B signal‑ ing in synapse maturation, plasticity and disease. Dev Neurobiol 70: 304–322.
• Zheng Y, Huang C, Liu F, Lin H, Yang X, Zhang Z (2017) Comparison of the neuronal differentiation abilities of bone marrow‑derived and adipose tissue‑derived mesenchymal stem cells. Mol Med Rep 4: 3877–3886.

Identyfikatory

DOI

10.21307/ane‑2019‑025