CTLA-4 polymorphisms (+49 A/G and -318 C/T) are important genetic determinants of AITD susceptibility and predisposition to high levels of thyroid autoantibodies in Polish children - preliminary study

• Autorzy: Pastuszak-Lewandoska D. , Domanska D. , Rudzinska M. , Bossowski A. , Kucharska A. , Sewerynek E. , Czarnecka K. , Migdalska-Sek M. , Czarnocka B.
• Języki publikacji: EN

Abstrakty

EN

Autoimmune thyroid diseases (AITDs), including Hashimoto' s thyroiditis (HT) and Graves' disease (GD), are related to environmental and genetic factors. We analyzed the association of cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4) gene two polymorphisms (+49 A/G, -318 C/T) with HT and GD development in Polish children, and correlated both polymorphisms with the production of thyroid autoantibodies (TPOAb and TgAb). The study involved 49 AITD patients (age 10-19) with HT (n=25) or GD (n=24) and 69 healthy controls. SNP genotyping was performed using genomic DNA and TaqMan® probes. The obtained results indicated that CTLA-4 +49 GG genotype was significantly more frequent in both HT and GD patients, whereas the AA genotype was more common in controls. CTLA-4-318 CT genotype was significantly more frequent in AITD, and the CC genotype more often occurred in controls. Significantly higher median TPOAb and TgAb values were associated with G allele in HT, and with T allele in GD patients. Concluding, both studied polymorphisms seem to be important genetic determinants of the risk of HT and GD, and appear to be associated with a predisposition to high levels of TAbs and clinical AITD. The obtained results give more information on the distribution of the CTLA-4 polymorphism in Polish AITD children, and further support the proposal that the CTLA-4 gene plays an important role in a TAb production.

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2013
• Tom: 60
• Numer: 4
• Opis fizyczny: p.641-646,ref.

Twórcy

autor

Pastuszak-Lewandoska D.

• Department of Molecular Bases of Medicine, 1st Chair of Internal Diseases, Medical University of Lodz, Lodz, Poland

autor

Domanska D.

• Department of Molecular Bases of Medicine, 1st Chair of Internal Diseases, Medical University of Lodz, Lodz, Poland

autor

Rudzinska M.

• Department of Biochemistry & Molecular Biology, Medical Center of Postgraduate Education, Warsaw, Poland

autor

Bossowski A.

• Department of Pediatrics, Endocrinology, Diabetology with Cardiology Division, Medical University, Bialystok, Bialystok, Poland

autor

Kucharska A.

• Department of Pediatrics and Endocrinology, Medical University of Warsaw, Warsaw, Poland

autor

Sewerynek E.

• Department of Endocrine Disorders and Bone Metabolism, 1st Chair of Endocrinology, Medical University of Lodz, Lodz, Poland

autor

Czarnecka K.

• Department of Molecular Bases of Medicine, 1st Chair of Internal Diseases, Medical University of Lodz, Lodz, Poland

autor

Migdalska-Sek M.

• Department of Molecular Bases of Medicine, 1st Chair of Internal Diseases, Medical University of Lodz, Lodz, Poland

autor

Czarnocka B.

• Department of Molecular Bases of Medicine, 1st Chair of Internal Diseases, Medical University of Lodz, Lodz, Poland

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