Structure of leaves and phenolic acids in Kalanchoe daigremontiana Raym.-Hamet and H. Perrier

• Autorzy: Chernetskyy W. , Wozniak A. , Skalska-Kaminska A. , Zuraw B. , Blicharska E. , Rejdak R. , Donica H. , Weryszko-Chmielewska E.
• Języki publikacji: EN

Abstrakty

EN

Kalanchoë daigremontiana leaves contain phenolic compounds, which are one of the determinants of plant therapeutic properties. Light and scanning electron microscopes were used to analyse the structure of leaves. The main aims of the study included the analysis of the anatomy of leaves, localisation of phenolic compounds, and identification of phenolic acids. The thickness of the amphistomatic leaf blades, the number, the size of stomata, and the value of stomatal index, as well as the structure of the parenchyma cells have indicated that K. daigremontiana is adapted to arid environments. The histochemical assays revealed the presence of phenolic idioblasts in the leaf blades and petioles. The idioblasts were located in the epidermis, subepidermal layer, a deeper portion of the mesophyll, and in the sheaths of vascular bundles. The phytochemical analyses of leaves demonstrated the presence of gallic, ferulic, caffeic, p-coumaric, and protocatechuic acids in the form of esters. We carried out the research of the anatomical structure of K. daigremontiana leaves, which has been insufficiently documented to date. We have also revealed new localisation of phenolic compounds in the leaf tissues of this species.

Słowa kluczowe

EN

ornamental plant; Kalanchoe daigremontiana; leaf structure; plant tissue; phenolic compound; phenolic acid; idioblast; therapeutic activity; plant research

• Wydawca: -
• Czasopismo: Acta Scientiarum Polonorum. Hortorum Cultus
• Rocznik: 2018
• Tom: 17
• Numer: 4
• Opis fizyczny: p.137-155,fig.,ref.

Twórcy

autor

Chernetskyy W.

• Botanical Garden, Maria Curie-Sklodowska University, Sławinkowska 3, 20-810 Lublin, Poland

autor

Wozniak A.

• Department of General Ophthalmology, Medical University of Lublin, Chmielna 1, 20-079 Lublin, Poland

autor

Skalska-Kaminska A.

• Department of Analytical Chemistry, Medical University of Lublin, Chodzki 4a, 20-093 Lublin, Poland

autor

Zuraw B.

• Department of Botany, University of Life Sciences in Lublin, Akademicka 15, 20-950 Lublin, Poland

autor

Blicharska E.

• Department of Analytical Chemistry, Medical University of Lublin, Chodzki 4a, 20-093 Lublin, Poland

autor

Rejdak R.

• Department of General Ophthalmology, Medical University of Lublin, Chmielna 1, 20-079 Lublin, Poland

autor

Donica H.

• Department of Clinical Biochemistry, Medical University of Lublin, Staszica 16, 20-081 Lublin, Poland

autor

Weryszko-Chmielewska E.

• Department of Botany, University of Life Sciences in Lublin, Akademicka 15, 20-950 Lublin, Poland

