IS6110-based differentiation of Mycobacterium tuberculosis strains

• Autorzy: Zaczek A. , Ziolkiewicz M. , Wojtasik A. , Dziadek J. , Sajduda A.
• Języki publikacji: EN

Abstrakty

EN

In this study, 62 Mycobacterium tuberculosis strains were characterized by fast ligation-mediated PCR (FLiP) and, previously performed, IS6110 restriction fragment length polymorphism (RFLP). FLiP proved a reproducible and specific method for differentiation between M. tuberculosis strains. The discriminatory power of FLiP was close to that of the reference IS6110 RFLP suggesting its usefulness in studying the genetic diversity of M. tuberculosis strains.

Słowa kluczowe

EN

IS6110 gene; differentiation; Mycobacterium tuberculosis; restriction fragment length polymorphism; tuberculosis; mortality; morbidity; polymerase chain reaction; bacterial strain

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2013
• Tom: 62
• Numer: 2
• Opis fizyczny: p.201-204,fig.,ref.

Twórcy

autor

Zaczek A.

• Department of Biochemistry and Cell Biology, Faculty of Biology and Agriculture, University of Rzeszow, Cwiklinskiej 2, 35-601 Rzeszow, Poland

autor

Ziolkiewicz M.

• Institute of Medical Biology, Polish Academy of Sciences, Lodowa 106, 93-232 Lodz, Poland

autor

Wojtasik A.

• Institute of Medical Biology, Polish Academy of Sciences, Lodowa 106, 93-232 Lodz, Poland

autor

Dziadek J.

• Department of Biochemistry and Cell Biology, Faculty of Biology and Agriculture, University of Rzeszow, Cwiklinskiej 2, 35-601 Rzeszow, Poland
• Institute of Medical Biology, Polish Academy of Sciences, Lodowa 106, 93-232 Lodz, Poland

autor

Sajduda A.

• Department of Genetics of Microorganisms, Faculty of Biology and Environmental Protection, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland

Bibliografia

• Behr M.A. and S. Mostowy. 2007. Molecular tools for typing and branding the tubercle bacillus. Curr. Mol. Med. 7: 309–317.
• Cave M.D., M. Murray and E. Nardell. 2005. Molecular epidemiology of Mycobacterium tuberculosis, pp. 33–46. In: Cole S.T., K.D. Eisenach, D.N. McMurray and W.R. Jacobs, Jr. (eds.). Tuberculosis and the Tubercle Bacillus. ASM Press, Washington, D.C.
• Dela A., A. Sajduda, I. Pawłowska and J. Dziadek. 2006. Molecular characterization of Mycobacterium tuberculosis isolates from Łódź, Poland: analysis by IS6110 restriction fragment length polymorphism and double-repetitive-element PCR. J. Infect. 52: 346–353.
• Haas W.H., W.R. Butler, C.L. Woodley and J.T. Crawford. 1993. Mixed-linker polymerase chain reaction: a new method for rapid fingerprinting of isolates of the Mycobacterium tuberculosis complex. J. Clin. Microbiol. 31: 1293–1298.
• Hunter P.R. and M.A. Gaston. 1988. Numerical index of the discriminatory ability of typing systems: an application of Simpson’s index of diversity. J. Clin. Microbiol. 26: 2465–2466.
• Krawczyk M., A. Brzostek, A. Gorna, K. Knapska, M. Ziolkiewicz, A. Wojtasik and J. Dziadek. 2011. Epidemiological analysis of Mycobacterium tuberculosis strains isolated in Lodz, Poland. Int. J. Tuberc. Lung Dis. 15: 1252–1258.
• Kremer K., C. Arnold, A. Cataldi, M.C. Gutiérrez, W.H. Haas, S. Panaiotov, R.A. Skuce, P. Supply, A.G.M. van der Zanden and D. van Soolingen. 2005. Discriminatory power and reproducibility of novel DNA typing methods for Mycobacterium tuberculosis complex strains. J. Clin. Microbiol. 43: 5628–5638.
• Mathema B., N.E. Kurepina, P.J. Bifani and B.N. Kreiswirth. 2006. Molecular epidemiology of tuberculosis: current insights. Clin. Microbiol. Rev. 19: 658–685.
• Reisig F., K. Kremer, B. Amthor, D. van Soolingen and W.H. Haas. 2005. Fast ligation-mediated PCR, a fast and reliable method for IS6110-based typing of Mycobacterium tuberculosis complex. J. Clin. Microbiol. 43: 5622–5627.
• Rozo-Anaya J.C. and W. Ribón. 2010. Molecular tools for Mycobacterium tuberculosis genotyping. Rev. salud pública 12: 510–521.
• van Embden J.D., M.D. Cave, J.T. Crawford, B. Gicquel, P. Hermans, R. McAdam, T. Shinnick and P.M. Small. 1993. Strain identification of Mycobacterium tuberculosis by DNA fingerprinting: recommendations for a standardized methodology. J. Clin. Microbiol. 31: 406–409.
• World Health Organization. 2010. Global tuberculosis control: WHO Report 2010. WHO Press, Geneva.