Application of real-time reverse transcription polymerase chain reaction for the detection of SVDV

• Autorzy: Niedbalski W.
• Języki publikacji: EN

Abstrakty

EN

Application of real-time RT-PCR (rRT-PCR) for detection of swine vesicular disease virus (SVDV) in samples of archival SVDV isolates and clinical samples collected from SVDV infected pigs was described. A primer set that targets the IRES region of the SVDV genome and TaqMan probe specific for a highly conserved region in SVDV RNA IRES region were used. The assay detected viral RNA in all tested archival strains of SVDV isolated in Europe during years 1972-73 and 1992 as well as in clinical samples collected from experimentally infected pigs. The rRT-PCR can provide quantitative and qualitative information and is more sensitive and faster to perform than the conventional RT-PCR.

Słowa kluczowe

EN

application; real-time reverse transcription polymerase chain reaction; detection; swine vesicular disease; pig; animal disease; Enterovirus; Picornaviridae; identification

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2009
• Tom: 12
• Numer: 1
• Opis fizyczny: p.119-121,fig.,ref.

Twórcy

autor

Niedbalski W.

• Department of Foot-and-Mouth Disease, National Veterinary Research Institute, 98-220 Zdunska Wola, Poland