Bibliografia

• Abdel-Raouf, H.S. (2012). Anatomical traits of some species of Kalanchoë (Crassulaceae) and their taxonomic value. Ann. Agric. Scien., 57(1), 73–79. DOI: 10.1016/j.aoas.2012.03.002.
• Bačić, T., Ljubešc, N., Užarević, Z., Grgić, L., Roša, J. (2004). TEM investigation of tannins and chloroplast structure in needles of damaged silver fir trees (Abies alba Mill.). Acta Biol. Cracov. Ser. Bot., 46, 145–149.
• Balsamo, R., Uribe, E. (1988). Leaf anatomy and ultrastructure of the Crassulacean-acid-metabolism plant Kalanchoë daigremontiana. Planta, 173(2), 183–189. DOI: 10.1007/BF00403009.
• Batygina, T.B., Bragina, E.A., Titova, G.E. (1996). Morphogenesis of propagules in viviparous species Bryophyllum daigremontianum and B. calycinum. Acta Soc. Bot. Pol. 65(1-2), 127–133. DOI: 10.5586/asbp.1996.022.
• Bogucka-Kocka, A., Zidon, Ch., Kasprzycka, M., Szymczak, G., Szewczyk, K. (2016). Phenolic acid content, antioxidant and cytotoxic activieties of four Kalanchoë species. Saudi J. Biol. Sci. DOI: 10.1016/j.sjbs.2016.01.037 [in press].
• Boiteau, P., Allorge-Boiteau, L. (1995). Kalanchoë de Madagascar. Systématique, écophysiologie et phytochemie. Karthala, Paris.
• Brzezicka, E., Karwowska, K., Kozieradzka-Kiszkurno, M., Chernetskyy, M. (2015). Leaf micromorphology of Kalanchoë laciniata (Crassulaceae). Mod. Phytomorphol., 8, 49–52. DOI: 10.5281/zenodo.159829.
• Chauser-Volfson, E., Shen, Z., Hu, Z., Gutterman, Y. (2002). Anatomical structure and distribution of secondary metabolites as a peripheral defence strategy in Aloë hereroensis leaves. Bot. J. Linn. Soc., 138, 107–116. DOI: 10.1046/j.1095-8339.2002.00012.x.
• Chernetskyy (Czernecki), M. (2006). Mikromorfologia epidermy liści wybranych gatunków Kalanchoë Adans. Rocznik EKPiUU (Lublin), 3, 371–380 [in Polish].
• Chernetskyy, M. (2012). The role of morpho-anatomical traits of the leaves in the taxonomy of Kalanchoideae Berg. subfamily (Crassulaceae DC.). Modern Phyt., 1, 15–18. DOI: 10.5281/zenodo.162711.
• Chernetskyy, M., Weryszko-Chmielewska, E. (2008). Structure of Kalanchoë pumila Bak. leaves (Crassulaceae DC.). Acta Agrobot., 61(2), 11–24. DOI: 10.5586/aa.2008.029.
• Chovanskaâ, N.V. (1970). Charakter fotoperiodičeskoj lokalizacii v vegetativnom razmnoženii Kalanchoë daigremontiana. In: Sbornik trudov po agronomičeskoj fizike. Vol. 21. Svetofiziologiâ rastenij, Moškov, B.S. (ed.). Kolos, Leningrad, 67–70.
• Costa, S.S. Jossang, A. Bodo, B. (1995). Propriétés biologiques et phytochimie des Kalanchoë. In: Kalanchoë de Madagaskar. Systématique, écophysiologie et phytochemie, Boiteau, P., Allorge-Boiteau, L. (eds). Karthala, Paris, 219–235.
• Descoings, B., (2003). Kalanchoë. In: Illustrated Handbook of Succulent Plants – Crassulaceae, Eggli, U. (ed.), Springer-Verlag, Berlin–Heidelberg–New York, 143–181.
• Descoings, B. (2006). Le genre Kalanchoë (Crassulaceae): structure et définition. J. Bot. Bull. Soc. Bot. Fr., 33, 3–28.
• Gabe, M. (1968). Techniques Histologiques. Masson et Cie, París.
• Joseph, B., Sridhar, S., Sankarganesh, Justinraj, Edwin, B.T. (2011). Rare medicinal plant – Kalanchoë pinnata. Res. J. Microbiol., 6(4), 322–327. DOI: 10.3923/jm.2011.322.327.
• Kamboj, A., Saluja, A.K. (2009). Bryophyllum pinnatum (Lam.) Kurz.: Phytochemical and pharmacological profile: A reviev. Pharmacogn. Rev., 3, 364–374.
• Kaul, R.B. (1977). The role of the multiple epidermis in foliar succulence of Peperomia (Piperaceae). Bot. Gaz., 138(2), 213–218. DOI: 10.1086/336917.
• Kołodziejczyk-Czepas, J., Sieradzka, M., MoniuszkoSzajwaj, B., Pecio, Ł., Ponczek, M.B., Nowak, P., Stochmal, A. (2017). Bufadienolides from Kalanchoë daigremontiana as thrombin inhibitors – In vitro and in silico study. Int. J. Biol. Macromol., 99, 141–150. DOI: 10.1016/j.ijbiomac.2017.02.051.
• Kopcewicz, J., Lewak, S. (ed.). (2005). Fizjologia roślin. Wyd. Nauk. PWN, Warszawa.
• Kruk, J., Pisarski, A., Szymańska, R. (2011). Novel vitamin E forms in leaves of Kalanchoë daigremontiana and Phaseolus coccineus. J. Plant. Physiol., 168, 2021–2027. DOI: 10.1016/j.jplph.2011.06.015.
• Leal-Costa, M.W., Nascimento, L.B.S., Moreira, N.S., Reinert, F., Costa, S.S., Lage, C.L.S., Tavares, W.S. (2010). Influence of blue light on the leaf morphoanatomy of in vitro Kalanchoë pinnata (Lamarck) Persoon (Crassulaceae). Microsc. Microanal., 16, 576–582. DOI: 10.1017/S1431927610000279.
