Formation of amyloid-like aggregates through the attachment of protein molecules to a Congo red scaffolding framework ordered under the influence of an electric field

• Autorzy: Barbara Stopa , Barbara Piekarska , Leszek Konieczny , Marcin Król , Janina Rybarska , Anna Jagusiak , Paweł Spólnik , Irena Roterman , Barbara Urbanowicz , Piotr Piwowar , Krzysztof Lewiński
• Języki publikacji: EN

Abstrakty

EN

This study describes a technique which makes it possible to introduce the amyloid-like order to protein aggregates by using the scaffolding framework built from supramolecular, fibrillar Congo red structures arranged in an electric field. The electric field was used not only to obtain a uniform orientation of the charged dye fibrils, but also to make the fibrils long, compact and rigid due to the delocalization of pi electrons, which favors ring stacking and, as a consequence, results in an increased tendency to self-assemble. The protein molecules (immunoglobulin L chain lambda, ferritin) attached to this easily adsorbing dye framework assume its ordered structure. The complex precipitating as plate-like fragments shows birefringence in polarized light. The parallel organization of fibrils can be observed with an electron microscope. The dye framework may be removed via reduction with sodium dithionite, leaving the aggregated protein molecules in the ordered state, as confirmed by X-ray diffraction studies. [...]

Słowa kluczowe

EN

Congo red; Amyloid formation; Electric field

• Czasopismo: Open Chemistry
• Rocznik: 2010
• Tom: 8
• Numer: 1
• Strony: 41-50

Daty

wydano

2010-02-01

online

2010-02-16

Twórcy

autor

Barbara Stopa

• Chair of Medical Biochemistry, Jagiellonian University Medical College, 31-034, Kraków, Poland,

autor

Barbara Piekarska

• Chair of Medical Biochemistry, Jagiellonian University Medical College, 31-034, Kraków, Poland

autor

Leszek Konieczny

• Chair of Medical Biochemistry, Jagiellonian University Medical College, 31-034, Kraków, Poland

autor

Marcin Król

• Department of Bioinformatics and Telemedicine, Jagiellonian University Medical College, 31-530, Kraków, Poland

autor

Janina Rybarska

• Chair of Medical Biochemistry, Jagiellonian University Medical College, 31-034, Kraków, Poland

autor

Anna Jagusiak

• Chair of Medical Biochemistry, Jagiellonian University Medical College, 31-034, Kraków, Poland

autor

Paweł Spólnik

• Chair of Medical Biochemistry, Jagiellonian University Medical College, 31-034, Kraków, Poland

autor

Irena Roterman

• Department of Bioinformatics and Telemedicine, Jagiellonian University Medical College, 31-530, Kraków, Poland

autor

Barbara Urbanowicz

• Electron Microscopy Laboratory, University Children’s Hospital, 30-663, Kraków, Poland

autor

Piotr Piwowar

• Department of Measurements and Instrumentation, AGH-University of Science and Technology, 30-059, Kraków, Poland

autor

Krzysztof Lewiński

• Faculty of Chemistry, Jagiellonian University, 30-060, Kraków, Poland

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Identyfikatory

DOI

10.2478/s11532-009-0107-y