Wykorzystanie nasienia sortowanego u zwierząt gospodarskich - skala, efektywność inseminacji, czynniki ryzyka

• Autorzy: Jaskowski B.M. , Wozna M. , Gehrke M.

Warianty tytułu

EN

Sex-sorted sperm in farm animals: scale of use, the effectiveness of insemination, risk factors

• Języki publikacji: PL

Abstrakty

EN

The aim of the study was to present the scale of use, risk factors and possibilities, which sorter semen gives in biotechnics used in reproduction of cattle. Modern sorters allow for the evaluation of 6 million X and Y spermatozoa per hour. Sex-sorted semen, which is commercially used, contains 2.1 × 106 of spermatozoa. It is used mostly in AI of milk heifers, mainly in large cattle herds. Sorted semen containing Y spermatozoa is sold less often in the world than the one with X spermatozoa. The percentage of the desired sex of the young is higher than 90. The pregnancy rate after application of sorted semen is about 20–25% lower than after insemination of non-sorted semen and depends on a number of factors. The main factors are: breed of female, service number, the herd of origin, the depth of semen deposition, the bull producing semen, ambient temperature and technical parameters during sperm sorting. A number of methods have been developed to improve conception rate, including timed artificial insemination (TAI) and synchronization of heat and ovulation. Results of donor inseminations with the use of sorter semen are presented, with the lower percentage of embryos suitable for the transfer and embryos of the highest quality highlighted. Previous studies do not indicate a reduction of the conception rate after the transfer of embryos obtained in vitro and in vivo after fertilization using sorted semen. It remains difficult to justify a significant increase in the frequency of stillbirths of bulls after using sorted sperm. Similarly, 16% of stillbirths of bulls were observed after embryo transfer, when donors were inseminated with sorter semen. The percentage of stillbirths of bulls after embryo transfer with the use of conventional semen is 9%. The sorted semen is not often used for inseminations in pigs, sheep and goats.

Słowa kluczowe

PL

zwierzeta gospodarskie; bydlo; trzoda chlewna; kozy; owce; rozrod zwierzat; wspomaganie rozrodu; inseminacja; nasienie seksowane; wykorzystanie; efektywnosc inseminacji; czynniki ryzyka; technika IVP

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2017
• Tom: 73
• Numer: 05
• Opis fizyczny: s.272-279,tab.,bibliogr.

Twórcy

autor

Jaskowski B.M.

• Instytut Weterynarii, Wydział Medycyny Weterynaryjnej i Nauk o Zwierzętach, Uniwersytet Przyrodniczy w Poznaniu, ul. Wołyńska 28, 60-625 Poznań

autor

Wozna M.

• Instytut Weterynarii, Wydział Medycyny Weterynaryjnej i Nauk o Zwierzętach, Uniwersytet Przyrodniczy w Poznaniu, ul. Wołyńska 28, 60-625 Poznań

autor

Gehrke M.

• Instytut Weterynarii, Wydział Medycyny Weterynaryjnej i Nauk o Zwierzętach, Uniwersytet Przyrodniczy w Poznaniu, ul. Wołyńska 28, 60-625 Poznań

