Simultaneous determination of sucrose and trehalose in olive leaves by spectrophotometry utilizing partial least squares method

• Autorzy: Khanmohammadi M. , Moeini M. , Bagheri Garamarudi A. , Sotudehnia A. , Zarrabi M.
• Języki publikacji: EN

Abstrakty

EN

This study deals with the simultaneous determination of sucrose and trehalose in olive leaf samples, with easy sample preparation step, by second derivative UV–Vis spectrophotometry and partial least squares technique. A training set consisting of 31 binary mixture solutions was applied for the construction of PLS model. The proposed procedure was successfully applied for the simultaneous determination of both carbohydrates in laboratory prepared mixtures and in real samples. The real samples were from different growth stages of olive plant. The root mean square error of prediction was determined to be 1.95 and 2.06 for sucrose and trehalose, respectively. Also, limit of detection was 0.21 ppm for sucrose and 0.26 ppm for trehalose. The proposed procedure is rapid, simple, and easy to perform which can be used for routine analysis of both carbohydrates in plant leaves.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2009
• Tom: 31
• Numer: 4
• Opis fizyczny: p.865-869,fig.,ref.

Twórcy

autor

Khanmohammadi M.

• Chemistry Department, Faculty of Science, IKIU, P.O. Box 34149-1-6818, Qazvin, Iran

autor

Moeini M.

• Chemistry Department, Faculty of Science, IKIU, P.O. Box 34149-1-6818, Qazvin, Iran

autor

Bagheri Garamarudi A.

• Chemistry Department, Faculty of Science, IKIU, P.O. Box 34149-1-6818, Qazvin, Iran
• Department of Chemistry and Polymer Laboratories, Engineering Research Institute, Teheran, Iran

autor

Sotudehnia A.

• Department of Agricultural Biotechnology, Faculty of Engineering, IKIU, Qazvin, Iran

autor

Zarrabi M.

• Department of Agricultural Biotechnology, Faculty of Engineering, IKIU, Qazvin, Iran

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