Determination of caffeic acid in root and rhizome of Black cohosh (Cimicifuga racemosa (L.) Nutt.)

• Autorzy: Karolina Zapala , Grazyna Szymczak , Magdalena Wojciak-Kosior
• Języki publikacji: EN

Abstrakty

EN

Cimicifuga racemosa, is a plant with a diverse and long history of medicinal use. Caffeic acid, bioactive compound, which often occurs with other polyphenols can influence the biological activity of this plant. The aim of our work was quantitative analysis of caffeic acid in roots and rhizomes of two varieties of C. racemosa. Analysis was performed by HPLC method. The extracts were separated on C18 reversed-phase column using mixture of methanol, water and formic acid (25:75:0.5 v/v/v) as a mobile phase. The flow rate of eluent was 1.0 ml·min-1. The obtained validation parameters such as linearity, linear regression equation and precision expressed as a relative standard deviation were adequate for quantitative determination. Caffeic acid was found in all tested extracts. The highest total amount of caffeic acid was determined in C. racemosa var. racemosa (255.3 μg·g-1) while its concentration in C. racemosa var. cordifolia was significantly lower (213.0 μg·g-1).

Słowa kluczowe

EN

caffeic acid; Cimicifuga racemosa; phenolic acids

• Czasopismo: Current Issues in Pharmacy and Medical Sciences
• Rocznik: 2014
• Tom: 27
• Numer: 2
• Strony: 108-110

Daty

wydano

2014-06-01

otrzymano

2014-07-07

zaakceptowano

2014-07-17

online

2014-11-25

Twórcy

autor

Karolina Zapala

• Department of Analytical Chemistry, Medical University of Lublin, Chodzki 4a, 20-093, Lublin, Poland

autor

Grazyna Szymczak

• Botanical Garden of Maria Curie-Skłodowska University in Lublin, Slawinkowska 3, 20-810 Lublin, Poland

autor

Magdalena Wojciak-Kosior

• Department of Analytical Chemistry, Medical University of Lublin, Chodzki 4a, 20-093, Lublin, Poland,

Bibliografia

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Identyfikatory

DOI

10.2478/cipms-2014-0025