Differentiation of reference and industrial baker’s yeast strains by classical biochemical tests and PCR-RAPD method

• Autorzy: Lipinska E. , Gniewosz M. , Juchimiuk M.
• Języki publikacji: EN

Abstrakty

EN

The purpose of the study was to apply traditional and genetic methods for discrimination of baker’s yeasts strains. The analysed strains were subjected to preliminary morphological characterization by microscopic and physiological observations by means of API 20 C AUX tests. Their DNA analyses were then carried out using the RAPD technique with the following primers: AB1-12 (5’CCTTGACGCA 3’), primer 21 (5’GCTCGTCGCT 3’), and primer 24 (5’GCGTGACTTG 3’). The analysis of assimilation and genetics profiles showed both similarity and differences between the investigated strains. As a result, the following groups of strains of baker’s yeast have been discriminated: I group: 2200, 2200 – 3PJ, 2200-4PJ; II group: I1, I2, AFr; III group: IRHS, 102, D1; and IV group: HH. The strain ATCC 2366 showed different profiles in both methods regarding to the investigated strains of bakers yeasts, which proved the usefulness of these methods in strains discrimination within the S. cerevisiae species.

• Wydawca: -
• Czasopismo: Polish Journal of Food and Nutrition Sciences
• Rocznik: 2009
• Tom: 59
• Numer: 1
• Opis fizyczny: p.73-79,fig.,ref.

Twórcy

autor

Lipinska E.

• Department of Biotechnology, Microbiology and Food Evaluation, Warsaw University of Life Sciences, Nowoursynowska 159c, 02-767 Warsaw, Poland

autor

Gniewosz M.

autor

Juchimiuk M.

Bibliografia

• 1. Arias C.R., Burns J.K., Friedrich L.M., Goodrich R.M., Parish M.E., Yeast species associated with orange juice: Evaluation of different identification methods. Appl. Environ. Microbiol., 2002, 4, 1955–1961.
• 2. Baleiras-Couto M.M., van der Vossen J.M.B.M., Hofstra H., Huis in‘t Veld J.H.J., RAPD analysis: a rapid technique for differentiation of spoilage yeasts. Int. J. Food Microbiol., 1994, 24, 249–260.
• 3. Baleiras-Couto M.M., Eijsma B., Hofstra H., Huis in’t Veld J.H.J., van der Vossen J.M.B.M., Evaluation of molecular typing techniques to assign genetic diversity among Saccharomyces cerevisiae strains. Appl. Environ. Microbiol., 1996, 62, 41–46.
• 4. Barszczewski W., Robak M., Differentiation of contaminating yeasts in brewery by PCR-based techniques. Food Microbiol., 2004, 21, 227–231.
• 5. Barszczewski W., Robak M., PCR – based differantiation and homology of brewing and type strains o the genus Saccharomyces. J. Inst. Brewing, 2006, 112, 165–172.
• 6. Echeverrigaray S., Paese-Toresan S., Carrau J., RAPD marker polymorphism among commercial winery yeast strains. World J. Microb. Biot., 2000, 16, 143–146.
• 7. Fadda M., Mossa V., Pisano M., Deplano M., Cosentino S., Occurrence and characterization of yeasts isolated from artisanal Fiore Sardo cheese. Int. J. Food Microbiol., 2004, 95, 51–59.
• 8. Foschino R., Gallina S., Andrighetto Ch., Rossetti L., Galli A., Comparison of cultural methods for the identification and molecular investigation of yeasts from sourdoughs for Italian sweet baked products. FEMS Yeast Res., 2004, 4, 609–618.
• 9. Hayford A., Jespersen L., Characterization of Saccharomyces cerevisiae strains from spontaneously fermented maize dough by profiles of assimilation, chromosome polymorphism, PCR and Mal genotyping. J. Appl. Microbiol., 1999, 86, 284–294.
• 10. Josepa S., Guillamon J. M., Cano J., PCR differentiation of Saccharomyces cerevisiae from Saccharomyces bayanus/Saccharomyces pastorianus using specific primers. FEMS Microbiol. Lett., 2000, 193, 255–259.
• 11. Penner G., Bush A., Wise R., Kim W., Domier L., Kasha K., Laroche A., Scoles G., Molnar S., Fedak G., Reproducibility of random amplified polymorphic DNA (RAPD) analysis among laboratories. PCR Methods and Applications, 1993, 2, 341–345.
• 12. Robak M., Baranowska K., Barszczewski W., Wojtatowicz M., RAPD as a tool for differentiation and identification of yeast. Biotechnologia, 2005, 4, 142–155, (in Polish).
• 13. Succi M., Reale A., Andrighetto C., Lombardi A., Sorrentino E., Coppola R., Presence of yeasts in southern Italian sourdoughs from Triticum aestivum flour. FEMS Microbiol. Lett., 2003, 225, 143–148.
• 14. Tornai-Lehoczki J., Dlauchy D., Delimination of brewing yeast strains using different molecular techniques. Int. J. Food Microbiol., 2000, 62, 37–45.
• 15. Török T., King A. Jr, Comparative study on the identification of food-borne yeasts. Appl. Environ. Microbiol., 1991, 4, 1207–1212.
• 16. Tyler K.D., Wang G., Tyler S.D., Johnson W.M., Factors affecting reliability and reproducibility of amplification-based DNA fingerprinting of representative bacterial pathogens. J. Clin. Microbiol., 1997, 35, 339–346.
• 17. Van de Peer Y., De Wachter R., TREECON for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Computer Applications in The Biosciences, 1994, 10, 569–570.
• 18. Vaughan-Martini A., Martini A., Description of teleomorphic ascomycetous genera and species; Saccharomyces: 1998, in: The Yeasts. A Taxonomic Study, IVth ed. (eds. C. Kurtzman, J. Fell). Elsevier Science B.V., Amsterdam, The Netherlands, pp. 358–363.
• 19. Vernocchi P., Valmorri S., Gatto V., Torriani S., Gianotti A., Suzzi G., Guerzoni M., Gardini F., A survey on yeast microbiota associated with an Italian traditional sweet-leavened baked good fermentation. Food Res. Int., 2004, 37, 469–476.
• 20. Williams J., Kubelik A., Livak K., Rafalski A., Tingey S., DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl. Acids Res., 1990, 22, 6531–6535.
• 21. Xufre A., Simões F., Gírio F., Clemente A., Amaral-Collaço T., Use of RAPD analysis for differentiation among six enological Saccharomyces spp. strains. Food Technol. Biotechnol., 2000, 1, 53–58.

Uwagi

PL

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