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Abstrakty
Linum album has been shown to accumulate some lignans with antiviral and anticancer properties such as podophyllotoxin (PTOX) and 6-methoxy podophyllotoxin (MPTOX). In this research, we examined the effects of fungal elicitors on the production of lignans in L. album hairy root cultures. The biosynthesis of lignans was differentially affected by fungal elicitors. Fusarium graminearum extract induced the highest increase of PTOX, 190 μg g⁻¹ dry weight (DW), and lariciresinol, 260 μg g⁻¹ DW, which was two-fold and three-fold greater than the untreated control, respectively, while Trichoderma viride extract enhanced the accumulation of MPTOX, instead of PTOX, up to 160 μg g⁻¹ DW, which was 2.4-fold greater than the control. The enhancing effects of fungal elicitors on lignans production was correlated with the increased expression of some key genes involved in the biosynthesis of these compounds, phenylalanine ammonia-lyase, cinnamoyl-CoA reductase, cinnamyl-alcohol dehydrogenase and pinoresinol-lariciresinol reductase.
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Opis fizyczny
p.3325-3331,fig.,ref.
Twórcy
autor
- Department of Biology, Faculty of Basic Sciences, University of Zabol, Zabol, Iran
autor
- Department of Plant Biology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
autor
- Department of Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran
autor
- Suntory Foundation for Life Sciences, Bioorganic Research Institute, Osaka, Japan
autor
- Suntory Foundation for Life Sciences, Bioorganic Research Institute, Osaka, Japan
Bibliografia
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- Chashmi NA, Sharifi M, Yousefzadi M, Behmanesh M, Rezadoost H, Cardillo A, Palazon J (2012) Analysis of 6-methoxy podophyllotoxin and podophyllotoxin in hairy root cultures of Linum album Kotschy ex Boiss. Med Chem Res 22:745–752
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Bibliografia
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