Male-specific repeats in wild Bovidae

• Autorzy: Cabelova K. , Kubickova, S. , Cernohorska H. , Rubes J.
• Języki publikacji: EN

Abstrakty

EN

In this study, we investigated repetitive sequences localized on Y chromosomes. Repetitive DNA sequences represent a substantial part of the eukaryotic genome and, among them, a large portion comprises sequences repeated in tandem. Efficient and rapid isolation of repeat units is possible due to a laser microdissection technique used for Y chromosome separation, followed by polymerase chain reaction (PCR), cloning, and sequence analysis. We applied the derived repeat units to members of nine tribes within the Bovidae. Apart from the Y chromosomes of Bos taurus and Bubalus bubalis, where we used known sequences of repetition, the derived sequences were used as probes for fluorescent in situ cross-hybridization to members of the nine tribes of the Bovidae. We investigated the distribution of repeat units within the tribes and their localization on the Y chromosome. Sharing of sequence variants would indicate common descent, while the rapid horizontal evolution should allow discrimination between closely related species or subspecies.

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 2012
• Tom: 53
• Numer: 4
• Opis fizyczny: p.423-433,fig.,ref.

Twórcy

autor

Cabelova K.

• Department of Genetics and Reproduction, Veterinary Research Institute, Hudcova 70, 621 00, Brno, Czech Republic

autor

Kubickova, S.

autor

Cernohorska H.

autor

Rubes J.

Uwagi

PL

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