Identifying the etiologic role of Parvovirus B19 in non-immune hydrops fetalis by histopathology, immunohistochemistry and nucleic acid testing: a retrospective study

• Autorzy: Koray Ergunay , Gulcin Altinok , Bora Gurel , Ahmet Pinar , Arzu Sungur , Sevim Balci , Semsettin Ustacelebi
• Języki publikacji: EN

Abstrakty

EN

Intrauterine Parvovirus B19 infections may cause fetal anemia, non-immune hydrops fetalis or abortion. This study focuses on the pathogenic role of Parvovirus B19 in non-immune hydrops fetalis at Hacettepe University, a major reference hospital in Turkey. Twenty-two cases of non-immune hydrops fetalis were retrospectively selected out of a total of 431 hydrops fetalis specimens from the Department of Pathology archieves. Paraffine embedded tissue sections from placental and liver tissues from each case were evaluated by histopathology, immunohistochemistry, nested PCR and commercial quantitative Real-time PCR. Viral DNA was detected in placental tissues by Real-time PCR in 2 cases (2/22, 9.1%) where histopathology also revealed changes suggestive of Parvovirus B19 infection. No significant histopathologic changes were observed for the remaining sections. Nested PCR that targets the VP1 region of the viral genome and immunohistochemistry for viral capsid antigens were negative for all cases. As a result, Parvovirus B19 is identified as the etiologic agent for the development of non-immune hydrops fetalis for 9.1% of the cases in Hacettepe University, Turkey. Real-time PCR is observed to be an effective diagnostic tool for nucleic acid detection from paraffine embedded tissues. Part of this study was presented as a poster at XIIIth International Congress of Virology, San Francisco, USA (Abstract V-572).

Słowa kluczowe

EN

Hydrops fetalis; non-immune hydrops fetalis; Parvovirus B19; immunohistochemistry; nested PCR; Real-time PCR

• Czasopismo: Open Medicine
• Rocznik: 2007
• Tom: 2
• Numer: 3
• Strony: 271-279

Daty

wydano

2007-09-01

online

2007-06-09

Twórcy

autor

Koray Ergunay

• Faculty of Medicine, Department of Clinical Microbiology, Hacettepe University, 06100, Ankara, Turkey,

autor

Gulcin Altinok

• Faculty of Medicine, Department of Pathology, Hacettepe University, 06100, Ankara, Turkey

autor

Bora Gurel

• Faculty of Medicine, Department of Pathology, Hacettepe University, 06100, Ankara, Turkey

autor

Ahmet Pinar

• Faculty of Medicine, Department of Clinical Microbiology, Hacettepe University, 06100, Ankara, Turkey

autor

Arzu Sungur

• Faculty of Medicine, Department of Pathology, Hacettepe University, 06100, Ankara, Turkey

autor

Sevim Balci

• Faculty of Medicine, Department of Pediatrics, Division of Clinical Genetics, Hacettepe University, 06100, Ankara, Turkey

autor

Semsettin Ustacelebi

• Faculty of Medicine, Department of Clinical Microbiology, Hacettepe University, 06100, Ankara, Turkey

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Identyfikatory

DOI

10.2478/s11536-007-0029-z