Occurrence of genes encoding virulence factors in Bordetella bronchiseptica strains isolated from infected and healthy pigs

• Autorzy: Stepniewska K. , Markowska-Daniel I.
• Języki publikacji: EN

Abstrakty

EN

The objective of the study was to determine genotypie profiles of Bordetella bronchiseptica (Bbr) strains, based on the occurrence of genes encoding virulence factors, such as flagella (flα), dermonecrotoxin (dnt), and exogenous ferric siderophore receptor (bfrZ), using PCR. 209 tested Bbr strains were obtained from Polish swine herds with different health status (with progressive atrophic rhinitis - PAR, suspected for PAR, and unknown). In total, seven different Bbr genotypes were determined. In 39.2% of Bbr isolates all three genes were present. In 41.1% of the isolates only two genes were detected. The most common genotype dnt+bfrZ-flα+ was present in 60 (28.5%) Bbr strains, 65% of them were obtained from farms with PAR. Twenty five (12%) Bbr isolates were identified as dnt-bfrZ+flα+ genotype and, as above, they were more frequently isolated from clinical cases of disease (84%). Among 31 (14.8%) strains only flα gene was evident, and in nine (4.3%) only dní gene was present. There were no Bbr strains with bfrZ gene only. These results confirm the heterogenicity among Bbr strains.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2012
• Tom: 56
• Numer: 4
• Opis fizyczny: p.483-487,ref.

Twórcy

autor

Stepniewska K.

• Department of Swine Disease, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Markowska-Daniel I.

• Department of Swine Disease, National Veterinary Research Institute, 24-100 Pulawy, Poland

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