Enhanced salinity tolerance and improved yield properties in Bangladeshi rice Binnatoa through Agrobacterium-mediated transformation of PgNHX1 from Pennisetum glaucum

• Autorzy: Islam S.M.T. , Tammi R. S. , Singla-Pareek S. L. , Seraj Z. I.
• Języki publikacji: EN

Abstrakty

EN

Rice yield is severely affected by high-salt concentration in the vicinity of the plant. In an effort to engineer rice for improved salt tolerance Agrobacterium-mediated transformation of rice cv. Binnatoa was accomplished with the Pennisetum glaucum vacuolar Na⁺/H⁺ antiporter gene (PgNHX1) under the constitutive CaMV35S promoter. For the molecular analysis of putative transgenic plants, PCR and RT-PCR were performed. Transgenic rice plants expressing PgNHX1 showed better physiological status and completed their life cycle by setting flowers and seeds in salt stress, while wild-type plants exhibited rapid chlorosis and growth inhibition. Moreover, transgenic rice plants produced higher grain yields than wild-type plants under salt stress. Assessment of the salinity tolerance of the transgenic plants at seedling and reproductive stages demonstrated the potential of PgNHX1 for imparting enhanced salt tolerance capabilities and improved yield.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2010
• Tom: 32
• Numer: 4
• Opis fizyczny: p.657-663,fig.,ref.

Twórcy

autor

Islam S.M.T.

• Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, Bangladesh

autor

Tammi R. S.

• Department of Biochemistry and Molecular Biology, Jahangirnagar University, Savar, Bangladesh

autor

Singla-Pareek S. L.

• International Centre for Genetic Engineering and Biotechnology, New Delhi, India

autor

Seraj Z. I.

• Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, Bangladesh

Bibliografia

• Akbar M, Ponnamperuma FN (1980) Saline soil of south and southeast asia as potential rice lands. In: Rice research strategies for the future, IRRI, Los Banos, Laguna, Philippines, pp 256–281
• Amtmann A, Sanders D (1999) Mechanisms of Na⁺ uptake by plant cells. Adv Bot Res 29:75–112
• Apse MP, Aharon GS, Snedden WA, Blumwald E (1999) Salt tolerance conferred by overexpression of a vacuole Na⁺/H⁺ antiport in Arabidopsis. Science 285:1256–1258
• Ballesteros E, Blumwald E, Donaire JP, Belver A (1997) Na⁺/H⁺ antiport activity on tonoplast vesicles isolated from sunflower induced by NaCl stress. Physiol Plant 99:328–334
• Barkla B, Charuk JHM, Cragoe EJ, Blumwald E (1990) Photolabeling of tonoplast from sugar beet cell suspensions by [3H]5-(N-methyl-N-isobotyl)-amiloride, and inhibitor of the vacuolar Na⁺/H⁺ antiport. Plant Physiol 93:924–930
• Blumwald E, Poole RJ (1985) Na⁺/H⁺ antiport in isolated tonoplast vesicles from storage tissue of Beta vulgaris. Plant Physiol 78:163–167
• Blumwald E, Aharon GS, Apes MP (2000) Sodium transport in plant cells. Biochem Biophys Acta 1465:140–151
• Fukuda A, Nakamura A, Tanaka Y (1999) Molecular cloning and expression of the Na⁺/H⁺ exchanger gene in Oryza sativa. Biochim Biophys Acta 1446:149–155
• Fukuda A, Nakamura A, Tagiri A, Tanaka H, Miyao A, Hirochika H, Tanaka Y (2004) Function, intracellular localization and the importance in salt tolerance of a vacuolar Na⁺/H⁺ antiporter from rice. Plant Cell Physiol 45:146–159
• Gao J-P, Chao D-Y, Lin H-X (2007) Understanding abiotic stress tolerance mechanisms: recent studies on stress response in rice. J Integr Plant Biol 49(6):742–750
• Gaxiola RA, Rao R, Sherman A, Grisafi P, Alper SL, Fink GR (1999) The Arabidopsis thaliana transporters, AtNHX1 and Avp1, can function in cation detoxification in yeast. Proc Natl Acad Sci USA 96:1480–1485
• Glenn E, Brown JJ, Blumwald E (1999) Salt-tolerant mechanisms and crop potential of halophytes. Crit Rev Plant Sci 18:227–255
• Gregorio GB, Senadhira D, Mendoza RD (1997) Screening rice for salinity tolerance. IRRI discussion paper series no. 22. International Rice Research Institute, Manila, Philippines
• Hamada A, Shono M, Xia T, Ohta M, Hayashi Y, Tanaka A, Hayakawa T (2001) Isolation and characterization of a Na⁺/H⁺ antiporter gene from the halophyte Ariplex gmelini. Plant Mol Biol 46:43–56
• He CX, Yan JQ, Shen GX, Fu LH, Holaday AS, Auld D, Blumwald E, Zhang H (2005) Expression of an Arabidopsis vacuolar sodium/proton antiporter gene in cotton improves photosynthetic performance under salt conditions and increases fiber yield in the field. Plant Cell Physiol 46:1848–1854
• Islam SMT, Tammi RS, Singla-Pareek SL, Seraj ZI (2009) Agrobacterium-mediated transformation and constitutive expression of PgNHX1 from Pennisetum glaucum L. in Oryza sativa L. cv Binnatoa. Plant Tissue Cult Biotech 19(1):25–33
• Mahnensmith RL, Aronson PS (1985) The plasma membrane sodium–hydrogen exchanger and its role in physiological and pathophysiological processes. Circ Res 56:773–788
• Martinoia E, Maeshima M, Neuhaus E (2007) Vacuolar transporters and their essential role in plant metabolism. J Exp Bot 58:83–102
• Ohta M, Hayashi Y, Nakashima A, Hamada A, Tanaka A, Nakamura T, Hayakawa T (2002) Introduction of a Na⁺/H⁺ antiporter gene from Atriplex gmelini confers salt tolerance to rice. FEBS Lett 532:279–282
• Rajagopal D, Agarwal P, Tyagi W, Singla-Pareek SL, Reddy MK, Sopory SK (2007) Pennisetum glaucum Na⁺/H⁺ antiporter confers high level of salinity tolerance in transgenic Brassica juncea. Mol Breed 19:137–151
• Rasul NM, Ali KM, Islam R, Seraj ZI (1997) Transformation of an Indica Rice cultivar Binnatoa with Agrobacterium tumefaciens. Plant Tissue Cult 7:71–80
• Rausch T, Kirsch M, Low R, Lehr A, Viereck R, Zhigang A (1996) Salt stress responses of higher plants: the role of proton pumps and Na⁺/H⁺-antiporters. J Plant Physiol 148:425–433
• Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor
• Sottosanto JB, Gelli A, Blumwald E (2004) DNA array analyses of Arabidopsis thaliana lacking a vacuolar Na⁺/H⁺ antiporter: impact of AtNHX1 on gene expression. Plant J 40:752–771
• Verma D, Singla-Pareek SL, Rajagopal D, Reddy MK, Sopory SK (2007) Functional validation of a novel isoform of Na⁺/H⁺ antiporter from Pennisetum glaucum for enhancing salinity tolerance in rice. J Biosci 32:621–628
• Wang SY, Chen QJ, Wang WL, Wang XC, Wu MZ (2005) Production and analysis of transgenic poplar 84 K with improved salt tolerance by the introduction of OsNHX1 gene. Chin Sci Bull 50:140–144
• Wu YY, Chen QJ, Chen M, Chen J, Wang XC (2005) Salt-tolerant transgenic perennial ryegrass (Lolium perenne L.) obtained by Agrobacterium tumefaciens mediated transformation of the vacuolar Na⁺/H⁺ antiporter gene. Plant Sci 169:65–73
• Xiao BZ, Chen X, Xiang CB, Tang N, Zhang QF, Xiong LZ (2009) Evaluation of seven function-known candidate genes for their effects on improving drought resistance of transgenic rice under field conditions. Mol Plant 2:73–83
• Xue ZY, Zhi DY, Xue GP, Zhang H, Zhao YH, Xia GM (2004) Enhanced salt tolerance of transgenic wheat (Tritivum aestivum L.) expressing a vacuolar Na⁺/H⁺ antiporter gene with improved grain yields in saline soils in the field and a reduced level of leaf Na⁺. Plant Sci 167:849–859
• Yin XY, Yang AF, Zhang KW, Zhang JR (2004) Production and analysis of transgenic maize with improved salt tolerance by the introduction of AtNHX1 gene. Acta Bot Sin 7:12–20
• Yoshida S, Forno DA, Cock JH, Gomez KA (1976) Laboratory manual for physiological studies of rice, 3rd edn. IRRI, Manila
• Zhang HX, Blumwald E (2001) Transgenic salt-tolerant tomato plants accumulate salt in foliage but not in fruit. Nat Biotechnol 19:765–768
• Zhang HX, Hodson JN, Williams JP, Blumwald E (2001) Engineering salt-tolerant Brassica plants: characterization of yield and seed oil quality in transgenic plants with increased vacuolar sodium accumulation. Proc Natl Acad Sci USA 98:12832–12836