Helicobacter pylori isolation, serology and cagA, cagE and virB11 detection in patients with non-ulcer dyspepsia from Turkey: Correlation with histopathologic findings

• Autorzy: Ugur Ilga , Mustafa Ozyurt , Sukru Yildirim , Koray Ergunay , Nurittin Ardic , Levent Demirturk , Tuncer Haznedaroglu
• Języki publikacji: EN

Abstrakty

EN

Colonization with Helicobacter pylori (HP) may have major clinical consequences and HP virulence factors are associated with more severe gastroduodenal pathologies. In this study, prevalence of HP in patients with Non-Ulcer Dyspepsia (NUD) was determined by rapid urease test and culture and correlations of histopathologic changes with bacterial virulence factors and serologic profiles were investigated. Gastric biopsies from sixty-nine patients admitted to Haydarpasa Training Hospital Department of Gastroenterology were evaluated for rapid urease, HP isolation and examined histopathologically. PCR was employed for HP confirmation and detection of HP cagA, cagE and virB11 genes. For each patient, IgG and IgA antibodies and anti-cagA antibodies were also determined by ELISA tests. HP was isolated and confirmed by PCR in 74% (51/69) of the patients. Anti-HP IgG and IgA were detected in 96% (49/51) and 53% (27/51), respectively. Anti-cagA were present in 51% (26/51). cagA, cagE and virB11 were positive in 56.8% (29/51), 60.7% (31/51) and 58.8% (30/51) of the patients, respectively. Statistically significant correlation was observed between cagA PCR and inflammation/activity scores. Detection of cagA by molecular assays can be an alternative test that can be employed for individual patient assessment.

Słowa kluczowe

EN

Helicobacter pylori; Serology; Pathogenesis; Non-ulcer dyspepsia; CagA

• Czasopismo: Open Medicine
• Rocznik: 2008
• Tom: 3
• Numer: 1
• Strony: 41-46

Daty

wydano

2008-03-01

online

2008-03-01

Twórcy

autor

Ugur Ilga

• Haydarpasa Training Hospital, Department of Microbiology and Clinical Microbiology, Gulhane Military Medical Academy, Istanbul, Turkey

autor

Mustafa Ozyurt

• Haydarpasa Training Hospital, Department of Microbiology and Clinical Microbiology, Gulhane Military Medical Academy, Istanbul, Turkey,

autor

Sukru Yildirim

• Haydarpasa Training Hospital, Department of Pathology, Gulhane Military Medical Academy, Istanbul, Turkey

autor

Koray Ergunay

• Faculty of Medicine, Department of Clinical Microbiology, Hacettepe University, Ankara, Turkey

autor

Nurittin Ardic

• Haydarpasa Training Hospital, Department of Microbiology and Clinical Microbiology, Gulhane Military Medical Academy, Istanbul, Turkey

autor

Levent Demirturk

• Haydarpasa Training Hospital, Department of Gastroenterology, Gulhane Military Medical Academy, Istanbul, Turkey

autor

Tuncer Haznedaroglu

• Haydarpasa Training Hospital, Department of Microbiology and Clinical Microbiology, Gulhane Military Medical Academy, Istanbul, Turkey

