Effect of culture conditions, cytokinins, methyl jasmonate and salicylic acid on the biomass accumulation and production of withanolides in multiple shoot culture of Withania somnifera (L.) Dunal using liquid culture

• Autorzy: Sivanandhan G , Rajesh M. , Arun M. , Jeyaraj M. , Dev G. K. , Arjunan A. , Manickavasagam M. , Muthuselvam M. , Selvaraj N. , Ganapathi A.
• Języki publikacji: EN

Abstrakty

EN

The influence of cytokinins and culture conditions including medium volume, harvest time and elicitation with abiotic elicitors (SA/MeJ) have been studied for the optimal production of biomass and withanolides in the multiple shoot culture of Withania somnifera. Elicitation of shoot inoculum mass (2 g 1-1 FW) with SA at 100 μM in the presence of 0.6 mg 1-1 BA and 20 mg 1-1 spermidine for 4 h exposure time at the 4th week in 20 m1 liquid medium recorded higher withanolides production (withanolides A [8.48 mg g-1 DW], withanolides B [15.47 mg g-1 DW], withaferin A [29.55 mg g-1 DW] and withanone [23.44 mg g-1 DW]), which were 1.14 to 1.18- fold higher than elicitation with MeJ at 100 μM after 5 weeks of culture. SA-elicited cultures did not exhibit much variation in biomass accumulation when compared to control. This cytokinin induces and SA-elicited multiple shoot culture protocol provides a potential alternative for the optimal production of biomass and withanolides utilizing liquid culture.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2013
• Tom: 35
• Numer: 03
• Opis fizyczny: p.715-728,fig.,ref

Twórcy

autor

Sivanandhan G

• Department of Biotechnology and Genetic Engineering, School of Biotechnology, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India

autor

Rajesh M.

• Department of Biotechnology and Genetic Engineering, School of Biotechnology, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India

autor

Arun M.

• Department of Biotechnology and Genetic Engineering, School of Biotechnology, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India

autor

Jeyaraj M.

• Department of Biotechnology and Genetic Engineering, School of Biotechnology, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India

autor

Dev G. K.

• Department of Biotechnology and Genetic Engineering, School of Biotechnology, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India

autor

Arjunan A.

• Department of Biotechnology and Genetic Engineering, School of Biotechnology, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India

autor

Manickavasagam M.

• Department of Biotechnology and Genetic Engineering, School of Biotechnology, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India

autor

Muthuselvam M.

• Department of Biotechnology and Genetic Engineering, School of Biotechnology, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India

autor

Selvaraj N.

• Periyar E. V. R College (Autonomous), Tiruchirappalli 620 023, Tamil Nadu, India

autor

Ganapathi A.

• Department of Biotechnology and Genetic Engineering, School of Biotechnology, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India

