Expression of bone morphogenetic proteins-2/4 in neural stem cells and their lineages

• Autorzy: Lu H.-Z. , Hu J.-G.
• Języki publikacji: EN

Abstrakty

EN

Neural stem cells (NSCs) are multipotent progenitor cells that possess the ability to self-renew and generate different neural cell types. Bone morphogenetic proteins (BMPs) play critical roles in the determination of the fate of NSCs. Although some reports delineate BMP expression in the early developing central nervous system (CNS), they deal mainly with its expressions in restricted areas. However, little information is available for BMP expression in NSCs and their lineages. In this study, based on an in vitro model in which NSCs can self-renew and differentiate into astrocytes, neurons or oligodendrocytes, we mimicked the environment of NSC development in vivo. Using an antibody which can recognize BMP-2 and BMP-4, we showed that BMP-2/4 is only expressed in astrocytes. Inflammatory cytokines (tumor necrosis factor-α and/or interferon-γ) do not influence the expression pattern of BMPs in NSCs and their lineages. These results suggest that astrocytes might be one source of BMPs during the differentiation of NSCs and in the inflammatory environment after CNS injury.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2009
• Tom: 69
• Numer: 4
• Opis fizyczny: p.441–447,fig.,ref.

Twórcy

autor

Lu H.-Z.

• Central Laboratory, the First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui, People Republic of China

autor

Hu J.-G.

• Central Laboratory, the First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui, People Republic of China

Bibliografia

• Abematsu M, Smith I, Nakashima K (2006) Mechanisms of neural stem cell fate determination: extracellular cues and intracellular programs. Curr Stem Cell Res Ther 1: 267-277.
• Ben-Hur T, Ben-Menachem O, Furer V, Einstein O, Mizrachi- Kol R, Grigoriadis N (2003) Effects of proinflammatory cytokines on the growth, fate, and motility of multipoten­tial neural precursor cells. Mol Cell Neurosci 24: 623­631.
• Chen HL, Panchision DM (2007) Concise review: bone morphogenetic protein pleiotropism in neural stem cells and their derivatives - alternative pathways, convergent signals. Stem Cells 25: 63-68.
• Deleyrolle L, Marchal-Victorion S, Dromard C, Fritz V, Saunier M, Sabourin JC, Tran Van Ba C, Privat A, Hugnot JP (2006) Exogenous and fibroblast growth factor 2/epi- dermal growth factor-regulated endogenous cytokines regulate neural precursor cell growth and differentiation. Stem Cells 24: 748-762.
• Fan L, Young PR, Barone FC, Feuerstein GZ, Smith DH, Mcintosh TK (1996) Experimental brain injury induces differential expression of tumor necrosis factor-alpha mRNA in the CNS. Brain Res Mol Brain Res 36: 287­291.
• Feldhaus B, Dietzel ID, Heumann R, Berger R (2004) Effects of interferon-gamma and tumor necrosis factor- alpha on survival and differentiation of oligodendrocyte progenitors. J Soc Gynecol Investig 11: 89-96.
• Fu SL, Hu JG, Li Y, Wang YX, Jin JQ, Xui XM, Lu PH (2007) A simplified method for generating oligodendro- cyte progenitor cells from neural precursor cells isolated from the E16 rat spinal cord. Acta Neurobiol Exp (Wars) 67: 367-377.
• Fuller ML, DeChant AK, Rothstein B, Caprariello A, Wang R, Hall AK, Miller RH (2007) Bone morphogenetic pro­teins promote gliosis in demyelinating spinal cord lesions. Ann Neurol 62: 288-300.
• Furuta Y, Piston DW, Hogan BL (1997) Bone morphoge­netic proteins (BMPs) as regulators of dorsal forebrain development. Development 124: 2203-2212.
• Giulian D, Chen J, Ingeman J E, George J K, Noponen M (1989) The role of mononuclear phagocytes in wound healing after traumatic injury to adult mammalian brain. J Neurosci 9: 4416-4429.
• Hampton DW, Asher RA, Kondo T, Steeves JD, Ramer MS, Fawcett JW (2007) A potential role for bone morphoge- netic protein signalling in glial cell fate determination following adult central nervous system injury in vivo. Eur J Neurosci 26: 3024-3035.
• Hayashi M, Ueyama T, Nemoto K, Tamaki T, Senba E (2000) Sequential mRNA expression for immediate early genes, cytokines, and neurotrophins in spinal cord injury. J Neurotrauma 17: 203-218.
• Lendahl U, Zimmerman LB, McKay RD (1990) CNS stem cells express a new class of intermediate filament protein. Cell 60: 585-595.
• Liem KF Jr, Tremml G, Roelink H, Jessell TM (1995) Dorsal differentiation of neural plate cells induced by BMP-mediated signals from epidermal ectoderm. Cell 82: 969-979.
• Lu HZ, Wang YX, Li Y, Fu SL, Hang Q, Lu PH (2008) Proliferation and differentiation of oligodendrocyte progeni­tor cells induced from rat embryonic neural precursor cells followed by flow cytometry. Cytometry A 73: 754-760.
• Mabie PC, Mehler MF, Marmur R, Papavasiliou A, Song Q, Kessler JA (1997) Bone morphogenetic proteins induce astroglial differentiation of oligodendroglial-astroglial progenitor cells. J Neurosci 17: 4112-4120.
• Matsuura I, Taniguchi J, Hata K, Saeki N, Yamashita T (2008) BMP inhibition enhances axonal growth and func­tional recovery after spinal cord injury. J Neurochem 105: 1471-1479.
• Mehler MF, Mabie PC, Zhu G, Gokhan S, Kessler JA (2000) Developmental changes in progenitor cell responsiveness to bone morphogenetic proteins differentially modulate progressive CNS lineage fate. Dev Neurosci 22: 74-85.
• Mikawa S, Wang C, Sato K (2006) Bone morphogenetic protein-4 expression in the adult rat brain. J Comp Neurol 499: 613-625.
• Nakamura M, Houghtling RA, MacArthur L, Bayer BM, Bregman BS (2003) Differences in cytokine gene expres­sion profile between acute and secondary injury in adult rat spinal cord. Exp Neurol 184: 313-325.
• Rengachary SS (2002) Bone morphogenetic proteins: basic concepts. Neurosurg Focus 13: 1-6.
• Ricci-Vitiani L, Casalbore P, Petrucci G, Lauretti L, Montano N, Larocca LM, Falchetti ML, Lombardi DG, Gerevini VD, Cenciarelli C, D'Alessandris QG, Fernandez E, De Maria R, Maira G, Peschle C, Parati E, Pallini R (2006) Influence of local environment on the differentiation of neural stem cells engrafted onto the injured spinal cord. Neurol Res 28: 488-492.
• Setoguchi T, Yone K, Matsuoka E, Takenouchi H, Nakashima K, Sakou T, Komiya S, Izumo S (2001) Traumatic injury- induced BMP7 expression in the adult rat spinal cord. Brain Res 921: 219-225.
• Yan P, Li Q, Kim GM, Xu J, Hsu CY, Xu XM (2001) Cellular localization of tumor necrosis factor-alpha fol­lowing acute spinal cord injury in adult rats. J Neurotrauma 18: 563-568.
• Zhang Z, Trautmann K, Artelt M, Burnet M, Schluesener HJ (2006) Bone morphogenetic protein-6 is expressed early by activated astrocytes in lesions of rat traumatic brain injury. Neuroscience 138: 47-53.

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