Bibliografia

• An L., Wu Z. H., Wu Y. F., Zhang X. L., Liu X., Zhu Y. B., Cheng W. M., Gao H. M., Guo M., Tian J. H.: Fertility in single-ovulating and superovulated dairy heifers after insemination with low dose sex-sorted sperm. Reprod. Domest. Anim. 2010, 45, 344-350.
• Andersson M., Taponen J., Kommeri M., Dahlbom M.: Pregnancy rates in lactating Holstein-Friesian cows after artificial insemination with sexed sperm. Reprod. Domest. Anim. 2006, 41, 95-97.
• Baruselli P. S., Souza A. H., Martins C. M., Gimenes L. U., Sales J. N. S., Ayres H., Andrade A. F. C., Raphael C. F., Arruda R. P.: Sêmensexado: inseminaçãoartificial e transferência de embriões. Rev. Bras. Reprod. Anim. 2007, 31, 374-381.
• Bathgate R., Grossfeld R., Susetio D., Ruckholdt M., Heasman K., Rath D., Evans G., Maxwell W. M.: Early pregnancy loss in sows after low dose, deep uterine artificial insemination with sex-sorted, frozen-thawed sperm. Anim. Reprod. Sci. 2008, 104, 440-444.
• Bathgate R., Mace N., Heasman K., Evans G., Maxwell W. M., de Graaf S. P.: Birth of kids after artificial insemination with sex-sorted, frozen-thawed goat spermatozoa. Reprod. Domest. Anim. 2013, 48, 893-898.
• Bathgate R., Morton K. M., Eriksson B. M., Rath D., Seig B., Maxwell W. M., Evans G.: Non-surgical deep intra-uterine transfer of in vitro produced porcine embryos derived from sex-sorted frozen-thawed boar sperm. Anim. Reprod. Sci. 2007, 99, 82-92.
• Beilby K. H., Grupen C. G., Thomson P. C., Maxwell W. M., Evans G.: The effect of insemination time and sperm dose on pregnancy rate using sex-sorted ram sperm. Theriogenology 2009, 71, 829-835.
• Bodmer M., Janett F., Hässig M., den Daas N., Reichert P., Thun R.: Fertility in heifers and cows after low dose insemination with sex-sorted and non-sorted sperm under field conditions. Theriogenology 2005, 64, 1647-1655.
• Bombardelli G. D., Soares H. F., Chebel R. C.: Time of insemination relative to reaching activity threshold is associated with pregnancy risk when using sex-sorted semen for lactating Jersey cows. Theriogenology 2016, 85, 533-539.
• Borchersen S., Peacock M.: Danish A. I. field data with sexed semen. Theriogenology 2009, 71, 59-63.
• Butler S. T., Hutchinson I. A., Cromie A. R., Shalloo L.: Applications and cost benefits of sexed semen in pasture-based dairy production systems. Animal 2014, 8, 165-172.
• Cattaneo L., Martin P., Caballero J. O., Calvi M., Vietri B., Puyo E., Medina M.: Pregnancy rate in ewes inseminated with sexed semen in Argentina. Rep. Fert. Dev. 2004, 16, 284-284.
• Cerchiaro I., Cassandro M., Dal Zotto R., Carnier P, Gallo L.: A field study on fertility and purity of sex-sorted cattle sperm. J. Dairy Sci. 2007, 90, 2538-2542.
• Chebel R. C., Guagnini F. S., Santos J. E., Fetrow J. P., Lima J. R.: Sex-sorted semen for dairy heifers: effects on reproductive and lactational performances. J. Dairy Sci. 2010, 93, 2496-2507.
• Cran D. G.: XY sperm separation and use in artificial insemination and other ARTs. Soc. Reprod. Fertil. Suppl. 2007, 65, 475-491.
• Dahlen C., Larson J., Lamb G. C.: Impacts of reproductive technologies on beef production in the United States. Adv. Exp. Med. Biol. 2014, 752, 97-114.
• DeJarnette J. M., McCleary C. R., Leach M. A., Moreno J. F., Nebel R. L., Marshall C. E.: Effects of 2.1 and 3.5 × 10 (6) sex-sorted sperm dosages on conception rates of Holstein cows and heifers. J. Dairy Sci. 2010, 93, 4079-4085.
• DeJarnette J. M., Nebel R. L., Marshall C. E.: Evaluating the success of sex-sorted semen in US dairy herds from on farm records. Theriogenology 2009, 71, 49-58.
• DeJarnette J. M., Nebel R. L., Marshall C. E., Moreno J. F., McCleary C. R., Lenz R. W.: Effect of sex-sorted sperm dosage on conception rates in holstein heifers and Lactating Cows. J. Dairy Sci. 2008, 91, 1778-1785.