Bibliografia

• Adelberg J (2004) Plant growth and sugar utilization in an agitated, thin-film liquid system for micropropagation. In Vitro Cell Dev Biol Plant 40:245–250
• Bais HP, Ravishankar GA (2003) Synergistic effect of auxins and polyamines in hairy root of Chicorium intybus L. during growth, coumarin production, and morphogenesis. Acta Physiol Plant 25:193–208
• Carmen SJM, Ballester A, Vieitez AM (2001) Effect of thidiazuron on multiple shoot induction and plant regeneration from cotyledonary nodes of chestnut. J Hortic Sci Biotechnol 76:588–595
• Chatterjee S, Srivastava S, Khalid A, Singh N, Sangwan RS, Sidhu OP, Roy R, Khetrapal CL, Tuli R (2010) Comprehensive metabolic fingerprinting of Withania somnifera leaf and root extracts. Phytochemistry 71:1085–1094
• Coste A, Vlase L, Halmagyi A, Deliu C, Coldea G (2011) Effects of plant growth regulators and elicitors on production of secondary metabolites in shoot cultures of Hypericum hirsutum and Hypericum maculatum. Plant Cell Tiss Org Cult 106:279–288
• Couee I, Hummel I, Sulmon C, Gouesbet G, Amrani AE (2004) Involvement of polyamines in root development. Plant Cell Tiss Org Cult 76:1–10
• Diwan R, Malpathak N (2010) Bioprocess optimization of furanocoumarin elicitation by medium renewal and re-elicitation: a perfusion-based approach. Appl Biochem Biotech 163:756–764
• Furmanowa M, Gajdzis-Kuls D, Ruszkowska J, Czarnocki Z, Obidoska G, Sadowska A, Rani R, Upadhyay SN (2001) In vitro propagation of Withania somnifera and isolation of withanolides with immunosuppressive activity. Planta Med 67:146–149
• Heble MR (1985) Multiple shoot cultures: a viable alternative in vitro system for the production of known and new biologically active plant constituents. In: Neumann KH, Barz W, Reinhard E (eds) Primary and secondary metabolism of plant cell cultures. Springer, Berlin, pp 281–289
• Ivanova M, Van Staden J (2010) Influence of gelling agent and cytokinins on the control of hyperhydricity in Aloe polyphylla. Plant Cell Tiss Organ Cult 23:5–7
• Jo UA, Murthy HN, Hahn EJ, Paek KY (2008) Micropropagation of Alocasia amazonica using semisolid and liquid cultures. In Vitro Cell Dev Biol Plant 44:26–32
• Kaileh M, Vanden Berghe W, Heyerick A, Horion J, Piette J, Libert C, De Keukeleire D, Essawi T, Haegeman G (2007) Withaferin a strongly elicits IkappaB kinase beta hyperphosphorylation concomitant with potent inhibition of its kinase activity. J Biol Chem 282:4253–4264
• Kang S, Jung H, Kang Y, Yun D, Bahk J, Yang J, Choi M (2004) Effects of methyl jasmonate and salicylic acid on the production of tropane alkaloids and the expression of PMT and H6H in adventitious root cultures of Scopolia parviflora. Plant Sci 166:745–751
• Kim OT, Kim MY, Hong MH, Ahn JC, Hwang B (2004) Stimulation of asiaticoside accumulation in the whole plant cultures of Centella asiatica (L.) Urban by elicitors. Plant Cell Rep 23:339–344
• Kulkarni AA, Thengane SR, Krishnamurthy KV (1996) Direct in vitro regeneration of leaf explants of Withania somnifera (L.) Dunal. Plant Sci 119:163–168
• Kulkarni AA, Thengane SR, Krishnamurthy KV (2000) Direct shoot regeneration from node, internode, hypocotyl and embryo explants of Withania somnifera. Plant Cell Tiss Org Cult 62:203–209
• Liu CZ, Guo C, Wang Y, Ouyang F (2003) Factors influencing artemisinin production from shoot cultures of Artemisia annua L. World J Microbiol Biotechnol 19:535–538
• Martin-Tanguy J (2001) Metabolism and function of polyamines in plants: recent development (new approaches). Plan Growth Regul 34:135–148
• Mehrotra S, Goel MK, Kukreja AK, Mishra BN (2007) Efficiency of liquid culture systems over conventional micropropagation: a progress towards commercialization. Afr J Biotechnol 6:1481–1492
