Separation of pure bovine IgG1, IgG2, IgM and IgA and production of antibodies to them

• Autorzy: Grundboeck-Jusko J. , Bicka L. , Bozik K. , Kozaczynska B. , Kuzmak J.
• Języki publikacji: EN

Abstrakty

EN

Pure IgG₁ and IgG₂ globulins were obtained by means of precipitation with ammonium sulphate and subsequent fractionation in DEAE Sephadex A-50 column. The IgM globulin was isolated by means of chromatography on Sephadex G-200 and DEAE-cellulose. Antibodies to these proteins were produced by immunization of rabbits and goats. Purity of proteins and monovalence of antibodies were checked up by means of immunoelectrophoresis and polyacrylamide gel electrophoresis.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 1987-1988
• Tom: 30-31
• Numer: 1-4
• Opis fizyczny: p.39-46,fig.,ref.

Twórcy

autor

Grundboeck-Jusko J.

• Department of Biochemistry, The Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Bicka L.

• Department of Biochemistry, The Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Bozik K.

• Department of Biochemistry, The Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Kozaczynska B.

• Department of Biochemistry, The Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Kuzmak J.

• Department of Biochemistry, The Veterinary Research Institute, 24-100 Pulawy, Poland

Bibliografia

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