Enzymatic profile aactivity of grass carp (Ctenopharyngodon idella) after exposure to the pollutant named atrazine (herbicide)

• Autorzy: Khan A. , Yousafzai A.M. , Shah N. , Muhammad , Ahmad M.S. , Farooq M. , Aziz F. , Adnan M. , Rizwan M. , Jawad S.M.
• Języki publikacji: EN

Abstrakty

EN

The present study contemplates the enzymatic profile of grass carp, including lactate dehydrogenase (LDH), creatinine phosphokinases (CPK), serum glutamic-pyruvic transaminase (SGPT), and alkaline phosphatase (Alk Phosp) under atrazine’s acute toxicity effects (LC50) for 01 (15 μl/L), 02 (13 μl/L), 03 (10 μl/L), and 04 (08 μl/L) days/concentration, respectively. For analyzing the enzymatic profile we followed the biochemical analyzer set protocol (Merck micro lab 300 biochemistry analyzer) in the laboratory. Control group concentrations for LDH, CPK, SGPT, and Alk Phosp were 342 IU/ml, 7513.3 IU/ml, 46 mmol/l, and 126.6 IU/ml, respectively. After treatment LDH concentrations were 906, 851, 765, and 545 IU/ml, respectively. CPK concentrations were 1,737, 2,445, 3,718, and 5,767 IU/ml, respectively. SGPT concentrations were 27, 24.3, 13.67, and 8.67, respectively, and Alk Phosp concentrations were 50.3, 30, 22.3, and 17.6 IU/ml, respectively. Maximum inclined (P≤0.001) in concentration of LDH was observed after 24 hrs exposure because of hepatic tissue damage, resulting in increased membrane permeability causing enhanced leaching out of LDH and as LDH participates in an anaerobic pathway, so increase LDH mean increases of anaerobic metabolism resulting from depletion of energy under environmental stress conditions by atrazine, while other enzymatic components like CPK, SGPT, and Alk Phosp showed kindred attributes in their result, like all parameter concentrations showed perpetual decline (P≤0.001) in their concentrations indicating reduced enzymatic activity due to a reduction in permeability for these enzymes, forcing the enzymes to accumulate in the cells as well as decrease in enzyme synthesis due to intoxication of atrazine.

• Wydawca: -
• Czasopismo: Polish Journal of Environmental Studies
• Rocznik: 2016
• Tom: 25
• Numer: 5
• Opis fizyczny: p.2003-2008,fig.,ref.

Twórcy

autor

Khan A.

• Department of Zoology, University of Swabi, Khyber Pakhtunkhwa, Pakistan

autor

Yousafzai A.M.

• Department of Zoology, Islamia College Peshawar, Khyber Pakhtunkhwa, Pakistan

autor

Shah N.

• Department of Zoology, University of Swabi, Khyber Pakhtunkhwa, Pakistan

autor

Muhammad

• Department of Microbiology, University of Swabi, Khyber Pakhtunkhwa, Pakistan

autor

Ahmad M.S.

• Department of Zoology, University of Swabi, Khyber Pakhtunkhwa, Pakistan

autor

Farooq M.

• Department of Microbiology, University of Swabi, Khyber Pakhtunkhwa, Pakistan

autor

Aziz F.

• Department of Zoology, University of Swabi, Khyber Pakhtunkhwa, Pakistan

autor

Adnan M.

• Department of Agriculture, University of Swabi, Khyber Pakhtunkhwa, Pakistan

autor

Rizwan M.

• Center of Biotechnology and Microbiology, University of Peshawar, Khyber Pakhtunkhwa, Pakistan

autor

Jawad S.M.

