Mechanistic studies on the effect of veratryl alcohol on the lignin peroxidase catalyzed oxidation of pyrogallol red in reversed micelles

• Autorzy: Jing Lan , Xirong Huang , Ming Hu , Weifeng Liu , Yuezhong Li , Yinbo Qu , Peiji Gao
• Języki publikacji: EN

Abstrakty

EN

The lignin peroxidase (LiP) catalyzed oxidation of pyrogallol red (PR) in the absence and presence of veratryl alcohol (3,4-dimethoxybenzyl alcohol, VA) was carried out in bis (2-ethylhexyl) sulfosuccinate sodium (AOT)/ polyoxyethylene lauryl ether (Brij30) reversed micelles to elucidate the role of VA. Results indicated that VA could accelerate the LiP catalyzed oxidation of PR, especially at low H2O2 concentrations. Unlike in bulk aqueous medium, the protection of LiP by VA in the present medium was relatively unsubstantial, even at high H2O2 concentrations. Analysis of data from a series of experiments showed that the enhancement of the PR oxidation caused by VA was mainly due to the indirect oxidation of PR by VA+∙ from the LiP catalyzed oxidation of VA. It was also found that at the same protector concentration (40 μM), VA (the physiological substrate of LiP) was less effective than PR (a phenolic compound) in protecting LiP from the H2O2 derived inactivation. This novel phenomenon deserves further study. [...]

Słowa kluczowe

EN

Lignin peroxidase; reversed micelle; veratryl alcohol; pyrogallol red; mediated oxidation

• Czasopismo: Open Chemistry
• Rocznik: 2007
• Tom: 5
• Numer: 3
• Strony: 672-687

Daty

wydano

2007-09-01

online

2007-06-09

Twórcy

autor

Jing Lan

• Key Laboratory of Colloid Interface Chemistry of the Education Ministry of China, Shandong University, Jinan, 250100, P.R. China

autor

Xirong Huang,

autor

Ming Hu

• State Key Laboratory of Microbial Technology of China, Shandong University, Jinan, 250100, P.R. China

autor

Weifeng Liu

• State Key Laboratory of Microbial Technology of China, Shandong University, Jinan, 250100, P.R. China

autor

Yuezhong Li

• State Key Laboratory of Microbial Technology of China, Shandong University, Jinan, 250100, P.R. China

autor

Yinbo Qu

• State Key Laboratory of Microbial Technology of China, Shandong University, Jinan, 250100, P.R. China

autor

Peiji Gao

• State Key Laboratory of Microbial Technology of China, Shandong University, Jinan, 250100, P.R. China

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Identyfikatory

DOI

10.2478/s11532-007-0032-x