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Liczba wyników
2008 | 52 | 4 |
Tytuł artykułu

Immune responses induced in hens after oral administration of bovine serum albumin in combination with an extract of Uncaria tomentosa

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
A dry extract from the bark of Uncaria tomentosa was evaluated as a potential immunomodulator in various formulations. Hens were immunised three times orally with a bovine serum albumin (BSA) supplemented with 7, 70, and 280 mg of the dry extract of the bark. Specific IgA antibodies in saliva and IgY antibodies in yolk were examined by ELISA. It was found that the birds inoculated with BSA in addition to 280 mg of Uncaria tomentosa bark extract had a higher amount of BSA-specific IgA antibodies in the saliva and BSA-specific IgY antibodies in yolk, than hens that received lower doses of the extract. Furthermore, the hens immunised with BSA alone had significantly lower immune responses to BSA than hens immunised with BSA supplemented with the bark extract. These results suggest that dry extract from bark of Uncaria tomentosa is useful as an oral adjuvant or immunomodulator for hens.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
52
Numer
4
Opis fizyczny
p.667-670,fig.,ref.
Twórcy
  • Laboratory of Biomodels, Institute of Immunology, Vilnius University, LT-08409 Vilnius, Lithuania
autor
  • Laboratory of Biomodels, Institute of Immunology, Vilnius University, LT-08409 Vilnius, Lithuania
Bibliografia
  • 1. Aquino R., de Simone F., Pizza C., Conti C., Stein M.L.: Plant metabolites. Structure and in vitro anti-viral activity of quinovic acid glycosides from Uncaria tomentosa and Guettarda platypoda. J Nat Prod 1989, 52, 679-689.
  • 2. Aquino R., de Feo V., de Simone F., Pizza C., Cirino G.: Plant metabolites. New components and anti-inflammatory activity of Uncaria tomentosa. J Nat Plant 1991, 54, 453-445.
  • 3. Bižanov G., Tamošiūnas V.: Immune responses induced in mice after intragastral administration with Sendai virus in combination with extract of Uncaria tomentosa. Scand J Lab Anim Sci 2005, 32, 201-207.
  • 4. Bollen L.S., Crowley G., Stodulski G., Hau J.: Antibody production in rabbits and chickens immunized with human IgG. A comparison of titre and avidity development in rabbit serum, chicken serum and egg yolk using three different adjuvants. J Immunol Methods 1995, 191, 113-120.
  • 5. Desmarchelier C., Mongelli E., Coussio J., Ciccia G.: Evaluation of the in vitro antioxidant activity in extracts of Uncaria tomentosa. Phytother Res 1997, 11, 254-256.
  • 6. Hurn A.L., Chantler S.M.: Production of reagent antibodies. In: Methods in Enzymology, edited by H.Van Vanakis, J.J.Langone, New York, Academic Press, 1980, (part A), pp. 104-142.
  • 7. Keplinger K., Laus G., Wurm M., Dierich M.P., Teppner H.: Uncaria tomentosa (Willd.) DC. - Ethnomedicinal use and new pharmacological, toxicological and botanical results. J Ethnopharmacol 1999, 64, 23-34.
  • 8. Lemaire I., Assinewe V., Cano P., Awang D., Arnason J.T.: Stimulation of interleukin-1 and -6 production in alveolar macrophages by the neotropical liana, Uncaria tomentosa (Uña de Gato). J Ethnopharmacol 1999, 64, 109-115.
  • 9. McGhee J.R., Mestecky M.T., Dertzbaugh J.H., Eldrige M., Hirasawa M., Kijono H.: The mucosal immune system: from fundamental conceptions to vaccine development. Vaccine 1992, 10, 75-88.
  • 10. Reinhard K.H.: Uncaria tomentosa (Willd) DC: cat's claw, uña de cato, or saventaro. J Altem Complement Med 1999, 5, 143-151.
  • 11. Rizzi R., Re F., Bianchi A., de Feo V., de Simone F., Bianchi L., Stivala L.A.: Mutagenic and antimutagenic activities of Uncaria tomentosa and its extracts. J Ethnopharmacol 1993, 38, 63-77.
  • 12. Rivera E., Daggfeldt A., Hu S.: Ginseng extract in aluminium hydroxide adjuvanted vaccines improves the antibody response of pigs to porcine parvovirus and Erysipelothrix rhusiopathiae. Vet Immunol Immunopathol 2003, 91, 19-27.
  • 13. Svendsen L., Crowley A., Ostergaard L.H., Stodulski G., Hau J.: Development and comparison of purification strategies for chicken antibodies from egg yolk. Lab Anim Sci 1995, 45, 89-93.
  • 14. Walker R.I.: New strategies for using mucosal vaccination to achieve more effective immunization. Vaccine 1994, 12, 387-400.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
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