• Legramandi, V.H.P. (2011). Kalanchoë gastonis-bonnieri Raym.-Hamet et H. Perrier e Kalanchoë pinnata Pers. (Crassulaceae): atividade antifúngica e estudo farmacognóstico comparativo. Dissertação apresentada ao Programa de Pós-Graduação em Ciências Farmacêuticas, da Faculdade de Ciências Farmacêuticas, UNESP, como parte dos requisitos para obtenção do Título de Mestre em Ciências Farmacêuticas, Araraquara – SP. Available: ttps://repositorio.unesp.br/bitstream/handle/11449/96246/legramandi_vhp_me_arafcf.pdf?sequence=1 [date of access: 16.01.2018].
• Lyubimov, V.Yu., Atakhanov, B.O., Bil, K.Ya. (1986). Adaptation of photosynthetic apparatus in plants of the Kara-Kum desert to extreme conditions. Russ. J. Plant Physiol., 33(5), 888–895.
• Maarseveen, C., Jetter, R. (2009). Composition of the epicuticular and intracuticular wax layers on Kalanchoë daigremontiana (Hamlet et Perr. De la Bathie) leaves. Phytochemistry, 70(7), 899–906. DOI: 10.1016/j.phytochem.2009.04.011.
• Metcalfe, C.R., Chalk, L. (1957). Anatomy of the dicotyledons. Vol. 2. Clarendon Press, Oxford.
• Moreira N.S., Nascimento, L.B.S., Leal-Costa M.V., Tavares E.S. (2012). Comparative anatomy of leaves of Kalanchoë pinnata and K. crenata in sun and shade conditions, as a support for their edentification. Rev. Bras. Farmacogn. Braz. J. Pharmacogn., 22(5), 929-936. DOI: 10.1590/S0102-659X2012005000056.
• Muzitano, M.F., Tinoco, L.W., Guette, C., Kaiser, C.R., Rossi-Bergmann, B., Costa, S.S. (2006). The antileishmanial activity assessment of unusual flavonoids from Kalanchoë pinnata. Phytochemistry, 67, 2071–2077. DOI: 10.1016/j.phytochem.2006.06.027.
• Nowak, S., Rychlińska, I. (2012). Phenolic acids in the flowers and leaves of Grindelia robusta Nutt. and Grindelia squarrosa Dun. (Asteraceae). Acta Pol. Pharm., 69, 693–698.
• Nuzhyna, N.V., Gaydarzhy, M.M. (2015). Comparative characteristics of anatomical and morphological adaptations of plants of two subgenera Haworthia Duval to arid environmental conditions. Acta Agrobot., 68(1), 23–31. DOI: 10.5586/aa.2015.006.
• Oleszek, W., Głowniak, K., Leszczyński, B. (eds) (2001). Biochemiczne oddziaływania środowiskowe. Akad. Med., Lublin.
• Pattewar, S.V. (2012). Kalanchoë pinnata: phytochemical and pharmacological profile, IJPSR 3(4), 993–1000. DOI: 10.7439/ijpp.v2i1.223.
• Ramalingam, K., Ravindranath, M.H. (1970). Histochemical significance of green metachromasia to toluidine blue. Histochemie, 24(4), 322–327. DOI: 10.1007/BF00278217.
• Sarwa, A. (2001). Wielki leksykon roślin leczniczych. Książka i Wiedza, Warszawa.
• Sazhina, N.N., Lapshin, P.V., Zagoskina, N.V., Korotkova, E.I., Misin, V.M. (2014). A comparative analysis of the antioxidant activity of Kalanchoë juices. Russ. J. Bioorg. Chem., 40(7), 771–776. DOI: 10.1134/S1068162014070152.
• Scholtysik, G., Wagner, H., Fische, M., Rüegg, U. (1986). Cardiac glycoside-like effects of a bufadienolide extracted from Kalanchoë daigremontiana. In: Cardiac Glycosides, Erdmann, E., Greef, K., Skou, J.C. (eds). Steinkopff, Heidelberg, 1785–1985.
• Sharma, G.K, Dunn, D.B. (1968). Effect of environment on the cuticular features in Kalanchoë fedtschenkoi. Bull. Torrey Bot. Club, 95(5), 464–473. DOI: 10.2307/2483478.
• Sharma, G.K., Lewis, P.B. (1987). Cuticular differentiation in Kalanchoë fedtschenkoi and Kalanchoë daigremontianum (Crassulaceae). J. Tenn. Acad. Sci., 62(4), 97–98.
• Sowa, I., Kocjan, R., Wójciak-Kosior, M., Świeboda, R., Zajdel, D., Hajnos, M. (2013). Physicochemical properities of silica gelcoated with a thin layer of polyaniline (PANI) and its application in non-suppressed ion chromatography. Talanta, 115, 451–456. DOI: 10.1016/j.talanta.2013.05.071.
• Strobel, D.W., Sunberg, M.D. (1983–1984). Stomatal density in leaves of various xerophytes-preliminary study. J. Minn. Acad. Sci., 49(2), 7–9.
• The Polish Pharmacopoeia IX (2011). Urząd Rejestracji Produktów Leczniczych, Wyrobów Medycznych i Produktów Biobójczych (URPLWMiPB), Warsaw.
• Troll, W. (1959). Allgemeine Botanik. Ferdinand Enke Verlag, Stuttgart.
• Tyszczuk, K., Skalska-Kamińska, A., Woźniak, A. (2011). Voltammetric method using a lead film electrode for the determination of caffeic acid in a plant material. Food Chem., 125, 1498–1503. DOI: 10.1016/j.foodchem.2010.10.075.
• Weryszko-Chmielewska, E., Chernetskyy, M. (2005). Structure of trichomes from the surface of leaves of some species of Kalanchoë Adans. Acta Biol. Cracov. Ser. Bot., 47(2), 15–22.
• Wójciak-Kosior, M., Skalska, A. (2006). Thin-layer chromatography of phenolic acids on aminopropylsilica. J. Planar Chromatogr., 19, 200–203. DOI: 10.1556/JPC.19.2006.3.5.

Identyfikatory

DOI

10.24326/asphc.2018.4.13