Evaluation of ursolic acid as the main component isolated from Catharanthus roseus against hyperglycemia

• Autorzy: Thanh Tam N. , Thien D.D. , Sung T.V. , Thi Hoang Anh N. , Thuy T.T. , Trung K.H. , Xuan T.D. , Khanh T.D.
• Języki publikacji: EN

Abstrakty

EN

Ursolic acid with large amount (0.67% of dried plant weight) along with 7 compounds, namely as spatozoate (1), kaurenoic acid (2), ursonic acid (3), 3-hydroxy-11-ursen-28,13-olide (4), ursolic acid (5), vindoline (6) and mixture of β-sitosterol and stigmasterol were isolated from dichloromethane and ethyl acetate extracts which have shown anti-glucosidase activity of the whole plant of C.roseus. Some isolated compounds and their derivatives were also tested for anti-glucosidase and cytotoxicity.Ursolic acid was examined for hypoglycemic activity in alloxan-induced diabetic mice with dose of 200 and 300 mg/kg/day, respectively. The results have shown that the blood glucose level were reduced by 45.75% and 51.31% to compare with the control group. This study has confirmed that the main component of Vietnamese C. roseus has had significant anti-hyperglycemia activity.

Słowa kluczowe

EN

medicinal plant; Catharanthus roseus; medicinal property; ursolic acid; hyperglycemia; blood glucose level; antihyperglycemic activity; hypoglycemic activity; antidiabetic effect

• Wydawca: -
• Czasopismo: International Letters of Natural Sciences
• Rocznik: 2016
• Tom: 50
• Opis fizyczny: p.7-17,fig.,ref.

Twórcy

autor

Thanh Tam N.

• Institute of Chemistry, Vietnam Academy of Science and Technology, Hanoi, Vietnam

autor

Thien D.D.

• Institute of Chemistry, Vietnam Academy of Science and Technology, Hanoi, Vietnam

autor

Sung T.V.

• Institute of Chemistry, Vietnam Academy of Science and Technology, Hanoi, Vietnam

autor

Thi Hoang Anh N.

• Institute of Chemistry, Vietnam Academy of Science and Technology, Hanoi, Vietnam

autor

Thuy T.T.

• Institute of Chemistry, Vietnam Academy of Science and Technology, Hanoi, Vietnam

autor

Trung K.H.

• Department of Genetic Engineering, Agricultural Genetics Institute, Tuliem, Hanoi, Vietnam

autor

Xuan T.D.

• Department of Development Technology, Graduate School for International Development and Cooperation (IDEC), Hiroshima University, Japan

autor

Khanh T.D.

• Department of Genetic Engineering, Agricultural Genetics Institute, Tuliem, Hanoi, Vietnam