• Mirjalili MH, Fakhr-Tabatabaei SM, Bonfill M, Alizadeh H, Cusido RM, Ghassempour A, Palazon J (2009) Morphology and withanolide production of Withania coagulans hairy root cultures. Eng Life Sci 9:197–204
• Mishra LC, Singh BB, Dagenais S (2000) Scientific basis for the therapeutic use of Withania somnifera (ashwagandha): a review. Altern Med Rev 5:334–346
• Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497
• Nitsch JP, Nitsch C (1969) Haploid plants from pollen grains. Science 163:85–87
• Park SY, Murthy HN, Paek KY (2000) Mass multiplication of protocorm-like bodies (PLBs) using bioreactor system and subsequent plant regeneration in Phalaenopsis. Plant Cell Tiss Org Cult 63:67–72
• Pati PK, Kaur J, Singh P (2010) A liquid culture system for shoot proliferation and analysis of pharmaceutically active constituents of Catharanthus roseus (L.) G. Don. Plant Cell Tiss Org Cult 105:299–307
• Rao MV, Lee H, Creelman RA, Mullet JE, Davis KR (2000) Jasmonic acid signaling modulates ozone-induced hypersensitive cell death. Plant Cell 12:1633–1646
• Ray S, Jha S (2001) Production of withaferin A in shoot cultures of Withania somnifera. Planta Med 67:432–436
• Roja G, Heble MR, Sipahimalani AT (1991) Tissue cultures of Withania somnifera: morphogenesis and withanolide synthesis. Phyto Res 5:185–187
• Sandal I, Bhattacharya A, Ahuja PS (2001) An efficient liquid culture system for tea proliferation. Plant Cell Tiss Org Cult 65:75–80
• Sangwan RS, Chaurasia ND, Misra LN, Lal P, Uniyal GC, Sharma R, Sangwan NS, Suri KA, Qazi GN, Tuli R (2007a) Phytochemical variability in commercial herbal products and preparations of Withania somnifera (Ashwagandha). Curr Sci 86:461–465
• Sangwan RS, Chaurasiya ND, Lal P, Misra L, Uniyal GC, Tuli R, Sangwan NS (2007b) Withanolide A biogeneration in in vitro shoot cultures of Ashwagandha (Withania somnifera Dunal), a main medicinal plant in Ayurveda. Chem Pharm Bull 55:1371–1375
• Selvaraj N, Vasudevan A, Manickavasagam M, Kasthurirengan S, Ganapathi A (2007) High frequency shoot regeneration from cotyledon explants of cucumber via organogenesis. Sci Hortic 112:2–8
• Sen J, Sharma AK (1991) Micropropagation of Withania somnifera from germinating seeds and shoot tips. Plant Cell Tiss Org Cult 26:71–73
• Sharada M, Ahuja A, Suri KA, Vij SP, Khajuria RK, Verma V, Kumar A (2007) Withanolide production by in vitro cultures of Withania somnifera and its association with differentiation. Biol Plant 51:161–164
• Sharada M, Ahuja A, Vij SP (2008) Applications of biotechnology in Indian ginseng (ashwagandha): progress and prospects. In: Kumar A, Sopory SK (eds) Recent advances in plant biotechnology and its applications. I. K. International Pvt Ltd, New Delhi, pp 645–667
• Sirvent TM, Gibson DM (2002) Induction of hypericins and hyperforin in Hypericum perforatum L. in response to biotic and chemical elicitors. Physiol Mol Plant Path 60:311–320
• Sivanandhan G, Mariashibu TS, Arun M, Rajesh M, Kasthurirengan S, Selvaraj N, Ganapathi A (2011) The effect of polyamines on the efficiency of multiplication and rooting of Withania somnifera (L.) Dunal and content of some withanolides in obtained plants. Acta Physiol Plant 33:2279–2288
• Sivanandhan G, Arun M, Mayavan S, Rajesh M, Mariashibu TS, Manickavasagam M, Selvaraj N, Ganapathi A (2012) Chitosan enhances withanolides production in adventitious root cultures of Withania somnifera (L.) Dunal. Ind Crop Prod 37:124–129
• Yu PLC, El Olemy MM, Stohs ST (1974) A phytochemical investigation of Withania somnifera tissue cultures. J Nat Prod 37:593–597
• Yu KW, Gao W, Hahn EJ, Paek KY (2002) Jasmonic acid improving ginsenoside accumulation in adventitious root culture of Panax ginseng C.A. Meyer. Biochem Eng J 11:211–215

Uwagi

rekord w opracowaniu