• Detterer J., Meinecke-Tillmann S.: Four years practical experience with sexed bull semen in North-West Germany. 27th Annual Meeting A.E.T.E. – Chester, England, 9th-10th September 2011, p. 136.
• Djedović R., Bogdanović V., Stanojević D., Nemes Z., Gáspárdy A., Cseh S.: Involuntary reduction in vigour of calves born from sexed semen. Acta Vet. Hung. 2016, 64, 229-238.
• Dominiquez J. H., Costa D. S., Centurion V. J., Faria F. J.: Pregnancy rate of Nelore females inseminated with male-sexed semen. Anim. Reprod. Sci. 2011, 129, 127-131.
• Espinosa-Cervantes R., Córdova-Izquierdo A.: Sexing sperm of domestic animals. Trop. Anim. Health Prod. 2013, 45, 1-8.
• Frijters A. C., Mullaart E., Roelofs R. M., van Hoorne R. P., Moreno J. F., Moreno O., Merton J. S.: What affects fertility of sexed bull semen more, low sperm dosage or the sorting process? Theriogenology 2009, 71, 64-67.
• Gaviraghi A., Puglisi R., Balduzzi D., Severgnini A., Bornaghi V., Bongioni G., Frana A., Gandini L. M., Lukaj A., Bonacina C., Galli A.: Minimum number of spermatozoa per dose in Mediterranean Italian buffalo (Bubalus bubalis) using sexed frozen semen and conventional artificial insemination. Theriogenology 2013, 79, 1171-1176.
• Grossfeld R., Klinc P., Sieg B., Rath D.: Production of piglets with sexed semen employing a non-surgical insemination technique. Theriogenology 2005, 63, 2269-2277.
• Healy A. A., House J. K., Thomson P. C.: Artificial insemination field data on the use of sexed and conventional semen in nulliparous Holstein heifers. J. Dairy Sci. 2013, 96, 1905-1914.
• Hollinshead F. K., O’Brien J. K., Maxwell W. M., Evans G.: Production of lambs of predetermined sex after the insemination of ewes with low numbers of frozen-thawedsorted X- or Y-chromosome-bearing spermatozoa. Reprod. Fertil. Dev. 2002, 14, 503-508.
• Hutchinson I. A., Shalloo L., Butler S. T.: Expanding the dairy herd in pasture-based systems: the role of sexed semen use in virgin heifers and lactating cows. J. Dairy Sci. 2013, 96, 6742-6752.
• Inaba Y., Abe R., Geshi M., Matoba S., Nagai T., Somfai T.: Sex-sorting of spermatozoa affects developmental competence of in vitro fertilized oocytes in a bull-dependent manner. J. Reprod. Dev. 2016, (in press).
• Jaśkowski J. M., Gehrke M., Żychlińska-Buczek J., Jankowiak T., Gołyś S., Grzelak W., Czeladko J., Ruszkowski J.: Nasienie sortowane – inseminacja krów dawczyń i wyniki pozyskiwania i przenoszenia zarodków. Monografia 2016 (w druku).
• Jaśkowski J. M., Herudzińska M., Kierbić A., Nowak T.: Nasienie seksowane – nowsze dane populacyjne z uwzględnieniem doświadczeń krajowych. Monografia 2016 (w druku).
• Johnson L. A., Rath D., Vazquez J. M., Maxwell W. M., Dobrinsky J. R.: Preselection of sex of offspring in swine for production: current status of the process and its application. Theriogenology 2005, 63,615-624.
• Johnson L. A., Welch.: Sex preselection: high-speed flow cytometric sorting of X and Y sperm for maximum efficiency. Theriogenology1999, 52, 1323-1341.
• Kaimio I., Mikkola M., Lindeberg H., Heikkinen J., Hasler J. F., Taponen J.: Embryo production with sex-sorted semen in superovulated dairy heifers and cows. Theriogenology 2013, 80, 950-954.
• Karakaya E., Yilmazbas-Mecitoglu G., Keskin A., Alkan A., Tasdemir U., Santos J. E., Gumen A.: Fertility in dairy cows after artificial insemination using sex-sorted sperm or conventional semen. Reprod. Domest. Anim. 2014, 49, 333-337.
• Kurykin J., Jaakma U., Jalakas M., Aidnik M., Waldmann A., Majas L.: Pregnancy percentage following deposition of sex-sorted sperm at different sites within the uterus in estrus-synchronized heifers. Theriogenology 2007, 67, 754-759.
• Larson J. E., Lamb G. C., Funnell B. J., Bird S., Martins A., Rodgers J. C.: Embryo production in superovulated Angus cows inseminated four times with sexed-sorted or conventional, frozen-thawed semen. Theriogenology 2010, 73, 698-703.