Bibliografia

• [1] Fox J.G., The non-H. pylori helicobacters: their expanding role in gastrointestinal and systemic diseases. Gut., 2002, 50, 273–283 http://dx.doi.org/10.1136/gut.50.2.273[Crossref]
• [2] Kusters J.G., Gerrits M.M., Van Strijp J.A., Vandenbroucke-Grauls C.M., Coccoid forms of Helicobacter pylori are the morphologic manifestation of cell death. Infect. Immun., 1997, 65, 3672–3679
• [3] O’Toole P.W., Lane M.C., Porwollik S., Helicobacter pylori motility. Microbes Infect., 2000, 2, 1207–1214 http://dx.doi.org/10.1016/S1286-4579(00)01274-0[Crossref]
• [4] Ernst P.B., Gold B.G., The disease spectrum of Helicobacter pylori: the immunopathogenesis of gastroduodenal ulcer and gastric cancer. Annu. Rev. Microbiol., 2000, 54, 615–640 http://dx.doi.org/10.1146/annurev.micro.54.1.615[Crossref]
• [5] Kusters J.G., van Vliet A.H.M., Kuipers E.J., Pathogenesis of Helicobacter pylori Infection. Clin. Microbiol. Rev., 2006, 19, 449–490 http://dx.doi.org/10.1128/CMR.00054-05[Crossref]
• [6] Perez-Perez G.I., Rothenbacher D., Brenner H., Epidemiology of Helicobacter pylori infection. Helicobacter, 2004, 9(1), 1–6 http://dx.doi.org/10.1111/j.1083-4389.2004.00248.x[Crossref]
• [7] Megraud F., Lehours P., Helicobacter pylori Detection and Antimicrobial Susceptibility Testing. Clin. Microbiol. Rev., 2007, 20, 280–322 http://dx.doi.org/10.1128/CMR.00033-06[Crossref]
• [8] Dixon M.F., Path F.R.C., Genta R.M., Yardley J.H., Correa P., Classification and Grading of Gastritis: the Updated Sydney System. Am. J. Surg. Pathol., 1996, 20, 1161–1181 http://dx.doi.org/10.1097/00000478-199610000-00001[Crossref]
• [9] Tomasini M.L., Zanussi S., Sozzi M., Tedeschi R., Basaglia G., Paoli P., Heterogeniety of cag genotypes in Helicobacter pylori isolates from human biopsy specimens. J. Clin. Microbiol., 2003, 41, 976–980 http://dx.doi.org/10.1128/JCM.41.3.976-980.2003[Crossref]
• [10] Parsonnet J., Helicobacter pylori. Infect. Dis. Clin. N. Am., 1998, 12, 185–197 http://dx.doi.org/10.1016/S0891-5520(05)70417-7[Crossref]
• [11] Taylor D.N., Blaser M.J., The epidemiology of Helicobacter pylori infection. Epidemiol. Rev., 1991, 13, 42–59
• [12] Pounder R.E., Nge D., The prevalence of Helicobacter pylori infection in different countries. Aliment. Pharmacol. Ther., 1995, 9, 33–39
• [13] Malaty H.M., Graham D.Y., Importance of childhood socioeconomic status on the current prevalence of Helicobacter pylori infection. Gut, 1994, 35, 742–745 http://dx.doi.org/10.1136/gut.35.6.742[Crossref]
• [14] Oguz D., Eskioglu E., Koseoglu T., Unver E., Terzioglu S., Kulacoglu S., Ust gastrointestinal sistem hastaliklarinda Helicobacter pylori. Turk. Gastroenterol. Derg., 1995, 6, 440–446, (in Turkish)
• [15] Ozden A., Midenizdeki yabanci. 1st ed. Turk Gastroenteroloji Vakfi yayinlari, Ankara, 2003 (in Turkish)
• [16] Perez-Perez G.I., Dworkin B.M., Chodos J.E., Blaser M.J., Campylobacter pylori antibodies in humans. Ann. Intern. Med., 1988, 109, 11–17 [Crossref]
• [17] Mattsson A., Quiding-Jarbrink M., Lonroth H., Hamlet A., Ahlstedt I., Svennerholm A., Antibody-secreting cells in the stomachs of symptomatic and asymptomatic Helicobacter pylori-infected subjects. Infect. Immun., 1988, 66, 2705–2712
• [18] Nurgalieva Z.Z., Conner M.E., Opekun A.R., Zheng C.Q., Elliott S.N., Ernst P.B., et al., B-cell and T-cell immune responses to experimental Helicobacter pylori infection in humans. Infect. Immun., 2005, 73, 2999–3006 http://dx.doi.org/10.1128/IAI.73.5.2999-3006.2005[Crossref]
• [19] Karakas A., Polat H., Aksoy F., Vatansev C., Endoskopi yapilan olgularda Helicobacter pylori prevalansi, tani metotlari ve hastaliklarla olan iliskisi. MN Klinik Bilimler & Doktor, 2002, 8, 5–12, (in Turkish)
