Ekspresja białka Ki-67, PCNA i indeksu apoptotycznego w przebiegu nieswoistego zapalenia jelit u psów z oceną ich ich wartości diagnostycznej

• Autorzy: Rychlik A. , Nowicki M. , Szweda M. , Kaczmar E.

Warianty tytułu

EN

Expression of Ki-67, PCNA markers and the apoptotic index in the inflammatory bowel disease in dogs and its diagnostic values

• Języki publikacji: PL

Abstrakty

EN

The aim of this study was to determine the expression of Ki-67 and PCNA proliferative antigens and the apoptotic index in dogs with various degrees of severity of inflammatory bowel disease (IBD), and to evaluate the applicability of these markers in diagnosing the disease. The study was performed on 28 dogs of different breeds, both sexes, with body weights of 10 to 20 kg, aged 2 to 10 years. The animals diagnosed with IBD were divided into four groups of 7 dogs each, including three groups characterized by various degrees of severity of IBD and a control group. The expression of Ki-67 and PCNA antigens was determined immunohistochemically, and the apoptotic index was expressed as the number of TUNEL-positive lamina propria cells in the duodenum, jejunum and the colon. Regardless of its form, IBD affected the expression of the analyzed proliferation markers and apoptosis in duodenal, jejunal and colonic mucosa, but most of the analyzed parameters were not correlated with the severity of the disease. The severity of IBD was correlated only with the expression of Ki-67 in duodenal villi. The results of the study point to the limited applicability PCNA and the apoptotic index in differentiating and evaluating the progression of IBD in dogs. The estimation of Ki-67 expression may be useful in the evaluation of the severity of IBD in dogs.

Słowa kluczowe

PL

psy; choroby przewodu pokarmowego; nieswoiste zapalenie jelit; przebieg choroby; bialko Ki-67; antygen proliferacji jadra; ekspresja bialek; ekspresja antygenow; indeks apoptotyczny; oznaczanie; wartosc diagnostyczna

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2017
• Tom: 73
• Numer: 09
• Opis fizyczny: s.567-571,rys.,tab.,bibliogr.

Twórcy

autor

Rychlik A.

• Katedra Diagnostyki Klinicznej, Wydział Medycyny Weterynaryjnej, Uniwersytet Warmińsko-Mazurski w Olsztynie, ul.Oczapowskiego 14, 10-719 Olsztyn

autor

Nowicki M.

• Katedra Diagnostyki Klinicznej, Wydział Medycyny Weterynaryjnej, Uniwersytet Warmińsko-Mazurski w Olsztynie, ul.Oczapowskiego 14, 10-719 Olsztyn

autor

Szweda M.

• Katedra Diagnostyki Klinicznej, Wydział Medycyny Weterynaryjnej, Uniwersytet Warmińsko-Mazurski w Olsztynie, ul.Oczapowskiego 14, 10-719 Olsztyn

autor

Kaczmar E.

• Katedra Diagnostyki Klinicznej, Wydział Medycyny Weterynaryjnej, Uniwersytet Warmińsko-Mazurski w Olsztynie, ul.Oczapowskiego 14, 10-719 Olsztyn

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Identyfikatory

DOI

10.21521/mw.5769