rapid detection for the inhibition of phosphoglucose isomerase from Escherichia coli by mercury(II) chloride based on TLC-autographic analysis – preliminary studies

• Autorzy: Katarzyna Paradowska , Joanna Lutek , Grazyna Ginalska
• Języki publikacji: EN

Abstrakty

EN

The quest for new techniques for screening inhibitors of phosphoglucose isomerase is crucially important owing to therapeutic control of chronic bacterial infections associated with the biosynthesis of bacterial biofilm. According to the new method, yellowish zones against the purple background could be visually observed where phosphoglucose isomerase activity was inhibited. The new protocol with NADPH/NBT/PMS staining for TLC-autographic method was able to detect PGI inhibition by pure reference substance as mercury(II) chloride.

Słowa kluczowe

EN

TLC-autography; phosphoglucose isomerase; inhibitor; E. coli ATCC 25922; biofilm

• Czasopismo: Current Issues in Pharmacy and Medical Sciences
• Rocznik: 2014
• Tom: 27
• Numer: 2
• Strony: 127-130

Daty

wydano

2014-06-01

otrzymano

2014-02-21

zaakceptowano

2014-08-11

online

2014-11-25

Twórcy

autor

Katarzyna Paradowska

• Chair and Department of Biochemistry and Biotechnology, Medical University of Lublin, Poland,

autor

Joanna Lutek

• Student of Chair and Department of Biochemistry and Biotechnology, Medical University of Lublin, Poland

autor

Grazyna Ginalska

• Chair and Department of Biochemistry and Biotechnology, Medical University of Lublin, Poland

Bibliografia

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Identyfikatory

DOI

10.2478/cipms-2014-0030