Oligonucleotides bearing pentaerythritol-derived mass tags

• Autorzy: Anu Kiviniemi , Petri Heinonen , Harri Lönnberg
• Języki publikacji: EN

Abstrakty

EN

Five different di-O-alkylated pentaerythritol phosphoramidite building blocks (2a–e) were synthesized and introduced into oligonucleotides to obtain mass-tagged probes (6a–f) useful in RNA transcription profiling. These non-nucleosidic mass tags allow categorization of oligonucleotide probes having identical nucleoside content and, hence, identification of the probe hybridized to RNA by mass spectrometry analysis. Hybridization properties of the oligonucleotide conjugates were elucidated by melting temperature measurements.

Słowa kluczowe

EN

Oligonucleotide conjugate; pentaerythritol; mass tag; hybridization; phosphoramidite; ether

• Czasopismo: Open Chemistry
• Rocznik: 2007
• Tom: 5
• Numer: 1
• Strony: 191-200

Daty

wydano

2007-03-01

online

2007-03-01

Twórcy

autor

Anu Kiviniemi

• Department of Chemistry, University of Turku, FIN-20014, Turku, Finland

autor

Petri Heinonen

• Department of Chemistry, University of Turku, FIN-20014, Turku, Finland

autor

Harri Lönnberg

• Department of Chemistry, University of Turku, FIN-20014, Turku, Finland,

Bibliografia

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Identyfikatory

DOI

10.2478/s11532-006-0046-9