• Leahy T., Evans G., Maxwell W. M., Marti J. I.: Seminal plasma proteins do not consistently improve fertility after cervical insemination of ewes with non-sorted or sex-sorted frozen-thawed ram spermatozoa. Reprod. Fertil. Dev. 2010, 22, 606-612.
• Lu Y., Liao Y., Zhang M., Yang B., Liang X., Yang X., Lu S., Wu Z., Xu H., Liang Y., Lu K.: A field study on artificial insemination of swamp and crossbred buffaloes with sexed semen from river buffaloes. Theriogenology 2015, 84, 862-867.
• Lucena J. A., Kenyon A. G., Reynolds J. P., Moreno J. F., Lenz R. W., Carroll D., Lehenbauer T. W., Champagne J. D., Aly S. S.: Comparison between low-dose, high-sort and high-dose, low-sort semen on conception and calf sex ratio in Jersey heifers and cows. J. Dairy Sci. 2014, 1782-1789.
• Mallory D. A., Lock S. L., Woods D. C., Poock S. E., Patterson D. J.: Hot topic: Comparison of sex-sorted and conventional semen within a fixed-time artificial insemination protocol designed for dairy heifers. J. Dairy Sci. 2013, 96, 854-856.
• Marinho L. S., Sanches B. V., Rosa C. O., Tannura J. H., Rigo A. G., Basso A. C., Pontes J. H., Seneda M. M.: Pregnancy Rates to Fixed Embryo Transfer of Vitrified IVP Bosindicus, Bostaurus or Bosindicus × Bostaurus Embryos. Reprod. Domest. Anim. 2015, 50, 807-811.
• Mellado M., Coronel F., Estrada A., Ríos F. G.: Fertility in Holstein × Gyr cows in a subtropical environment after insemination with Gyr sex-sorted semen. Trop. Anim. Health Prod. 2010, 42, 1493-1496.
• Mellado M., Sepulveda E., Macias-Cruz U., Avendaño L., Garcia J. E., Veliz F. G., Rodríguez A.: Effects of month of breeding on reproductive efficiency of Holstein cows and heifers inseminated with sex-sorted or conventional semen in a hot environment. Trop. Anim. Health Prod. 2014, 46, 265-269.
• Mikkola M., Andersson M., Taponen J.: Transfer of cattle embryos produced with sex-sorted semen results in impaired pregnancy rate and increased male calf mortality. Theriogenology 2015, 84, 1118-1122.
• Mikulska-Pospiszel A., Tischner M.: Regulacja płci u bydła. Ośmioletnie wyniki inseminacji nasieniem sortowanym (seksowanym) w fermie krów mlecznych. Życie Wet. 2010, 85, 769-771.
• Noonan E. J., Kelly J. C., Beggs D. S.: Factors associated with fertility of nulliparous dairy heifers following a 10-day fixed-time artificial insemination program with sex-sorted and conventional semen. Aust. Vet. J. 2016, 94, 145-148.
• Norman H. D., Miller R. H.: Use of sexed semen and its effect on conception rate, calf sex, dystocia, and stillbirth of Holsteins in the United States. J. Dairy Sci. 2010, 93, 3880-3890.
• Olynk N. J., Wolf C. A.: Expected net present value of pure and mixed sexed semen artificial insemination strategies in dairy heifers. J. Dairy Sci. 2007, 90, 2569-2576.
• Peippo J., Vartia K., Kananen-Anttila K., Räty M., Korhonen K., Hurme T., Myllymäki H., Sairanen A., Mäki-Tanila A.: Embryo production from superovulated Holstein-Friesian dairy heifers and cows after insemination with frozen-thawed sex-sorted X spermatozoa or unsorted semen. Anim. Reprod. Sci. 2009, 111, 80-92.
• Pellegrino C. A., Morotti F., Untura R. M., Pontes J. H., Pellegrino M. F., Campolina J. P., Seneda M. M., Barbosa F. A., Henry M.: Use of sexed sorted semen for fixed-time artificial insemination or fixed-time embryo transfer of in vitro-produced embryos in cattle. Theriogenology 2016, 86, 888-893.
• Presicce G. A., Verberckmoes S., Senatore E. M., Klinc P., Rath D.: First established pregnancies in Mediterranean Italian buffaloes (Bubalusbubalis) following deposition of sexed spermatozoa near the utero tubal junction. Reprod. Domest. Anim. 2006, 41, 95-97.
