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Abstrakty
The objective of this research was to study the effect of the chelated form of the iron salt of ethylenediamine di-o-hydroxyphenylacetic acid (Fe-EDDHA) (6% Fe) on in vitro rooting of the rootstock GF-677. The iron salt of ethylenediamine tetraacetic acid (Fe-EDTA) (12% Fe) of the MS basic medium was replaced by Fe-EDDHA, which was applied in three concentrations: 93.5, 187.0 and 280 mg l⁻¹ (5.6, 11.2 and 16.8 mg l⁻¹ Fe, respectively). For each treatment of Fe-EDDHA, the effect of ascorbic acid added in four concentrations (0, 0.1, 1.0 and 10 mg l⁻¹) was studied. After 4 weeks of culture, the explants growing on the medium with 280 mg l⁻¹ Fe-EDDHA gave the best rooting results. Regarding ascorbic acid, no clear stimulating effect on rooting was found.
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Opis fizyczny
p.559-561,fig.,ref.
Twórcy
autor
- Department of Horticulture, School of Agriculture, Aristotle University, 541 24 Thessaloniki, Greece
autor
- Department of Horticulture, School of Agriculture, Aristotle University, 541 24 Thessaloniki, Greece
autor
- Department of Horticulture, School of Agriculture, Aristotle University, 541 24 Thessaloniki, Greece
autor
- Department of Agricultural Development, Democritus University of Thrace, 68200 Orestiada, Greece
autor
- Department of Horticulture, School of Agriculture, Aristotle University, 541 24 Thessaloniki, Greece
Bibliografia
- Antonopoulou C, Dimassi K, Therios I, Chatzissavvidis C (2004) The influence of radiation quality on the in vitro rooting and nutrient concentration of peach rootstock GF-677. Biologia Plantarum 48:549–553
- Antonopoulou C, Dimassi K, Therios I, Chatzissavvidis C, Tsirakoglou V (2005) Inhibitory effects of riboflavin (Vitamin B2) on the in vitro rooting and nutrient concentration of explants of peach rootstock GF-677. Sci. Hortic 106:268–272
- Chopra RN, Rashid A (1969) Induction of shoot buds in Anoectangium thomsonii Mitt by a metal chelate Fe-EDDHA. Zeitung Pflanzenphysiolog 61:199–202
- Dalton CC, Iqbal K, Turver DA (1983) Iron phosphate precipitation in Murashige and Skoog media. Physiol Plant 57:472– 476
- Dimassi-Theriou K: Factors affecting in vitro multiplication and rhizogenesis of the peach rootstock GF 677 and petunia (Petunia hybrida). PhD thesis (In Greek), Aristotle University of Thessaloniki, Greece, pp 18–20 (1989)
- Dunlap JR, Robacker KM (1988) Nutrient salts promote light-induced degradation of indole-3-acetic acid in tissue culture. Plant Physiol 88:379–382
- Foyer C, Rowell J, Walker D (1983) Measurements of the ascorbate content of spinach leaf protoplasts and chloroplasts during illumination. Planta 157:239–244
- Gaspar T, Kevers C, Hausman JF, Berthon JY, Ripetti V (1992) Practical use of peroxidase activity as a predictive marker of rooting performance of micropropagated shoots. Agronomie 12:757–765
- Halliwell B, Gutteridge JMC (1984) Oxygen toxicity, oxygen radicals, transition metals and disease. Biochem. J 219:1–14
- Hangarter RP, Stasinopoulos TC (1991) Effect of Fe-catalyzed photooxidation of EDTA on root growth in plant culture media. Plant Physiol 96:843–847
- Liso R, Innocenti AM, Bitonti MB, Arrigoni O (1988) Ascorbic acid-induced progression of quiescent center cells from G1 to S phase. New Phytol 110:469–471
- Maheshwari SC, Seth PN (1965) Induction of flowering in Wolfia microscopica by the iron salt of ethylenediamine-di-o-hydroxyphenylacetic acid (Fe-EDDHA). Zeitung Pflanzenphysiolog 55:89–91
- Molassiotis AN, Dimassi KN, Therios IN, Diamantidis GR (2003) Fe-EDDHA promotes rooting of rootstock GF-677 (Prunus amygdalus × P. persica) explants in vitro. Biol. Plant 47:141–144
- Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
- Rashid A, Street HF (1973) The development of haploid embryoids from anther cultures of Atropa belladonna L. Planta 113:263–270
- Shibli RA, Mohammad MJ, Ajlouni ZI (2002) Growth and micronutrient acquisition of in vitro grown bitter almond and sour orange in response to iron concentration from different iron chelates. J Plant Nutr 25:1599–1606
- Singh M, Krikorian AD (1980) Chelated iron in culture media. Ann Bot 46:807–809
- Thompson JE, Legge RL, Barber RF (1987) The role of free radicals in senescence and wounding. New Phytol 105:317–344
Typ dokumentu
Bibliografia
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