Synthesis of heptapeptides and analysis of sequence by tandem ion trap mass spectrometry

• Autorzy: Chenxi Jia , Wei Qi , Zhimin He , Haoming Yang , Bin Qiao
• Języki publikacji: EN

Abstrakty

EN

Two heptapeptides have been prepared by Fmoc methodology using Wang resin as solid support. For attachment of the first amino acid, several coupling systems were evaluated, and DIC/DMAP system could give yields of >99% and low levels of racemization. The selection of scavenger combination to deprotect side chains revealed that H2O/p-cresol was good at scavenging trityl and 1,2-ethanedithiol was highly efficient for scavenging t-butyl. Through shortening the preactivation time to 5 min, the racemization which occurred during formation of amide bonds coupled by HBTU was minimized. The crude peptides were characterized by RP-HPLC and MS, and sequenced by MS/MS to acquire reliable amino acid sequence information.

Słowa kluczowe

EN

Peptide synthesis; mass spectrometry; peptide sequencing; racemization; RP-HPLC; scavenger

• Czasopismo: Open Chemistry
• Rocznik: 2006
• Tom: 4
• Numer: 2
• Strony: 285-298

Daty

wydano

2006-06-01

online

2006-06-01

Twórcy

autor

Chenxi Jia

• Chemical Engineering Research Center, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People’s Republic of China

autor

Wei Qi

• Chemical Engineering Research Center, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People’s Republic of China

autor

Zhimin He

• Chemical Engineering Research Center, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People’s Republic of China,

autor

Haoming Yang

• Chemical Engineering Research Center, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People’s Republic of China

autor

Bin Qiao

• Chemical Engineering Research Center, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People’s Republic of China

Bibliografia

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Identyfikatory

DOI

10.2478/s11532-006-0004-6