Bibliografia

• [1] N.H.Admad, R.A. Rahim, I. Mat. Catharenthus roseus aqueous extract is cytotoxic to Jurkat leukaemic T-cells but induces the proliferation of normal peripheral blood mononuclear cells. Trop Life Sci Res. 21 (2010) 101-113.
• [2] F. Afrapoli-Moradi, B. Asghari, S.Saeidnia, Y. Ajani, M. Mirjai, M. Malmir, R.D. Bazaz, A.Hadjakhoondi, P. Salehi, M. Hamburger, N. Yassa. In vitro α-glucosidase inhibitory activity of phenolic constituents from aerial parts of Polygonum hyrcanicum. Daru J Pharm Sci. 20 (2012)37.
• [3] L.Almagro, F.P. Francisco, M.A. Pedreno. Indole Alkaloids from Catharanthus roseus: Bioproduction and their effect on human health. Molecules. 20 (2015) 2973-3000.
• [4] D.T. Dam. The effects of treating hypoglycemia and diabetes of some medicinal plants. J Pharm. 6 (2012) 430.
• [5] I.D.F (International Diabetes Federation). Web: https: //www.idf.org/membership/wp/vietnam (2014; latest accession June, 2015).
• [6] P.H. Ho. Flora of Vietnam. Ho Chi Minh City: Youth Publishing House, 2000, 512 p.
• [7] W. Kang, Y. Song, X. Gu. α-glucosidase inhibitory in vitro and antidiabetic activity in vivo of Osmanthus fragrans. J Med Plant Res. 6 (2012) 2850-2856.
• [8] Y.P. Keepers, P.E. Pizao, G.J. Peter, J.V. Ark-Otte, B. Winograd, H.M. Pinedo. Comparison of the sulforhodamine B protein and tetrazolium (MTT) assays for in vitro chemosensitivity testing. Eur. J. Cancer. 27 (1991) 897-900.
• [9] R.J. Marles, N.R. Farnsworth. Antidiabetic plants and their active constitutents. Phytomedicine. 2 (1995)137-189.
• [10] A. Monks, D. Scudiero, P. Skehan, R. Shoemaker, K. Paull, D. Vistica, C. Hose, J.Langley, P.Cronise, A. Vaigro-Wolff, M. Gray-Goodrich, H. Campbell, J. Mayo, M. Boyd. Feasibility of a high-flux anticancer drug screen using a diverse panel of cultured human tumor cell lines. J Natl Cancer Inst. 83 (1991)757-766.
• [11] S.Nammi, M.K. Boini, S.D. Lodagala, R. Babu, R.B.S. Behara. The juice of fresh leaves of Catharenthus roseus Linn. Reduces blood glucose in normal and alloxan diabetic rabbits. BMC Complement Altern Med. 3 (2003) 4.
• [12] A. Natarajian, K.S.Z. Ahmed, S. Sundaresan, A. Sivaraj, K. Devi, B.S. Kumar. Effect aqueous flower extract of Cathanranthus roseus on alloxan induced diabetes in male albino rats. Inter J Pharm Sci Drug Res. 4 (2012) 150-153.
• [13] L. Li, X.D. Zhang, Y.W. Song, J.W. Liu. A microplate-based screening method for α- glucosidase inhibitors. Chin J Clin Pharmacol Ther. 10 (2005) 1128-1134.
• [14] S.C. Ohadoma, H.U. Michael. Effects of co-administration of methanol leaf extract of Catharanthus roseus on the hypoglycemic activity of metformin and glibenclamide in rats. Asian Pac J Trop Med. 4 (2014) 475-477.
• [15] M.M. Rahman, M.N. Hasan, A.K. Das, M.T. Hossain, R. Jahan, M.A. Khatun, M. Rahmatullah. Effect of Delonix regia leaf extract on glucose tolerance in glucose induced hyperglycemic mice. Afr Tradit Complement Altern Med. 8 (2011) 34-36.
• [16] M.Y. Qi, J.J. Yang, B. Zhou, D.Y. Pan, X. Sun. Study on the protective effect of ursolic acid on alloxan-induced diabetic renal injury and its underlying mechanisms. Zhonqquo Ying Yong Sheng Li Xue Za Zhi. 30 (2014) 445-448 (Chinese with abstract in English).
• [17] M.J. Siddiqui, Z. Ismail, A.F.A. Aisha, A.M.S. Abdul Majid. Cytotoxicity activity of Catharanthus roseus crude extracts and pure compounds against human coorctal carcinoma cell line. Inter J Pharm. 5 (2010) 43-47.
• [18] B. Suparb, and P. Amorn. Chemical constituents of the roots of Bridelia tomentosa Bl. J. Sci. Soc. Thailand. 17 (1991) 61-69.
• [19] N.T. Tam, D.D. Thien, T.V. Sung. Further chemical constituents of Catharanthus rosues (L.) G. Don collected from Binh Dinh province, Vietnam.Vietnamese J Chem. 52 (2014) 626-628.
