Integrative vectors for gene deletion and replacement

• Autorzy: Sektas M. , Gregorowicz M. , Kucharska M. , Jodelko E.
• Języki publikacji: EN

Abstrakty

EN

An improved method for gene deletion or replacement in Escherichia coli was developed. It employs a set of integrative vectors and two helper plasmids, as a temporary source of RecA and Flp activities. The integrative vectors combine several useful features including three different selection markers placed between two parallel oriented Flp recombinase target (FRT) sites. Each marker is flanked by two MCSs, for cloning the chosen homologous fragments of DNA to gene targeting. The vectors contain two properly oriented E. coli Chi sites for recombination enhancement. When required, selection markers can be excised from the chromosome resulting in unmarked strains.

Słowa kluczowe

EN

integrative vector; gene deletion; gene replacement; replacement; Escherichia coli; gene targeting

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2013
• Tom: 62
• Numer: 1
• Opis fizyczny: p.77-80,fig.,ref.

Twórcy

autor

Sektas M.

• Department of Microbiology, University of Gdansk, Wita Stwosza 59, 80-308 Gdansk, Poland

autor

Gregorowicz M.

• Department of Microbiology, University of Gdansk, Wita Stwosza 59, 80-308 Gdansk, Poland

autor

Kucharska M.

• Department of Microbiology, University of Gdansk, Wita Stwosza 59, 80-308 Gdansk, Poland

autor

Jodelko E.

• Department of Microbiology, University of Gdansk, Wita Stwosza 59, 80-308 Gdansk, Poland

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