• Department of Zoology, Islamia College Peshawar, Khyber Pakhtunkhwa, Pakistan

Bibliografia

• 1. JOHN P.J. Alteration of certain blood parameters of freshwater teleost Mystus vittatus after chronic exposure to Metasystox and Sevin. Fish Physiology and Biochemistry. 33, 15, 2007.
• 2. VELCHEVA I., PETROVA S., MOLLOV I., GECHEVA G., GEORGIEV D. Herbicides impact on the community structure of the soil mesofauna. Bulgarian Journal of Agricultural Science. 18 (5), 742, 2012.
• 3. MARIN-MORALES M.A., VENTURA-CAMARGO B.D.C, HOSHINA M.M. Toxicity of Herbicides: Impact on aquatic and soil biota and human health. In: A. Price and J.A. Kelton (Eds) Herbicides – Current Research and Case Studies in Use, In Tech, Croatia, 399, 2013.
• 4. LUSKOVA V., SVOBODA M., KOLAROVA J. The effect of diazinon on blood plasma biochemistry in Carp (Cprinus carpio). Acta Vet. Brno.71, 117, 2002.
• 5. NELSON D.L., COX M.M. Lehninger, Principles of Biochemistry 3rd Ed. Worth Publishing: New York. ISBN 1-57259-153-6. 2000.
• 6. WANG C.S., CHANG TING-TSUNG, YAO, WEIJEN, WANG, SHAN-TAIR, CHOU, PESUS. Impact of increasing alanine aminotransferase levels within normal range on incident diabetes. J. Formos. Med. Assoc. 111, 201, doi:10.1016/j.jfma.2011.04.004. 2012.
• 7. GHOURI N., PREISS D., SATTAR, NAVEED. Liver enzymes, nonalcoholic fatty liver disease, and incident cardiovascular disease: a narrative review and clinical perspective of prospective data". Hepatology. 52, 1156, 2010.
• 8. DONG X., ZHU L., WANG J., XIE H., HOU X., JIA W. Effects of atrazine on cytochrome P450 enzymes of zebrafish (Danio rerio). Chemosphere 77, 404, 2009.
• 9. KREUTZ L.C., BARCELLOS L.J.G., MARTENINGHE A., DOS S., ZANATTA E.D. Innate immune response of silver catfish (Rhamdia quelen) exposed to atrazine. Fish Shellfish Immunology. 33, 1055, 2012.
• 10. BRUN-HEATH I., TAILLANDIER A., SERRE J.L., MORNET E. Characterization of 11 novel mutations in the tissue non-specific alkaline phosphatase gene responsible for hypophosphatasia and genotype-phenotype correlations. Mol. Genet. Metab. 84, 273-73, 2005.
• 11. NASCIMENTO C.R.B., SOUZA M.M., MARTINEZ C.B.R. Copper and the herbicide atrazine impair the stress response of the freshwater fish, Prochilodus lineatus. Pharmacology Toxicological Endocrinology. 155, 456., 2012.
• 12. NWANI C.D., LAKRA W.S., NAGPURE N.S., KUMAR R., KUSHWAHA B., KUMAR S.K. Toxicity of the herbicide atrazine: effects on lipid peroxidation and activities of antioxidant enzymes in the freshwater fish, Channa punctatus (Bloch). International Journal of Environmental Research and Public Health. 7, 3298, 2010.
• 13. RYMUSZKA A., SIWICKI A.K., SIEROSLA W.A. Determination of modulatory potential of atrazine on selected functions of immune cells isolated from rainbow trout (Oncorhynchus mykiss). Central European Journal of Immunology. 32, 97, 2007.
• 14. TIERNEY K.B., SINGH C.R., ROSS P.S., KENNEDY C.J. Relating olfactory neurotoxicity to altered olfactorymediated behaviors in rainbow trout exposed to three currently-used pesticides. Aquatic Toxicology. 81, 55, 2007.
• 15. FATIMA M., MANDIKI S.N.M., DOUXFILS J., SILVESTRE F., COPPE P., KESTEMONT P. Combined effects of herbicides on biomarkers reflecting immuneendocrine interactions in goldfish immune and antioxidant effects. Aquatic Toxicology. 81, 159, 2007.
• 16. LIONETTO M.G., CARICATO R., GIORDANO M.E., ERROI E., SCHETTINO T. Carbonic anhydrase as pollution biomarker: an ancient enzyme with a new use. International Journal of Environmental Research and Public Health. 9, 3965, 2012.
• 17. TILLITT D.E., PAPOULIAS D.M., WHYTE J.J., RICHTER C.A. Atrazine reduces reproduction in fathead minnow (Pimephales promelas). Aquatic Toxicology. 99, 149, 2010.
• 18. NELSON D.L., COX M.M. Lehninger Principles of Biochemistry. 4th ed.; WH Freeman and Co. New York. 1013, 2004.