• [20] Kuipers E.J., Perez-Perez G.I., Meuwissen S.G., Blaser M.J., Helicobacter pylori and atrophic gastritis: importance of the cagA status. J. Natl. Cancer. Inst., 1995, 87, 1777–1780 http://dx.doi.org/10.1093/jnci/87.23.1777[Crossref]
• [21] Parsonnet J., Friedman G.D., Orentreich N., Vogelman H., Risk for gastric cancer in people with CagA positive or CagA negative Helicobacter pylori infection. Gut, 1997, 40, 297–301 [Crossref]
• [22] Nomura M., Lee J., Stemmermann G.N., Nomura R.Y., Perez-Perez G.I., Blaser M.J., Helicobacter pylori CagA seropositivity and gastric carcinoma risk in a Japanese American population. J. Infect. Dis., 2002, 186, 1138–1144 http://dx.doi.org/10.1086/343808[Crossref]
• [23] Hayashi S., Sugiyama T., Yokota K., Isogai H., Isogai E., Oguma K., et al., Analysis of immunoglobulin A antibodies to Helicobacter pylori in serum and gastric juice in relation to mucosal inflammation. Clin. Diagn. Lab. Immunol., 1998, 5, 617–621
• [24] Sommer F., Faller G., Rollinghoff M., Kirchner T., Mak T.W., Lohoff M., Lack of gastritis and of an adaptive immune response in interferon regulator factor-1-deficient mice infected with Helicobacter pylori. Eur. J. Immunol., 2001, 31, 396–402 http://dx.doi.org/10.1002/1521-4141(200102)31:2<396::AID-IMMU396>3.0.CO;2-Y[Crossref]
• [25] Podzorski R.P., Podzorski D.S., Wueth A., Tolia V., Analysis of the vacA, cagA, cagE, iceA, and babA2 genes in Helicobacter pylori from sixty-one pediatric patients from the Midwestern United States. Diagn Microbiol Infect Dis., 2003, 46, 83–88 http://dx.doi.org/10.1016/S0732-8893(03)00034-8[Crossref]
• [26] Shimoyama T., Fukuda S., Tanaka M., Mikami T., Saito Y., Munakata A., High prevalence of the CagApositive Helicobacter pylori strains in Japanese asymptomatic patients and gastric cancer patients. Scand. J. Gastroenterol., 1997, 32, 465–468 http://dx.doi.org/10.3109/00365529709025082[Crossref]
• [27] Shyu R.Y., Jiang S.Y., Lai C.H., Hsu C.T., Young T.H., Yeh M.Y., High frequency of cytotoxin-associated gene A in Helicobacter pylori isolated from asymptomatic subjects and peptic ulcer patients in Taiwan. J. Clin. Gastroenterol., 1998, 27, 54–59 http://dx.doi.org/10.1097/00004836-199807000-00011[Crossref]
• [28] Erzin Y., Altun S., Dobrucali A., Aslan M., Erdamar S., Dirican A., et al., Analysis of serum antibody profile against H pylori VacA and CagA antigens in Turkish patients with duodenal ulcer. World. J. Gastroenterol., 2006, 12, 6869–6873
• [29] Sezikli M., Guliter S., Apan T.Z., Aksoy A., Keles H., Ozkurt Z.N., Frequencies of serum antibodies to Helicobacter pylori CagA and VacA in a Turkish population with various gastroduodenal diseases. Int. J. Clin. Pract., 2006, 60, 1239–1243 http://dx.doi.org/10.1111/j.1742-1241.2005.00778.x[Crossref]
• [30] Demirturk L., Ozel A.M., Yazgan Y., Solmazgul E., Yildirim S., Gultepe M. et al., CagA status in dyspeptic patients with and without peptic ulcer disease in Turkey: association with histopathologic findings. Helicobacter, 2001, 6, 163–168 http://dx.doi.org/10.1046/j.1523-5378.2001.00024.x[Crossref]
• [31] Maeda S., Yoshida H., Ikenoue T., Ogura K., Kanai F., Kato N., et al., Structure of cag pathogenicity island in Japanese Helicobacter pylori isolates. Gut, 1999, 44, 336–341 http://dx.doi.org/10.1136/gut.44.3.336[Crossref]
• [32] van Doorn L.J., Figueiredo C., Sanna R., Blaser M.J., Quint W.G., Distinct variants of Helicobacter pylori cagA are associated with vacA subtypes. J. Clin. Microbiol., 1999, 37, 2306–2311

Identyfikatory

DOI

10.2478/s11536-007-0062-y