• Rath D., Barcikowski S., De Graaf S., Garrels W., Grossfeld R., Klein S., Knabe W., Knoor C., Kuels W., Meyer H., Michal J., Noench-Tegeder G., Rehbock C., Taylor U., Washausen S.: Sex selection of sperm in farm animals: status report and developmental prospects. Reproduction 2013, 145, 15-30.
• Rath D., Johnson L. A., Dobrinsky J. R., Welch G. R., Niemann H.: Production of piglets preselected for sex following in vitro fertilization with X and Y chromosome-bearing spermatozoas sorted by flow cytometry. Theriogenology 1997, 47, 795-800.
• Roca J., Parrilla I., Bolarin A., Martinez E. A., Rodriguez-Martinez H.: Will AI in pigs become more efficient? Theriogenology 2016, 86, 187-193.
• SáFilho M. F., Ayres H., Ferreira R. M., Nichi M., Fosado M., Campos Filho E. P., Baruselli P. S.: Strategies to improve pregnancy per insemination using sex-sorted semen in dairy heifers detected in estrus. Theriogenology 2010, 74, 1636-1642.
• SáFilho M. F., Griotto R., Abe E. K., Penteado L., Compos Filho E. P., Moreno J. F., Sala R. V., Nichi M., Baruselli P. S.: Optimizing the use of sex sorted sperm in timed artificial insemination programs for suckled beef cows. J. Anim. Sci. 2012, 90, 1816-1823.
• SáFilho M. F., Nichi M., Soares J. G., Vieira L. M., Melo L. F., Ojeda A., Campos Filho E. P., Gameiro A. H., Sartori R., Baruselli P. S.: Sex-sorted sperm for artificial insemination and embryo transfer programs in cattle. Anim. Reprod. 2014, 11, 217-224.
• Sales J. N., Neves K. A., Souza A. H., Crepaldi G. A., Sala R. V., Fosado M., Campos Filho E. P., de Faria M., SáFilho M. F., Baruselli P. S.: Timing of insemination and fertility in dairy and beef cattle receiving timed artificial insemination using sex-sorted sperm. Theriogenology 2011, 76, 427-435.
• Schenk J. L., Cran D. G., Everett R. W., Seidel G. E. Jr.: Pregnancy rates in heifers and cows with cryopreserved sexed sperm: effects of sperm numbers per inseminate, sorting pressure and sperm storage before sorting. Theriogenology 2009, 71, 717-728.
• Seidel G. E. Jr: Update on sexed semen technology in cattle. Animal 2014, 8, 160-164.
• Seidel G. E. Jr, Allen C. H., JohnsonL A., Holland M. D., Brink Z., Welch G. R., Graham J. E., Cattell M. B.: Uterine horn insemination of heiferswith very low numbers of nonfrozen and sexed. Theriogenology 1997, 48, 1255-1265.
• Seidel G. E. Jr, Schenk J. L., Herickhoff L. A., Doyle S. P., Brink Z., Green R. D., Cran D. G.: Insemination of heifers with sexed sperm. Theriogenology 1999, 52, 1407-1420.
• Soares J. G., Martins C. M., Carvalho N. T., Nicacio A. C., Abreu-Silva A. L., Campos Filho E. P., Torres Jr J. R., SáFilho M. F., Baruselli P. S.: Timing of insemination using sex sorted sperm in embryo production with Bosindicus and Bostaurussuperovulated donors. Anim. Reprod. Sci. 2011, 127, 148-153.
• Thomas J. M., Lock S. L., Poock S. E., Ellersieck M. R., Smith M. F., Patterson D. J.: Delayed insemination of nonestrous cows improves pregnancy rates when using sex-sorted semen in timed artificial insemination of suckled beef cows. J. Anim. Sci. 2014, 92, 1747-1752.
• Trigal B., Gómez E., Caamaño J. N., Muñoz M., Moreno J., Carrocera S., Martín D., Diez C.: In vitro and in vivo quality of bovine embryos in vitro produced with sex-sorted sperm. Theriogenology 2012, 78, 1465-1475.
• Wheeler M. B., Rutledge J. J., Fischer-Brown A., VanEtten T., Malusky S., Beebe D. J.: Application of sexed semen technology to in vitro embryo production in cattle. Theriogenology 2006, 65, 219-227.
• Xu J., Guo Z., Su L., Nedambale T. L., Zhang J., Schenk J., Moreno J. F., Dinnyés A., Ji W., Tian X. C., Yang X., Du F.: Developmental potential of vitrified holstein cattle embryos fertilized in vitro with sex-sorted sperm. J. Dairy Sci. 2006, 89, 2510-2518.
• Xu Z. Z.: Application of liquid semen technology improves conception rate of sex-sorted semen in lactating dairy cows. J. Dairy Sci. 2014, 97, 7298-7304.

Identyfikatory

DOI

10.21521/mw.5696