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2008 | 58 | 4 |
Tytuł artykułu

Effect of native enzymes on the quality of lipids in experimentally-stored rapeseed

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Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Activity and immunoreactivity of lipase, activity of lipoxygenase, acid and peroxide values were determined during the storage of rapeseed with 6, 10, 14, and 18% moisture content. The most intensive changes in lipase activity were observed during storage of rapeseed with 10% moisture content and ranged from 18.73 to 55.85 A.U./mg protein as compared to stored rapeseed with 6, 14, and 18% moisture content. During the storage there were also observed changes in lipase immunoreactivity, lipoxygenase activity, acid and peroxide values, however none of the relationships between enzymes and quality of rapeseed lipids was found to be statistically significant and therefore it can be concluded that the processes taking place during storage of rapeseed with varied moisture content show complexity and the enzymes examined in the experiment can appear to be not only factors affecting the quality of the lipid fraction.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
58
Numer
4
Opis fizyczny
p.471-476,fig.,ref.
Twórcy
autor
  • Division of Food Science, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Tuwima 10, 10-747 Olsztyn, Poland
Bibliografia
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  • 6. Grabska J., Jędrychowski L., Miaczynska A., Application of immunoblotting method to detect native lipases of rapeseed. Pol. J. Food Nutr. Sci., 1997, 6, 107–114.
  • 7. Hills M.J., Murphy D.J., Characterization of lipases from lipid bodies and microsomal membranes of erucic acid free oilseed rape (Brassica napus L.) cotyledons. Biochem J., 1988, 249, 687–693.
  • 8. Hills M.J., Beevers H., An antibody to the castor bean glyoxysomal lipase (62kDa) also binds to a 62 kD protein in extracts from many young oilseed plants. Plant Physiol., 1987, 85, 1084–1088.
  • 9. James J.J., Lakshmi B.S., Raviprasad V., Ananth M.J., Kangueane P., Gautam P., Insights from molecular dynamics simulations into pH-dependent enantioselective hydrolysis of ibuprofen esters by Candida rugosa lipase. Protein Engin., 2003, 16, 1017–1024.
  • 10. Khalyafa A., Kermasha S., Alli I., Partial purification and characterization of lipoxygenase of canola seed (Brassica napus var. Westar). J. Agric. Food Chem., 1990, 38, 2003–2008.
  • 11. Lawrence R.C., Fryer T.F., Reiter B., Rapid method for the quantitative estimation of microbial lipases. Nature, 1967, 25, 1264–1265.
  • 12. Lin Y.H., Huang A.H.C., Lipase in lipid bodies of cotyledons of rape and rape and mustard seedlings. Arch. Biochem. Biophys., 1983, 225, 360–369.
  • 13. Lima V.M.G., Krieger N., Mitchell D.A., Fontana J.D., Activity and stability of a crude lipase from Penicillium aurantiogriseum in aqueous media and organic solvents. Biochem. Engin. J., 2004, 18, 65–71.
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  • 16. Polish Standard PN 74/R-66165, Grain of oil plants. Determination of the acid value of fat (in Polish).
  • 17. Polish Standard PN 84/A-86918. Edible vegetable fats. Testing methods. Determination of peroxides content (in Polish).
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  • 19. Rodriguez-Rosales M.P., Kerkeb L., Ferrol N., Donaire J.P., Lipoxygenase activity and lipid composition of cotyledons and oil bodies of two sunflower hybrids. Plant Physiol. Biochem., 1998, 36, 285–291.
  • 20. Secundo F., Carrea G., Tarabiono C., Brocca S., Lotti M., Activity and enantioselectivity of wildtype and lid mutated Candida rugosa lipase isoform 1 in organic solvents. Biotechnol. Bioengin., 2004, 86, 236–240.
  • 21. Shiiba K., Negishi Y., Okada K., Nagao S., Purification and characterization of lipoxygenase isozymes from wheat germ. Cereal Chem., 1991, 68, 115–122.
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Bibliografia
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