The computational modelling of the kinetics of ascorbic acid palmitate hydrolysis by lipase considering diffusion

• Autorzy: Jurgita Dabulytė-Bagdonavičienė , Feliksas Ivanauskas , Valdemaras Razumas
• Języki publikacji: EN

Abstrakty

EN

This paper presents mathematical and computational modelling of kinetics of a bioelectroanalytical system based on the interfacial action of hydrolytic enzyme. A system of non-linear differential equations with diffusion is used to describe the kinetics of Termomyces lanuginosa lipase (TLL) catalyzed hydrolysis of L-ascorbic acid palmitate (AAP). The system was solved numerically, and the kinetic prameters of AAP hydrolysis by the enzyme were determined. The experimental and modelling results show linear dependence of the rate of AAP hydrolysis on the TLL concentration. Complex dependence of the initial rate of bioelectrocatalytic current increase on the thickness of total diffusion layer (hydrodynamic diffusion layer plus thickness of dialysis membrane on the electrode surface) is also demonstrated and explained. [...]

Słowa kluczowe

EN

Ascorbic acid; Lipase; Enzyme reaction; Mathematical model; Computational model

• Czasopismo: Open Chemistry
• Rocznik: 2011
• Tom: 9
• Numer: 4
• Strony: 712-719

Daty

wydano

2011-08-01

online

2011-06-04

Twórcy

autor

Jurgita Dabulytė-Bagdonavičienė,

autor

Feliksas Ivanauskas

• Department of Computer Science, Vilnius University, LT-03225, Vilnius, Lithuania

autor

Valdemaras Razumas

• Institute of Biochemistry, Vilnius University, LT-08662, Vilnius, Lithuania

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Identyfikatory

DOI

10.2478/s11532-011-0053-3