• 19. GUYTON A.C., HALL, JOHN E. Guyton and Hall Textbook of Medical Physiology with Student Consult Online Access (11th ed.). Philadelphia: Elsevier Saunders. ISBN 0-7216-0240-1, 2006.
• 20. ZIKIC R.V., STAJN S., PAVLOVIC Z., OGNJANOVIC B.I., SAICIC Z.S. Activities of superoxide dismutase and catalase in erytrocyte and plasma transaminases of goldfish (Carassius auratus gibelio Bloch.) exposed to cadmium. Physiological Research. 50, 105. 2001.
• 21. DATTA S., SAHA D.R., GOSH D., MAJUMDAR T., BHATTACHARYA S., MAZUMDER S. Sub-lethal concentration of arsenic interferes with the proliferation of hepatocytes and induces in vivo apoptosis in Clarias batrachus L. Comparative Biochemistry and Physiology. 145, 339, 2007.
• 22. ROY S., BHATTACHARYA S. Arsenic-induced histopathology and synthesis of stress proteins in liver of Channa punctatus. Ecotoxicological and Environmental Safety. 65, 218, 2006.
• 23. BANAEE M., SUREDA A., MIRVAGHEFI A.R., AHMADI K. Effects of Diazinon on Biochemical Parameters of Blood in Rainbow Trout (Oncorhynchus mykiss). Pesticide Biochemistry and Physiology. 99, 1, 2012.
• 24. BANAEE M., SUREDA A., MIRVAGHEFI A.R., AHMADI K. Biochemical and histological changes in the liver tissue of Rainbow trout (Oncorhynchus mykiss) exposed to sublethal concentrations of diazinon. Fish Physiology and Biochemistry. 39, 489, 2013.
• 25. DOUBEK J., SLOSARKOVA S., REHAKOVA K., BOUDA J., SCHEER P., PIPERISOVA I., TOMENENDALOVA J., MATALOVA E. The interpretation of basic biochemical and hematological indices in animals. Noviko Czech Republic, 102, 2010 [In Czech].
• 26. BANAEE M., AHMADI K. Sub-lethal Toxicity Impacts of Endosulfan on some biochemical Parameters of the Freshwater Crayfish (Astacus leptodactylus). Research Journal of Environmental Sciences. 5 (11), 827, 2011.
• 27. RAO J.V. Biochemical alterations in euryhaline fish, Oreochromis mossambicus, exposed to sub-lethal concentrations of an organophosphorus insecticide, monocrotophos. Chemosphere. 65, 1814, 2006.
• 28. YOUSAFZAI A.M., A.R SHAKOORI. Hepatic responses of a freshwater fi sh against aquatic pollution. Pakistan Journal of Zoology. 43 (2), 209, 2011.
• 29. LAVANYA S., RAMESH M., KAVITHA C., MALARVIZHI A. Hematological, biochemical and ionoregulatory responses of Indian major carp, Catla catla during chronic sublethal exposure to inorganic arsenic. Chemosphere. 82 (7), 977, 2011.
• 30. LI Z H., VELISEK J., GRABIC R., LI P., KOLAROVA J., RANDAK T. Use of hematological and plasma biochemical parameters to assess the chronic effects of a fungicide propiconazole on a freshwater teleost. Chemosphere, 83 (4), 572, 2011.
• 31. SARAVANAN M., KARTHIKA S., MALARVIZHI A.M., RAMESH. Ecotoxicological impacts of clofi bric acid and diclofenac in common carp (Cyprinus carpio) fingerlings: hematological, biochemical, ionoregulatory and enzymological responses. Journal of Hazardous Materials. 195, 188, 2011.
• 32. TILAK K.S., VEERAIAH K., LAKSHMI S.J. Studies of some biochemical changes in the tissues of Catla catla (Hamilton), Labeo rohita (Hamilton) and Cirrhinus mrigala (Hamilton) exposed to NH3-N, NO2- N and NO3-N. Journal of environmental biology 23, 377, 2002.
• 33. DAS B.K., MUKHERJEE S.C. Toxicity of cypermethrin in Labeo rohita fingerlings: biochemical, enzymatic and haematological consequences. Comparative Biochemistry and Physiology. Toxicology and Pharmacology. 134, 109, 2003.
• 34. VELISEK J.Z., SVOBODOVA V., PIACKOVA L., NOVOTNY J., BLAHOVA E., SUDOVA, MALY V. Effects of metribuzin on rainbow trout Oncorhynchus mykiss. Veterinarni Medicina. 53, 324. 2008.
• 35. BANAEE M., MIRVAGEFEI A.R., RAFEI G.R., MAJAZI A.B. Effect of sub-lethal Diazinon Concentrations on Blood Plasma Biochemistry. International Journal of Environmental Research. 2, 189, 2008.

Identyfikatory

DOI

10.15244/pjoes/62821