• [20] D.D. Thien, N.T. Tam, D.G. Thien, N.T.H. Anh, T.V. Sung.Synthesis and cytotoxic activity of ursolic acid derivatives. Zeitschrift für Naturforschung B. 68b (2013) 201-206.
• [21] S.H. Tiong, C.Y. Looi, A. Arya, W.F. Wong, H. Hazni, M.R. Mustafa, K. Awang. Vindogentianine, a hypoglycemic alkaloid from Catharenthus roseus (L.) G.Don (Apocynaceae). Fitoterapia. 102 (2015)182-188.
• [22] R. Van der Heijden, D.I. Jacobs, W. Snoeijer, D. Hallard, R. Verpoorte. The Catharanthus alkaloids: Pharmacognosy and biotechnology. Curr Med Chem. 11 (2004)1241-1253.
• [23] P.P. Wu, K. Zhang, Y.J. Lu, P. He, S.Q. Zhao. In vitro and in vivo evaluation of the antidiabetic activity of ursolic acid derivatives. Eur J Med Chem. 80 (2014) 502-508.
• [24] R. Yanarday, H. Colac. Effect chard (Beta vulgaris L. varcicla) on blood glucose level in normal and alloxan induced diabetic rabiit. J. Ethnopharm. 4 (1998) 309-311.
• DOI References
• [2] F. Afrapoli-Moradi, B. Asghari, S. Saeidnia, Y. Ajani, M. Mirjai, M. Malmir, R.D. Bazaz, A. Hadjakhoondi, P. Salehi, M. Hamburger, N. Yassa. In vitro α-glucosidase inhibitory activity of phenolic constituents from aerial parts of Polygonum hyrcanicum. Daru J Pharm Sci. 20 (2012). 10.1186/2008-2231-20-37
• [3] L. Almagro, F.P. Francisco, M.A. Pedreno. Indole Alkaloids from Catharanthus roseus: Bioproduction and their effect on human health. Molecules. 20 (2015) 2973-3000. 10.3390/molecules20022973
• [7] W. Kang, Y. Song, X. Gu. α-glucosidase inhibitory in vitro and antidiabetic activity in vivo of Osmanthus fragrans. J Med Plant Res. 6 (2012) 2850-2856. 10.5897/jmpr11.1402
• [8] Y.P. Keepers, P.E. Pizao, G.J. Peter, J.V. Ark-Otte, B. Winograd, H.M. Pinedo. Comparison of the sulforhodamine B protein and tetrazolium (MTT) assays for in vitro chemosensitivity testing. Eur. J. Cancer. 27 (1991) 897-900. 10.1016/0277-5379(91)90142-z
• [9] R.J. Marles, N.R. Farnsworth. Antidiabetic plants and their active constitutents. Phytomedicine. 2 (1995)137-189. 10.1016/s0944-7113(11)80059-0
• [10] A. Monks, D. Scudiero, P. Skehan, R. Shoemaker, K. Paull, D. Vistica, C. Hose, J. Langley, P. Cronise, A. Vaigro-Wolff, M. Gray-Goodrich, H. Campbell, J. Mayo, M. Boyd. Feasibility of a high-flux anticancer drug screen using a diverse panel of cultured human tumor cell lines. J Natl Cancer Inst. 83 (1991). 10.1093/jnci/83.11.757
• [14] S.C. Ohadoma, H.U. Michael. Effects of co-administration of methanol leaf extract of Catharanthus roseus on the hypoglycemic activity of metformin and glibenclamide in rats. Asian Pac J Trop Med. 4 (2014) 475-477. 10.1016/s1995-7645(11)60129-6
• [15] M.M. Rahman, M.N. Hasan, A.K. Das, M.T. Hossain, R. Jahan, M.A. Khatun, M. Rahmatullah. Effect of Delonix regia leaf extract on glucose tolerance in glucose induced hyperglycemic mice. Afr Tradit Complement Altern Med. 8 (2011) 34-36. 10.4314/ajtcam.v8i1.60505
• [17] M.J. Siddiqui, Z. Ismail, A.F.A. Aisha, A.M.S. Abdul Majid. Cytotoxicity activity of Catharanthus roseus crude extracts and pure compounds against human coorctal carcinoma cell line. Inter J Pharm. 5 (2010) 43-47. 10.3923/ijp.2010.43.47
• [20] D.D. Thien, N.T. Tam, D.G. Thien, N.T.H. Anh, T.V. Sung. Synthesis and cytotoxic activity of ursolic acid derivatives. Zeitschrift für Naturforschung B. 68b (2013) 201-206. 10.5560/znb.2013-2261
• [21] S.H. Tiong, C.Y. Looi, A. Arya, W.F. Wong, H. Hazni, M.R. Mustafa, K. Awang. Vindogentianine, a hypoglycemic alkaloid from Catharenthus roseus (L. ) G. Don (Apocynaceae). Fitoterapia. 102 (2015)182-188. 10.1016/j.fitote.2015.01.019
• [23] P.P. Wu, K. Zhang, Y.J. Lu, P. He, S.Q. Zhao. In vitro and in vivo evaluation of the antidiabetic activity of ursolic acid derivatives. Eur J Med Chem. 80 (2014) 502-508. 10.1016/j.ejmech.2014.04.073

Identyfikatory

DOI

10.18052/www.scipress.com/ILNS.50.7