Label-Free Photoacoustic Cell-Tracking In Real-Time

• Autorzy: Liangzhong Xiang , Moiz Ahmad , Xiang Hu , Zhen Cheng , Lei Xing
• Języki publikacji: EN

Abstrakty

EN

Cell-tracking method has an important role in detection of metastatic circulating tumor cells (CTCs) and cell-based therapies. Label-free imaging techniques are desirable for cell-tracking because they enable long time observations without photobleaching in living cells or tissues where labeling is not always possible. Photoacoustic microscopy is a label-free imaging technique that offers rich contrast based on nonfluorescent cellular optical absorption associated with intrinsic chromophores and pigments. We show here that photoacoustic imaging is feasible for detecting very low numbers (x 104) of melanoma cells without labeling because of the strong instinct optical absorption of melanin in near-infrared wavelength. Flowing melanoma cells are imaged with micrometerresolution (40 μm) and penetration depths of centimeters (13 mm) in real-time. Photoacoustic imaging as a new cell-tracking method provides a novel modality for cancer screening and offers insights into the underlying biological process of cancer growth and metastasis and cell therapy.

• Czasopismo: X-Acoustics: Imaging and Sensing
• Rocznik: 2014
• Tom: 1
• Numer: 1

Daty

otrzymano

2002-03-15

zaakceptowano

2014-04-23

online

2014-09-19

Twórcy

autor

Liangzhong Xiang

• Department of Radiation Oncology, School of Medicine, Stanford University, Stanford, CA USA 94305 USA

autor

Moiz Ahmad

• Department of Radiation Oncology, School of Medicine, Stanford University, Stanford, CA USA 94305 USA

autor

Xiang Hu

• Molecular Imaging Program at Stanford (MIPS), Department of Radiology and Bio-X Program, Canary Center at Stanford for Cancer Early Detection, Stanford University, California, 94305-5344 USA

autor

Zhen Cheng

• Molecular Imaging Program at Stanford (MIPS), Department of Radiology and Bio-X Program, Canary Center at Stanford for Cancer Early Detection, Stanford University, California, 94305-5344 USA

autor

Lei Xing

• Department of Radiation Oncology, Stanford University, 875 Blake Wilbur Drive Room G233, Stanford, CA 94305-5847, Ph: [phone], Fax: [phone],

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Identyfikatory

DOI

10.2478/phto-2014-0002