Czasopismo
Tytuł artykułu
Warianty tytułu
Języki publikacji
Abstrakty
Cooperative binding is commonly observed in biological receptor systems. This study investigates whether it is possible to prepare nano-sized molecularly imprinted polymers (nanoMIPs) that show cooperative binding. NanoMIPs which exhibit cooperative binding would have increased affinity for immobilised template molecules making them useful for advanced applications in diagnostics and sensors. The use of a templatederivatised solid support provides a facile route to prepare nanoMIPs with surface imprints, and the method is ideally suited to study this topic. Although not observed during the course of this study, positive interbinding site cooperativity was hypothesised by way of an increase in the number of binding sites imprinted on the nanoMIPs, by increasing template density on the solid support surface. After synthesis, the affinity of nanoMIPs was analysed using surface plasmon resonance (SPR) technique. Under the conditions investigated, a ten fold increase in binding affinity was measured as template density was increased. SPR results could be explained by an increase in cooperative binding; however calculations showed that the increase in affinity was not significant enough to prove cooperative binding interactions. The main conclusion obtained was that MIP nanoparticles contain only one “high-affinity” binding site that interacts with immobilised template in an SPR assay.
Czasopismo
Rocznik
Tom
Numer
Opis fizyczny
Daty
otrzymano
2015-06-30
zaakceptowano
2015-09-09
online
2016-02-08
Twórcy
autor
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Department of Chemistry, College of Science and Engineering,
University of Leicester, LE1 7RH, UK
autor
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Department of Chemistry, College of Science and Engineering,
University of Leicester, LE1 7RH, UK
autor
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Department of Chemistry, College of Science and Engineering,
University of Leicester, LE1 7RH, UK
autor
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Department of Chemistry, College of Science and Engineering,
University of Leicester, LE1 7RH, UK
autor
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Department of Chemistry, College of Science and Engineering,
University of Leicester, LE1 7RH, UK
autor
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Department of Chemistry, College of Science and Engineering,
University of Leicester, LE1 7RH, UK
Bibliografia
- [1] J. A. Goodrich and J. F. Kugel, in Binding and Kinetics forMolecular Biologists, Cold Spring Harbor Laboratory Press,New York, 2007, 49–68.
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- [4] M. Ptashne and A. Gann, Genes & Signals, Cold Spring HarborLaboratory Press, New York, 2002.
- [5] S. A. Piletsky, N. W. Turner and P. Laitenberger, Molecularlyimprinted polymers in clinical diagnostics--future potential andexisting problems, Med. Eng. Phys., 2006, 28, 971–7.[Crossref]
- [6] H. S. Andersson, J. G. Karlsson, S. a. Piletsky, A. C.Koch-Schmidt, K. Mosbach and I. a. Nicholls, Study of thenature of recognition in molecularly imprinted polymers, II
- [1]: Influence of monomer-template ratio and sample load onretention and selectivity, J. Chromatogr. A, 1999, 848, 39–49.
- [7] J. Svenson, J. G. Karlsson and I. A. Nicholls, Nuclear magneticresonance study of the molecular imprinting of (−)-nicotine:template self-association, a molecular basis for cooperativeligand binding, J. Chromatogr. A, 2004, 1024, 39–44.
- [8] P. Sajonz, M. Kele, G. Zhong, B. Sellergren and G. Guiochon,Study of the Thermodynamics and Mass Transfer Kinetics ofTwo Enantiomers on a Polymeric Imprinted Staionary Phase, J.Chromatogr. A, 1998, 810, 1–17.
- [9] K. Haupt, Imprinted polymers: The next generation, Anal.Chem., 2003, 75, 376–383.
- [10] Editorial, Capturing cooperativity, Nat. Chem. Biol., 2008, 4,433.
- [11] J. A. Goodrich and J. F. Kugel, in Binding and Kinetics forMolecular Biologists, Cold Spring Harbor Laboratory Press,New York, 2007, pp. 1 – 18.
- [12] A. R. Guerreiro, I. Chianella, E. Piletska, M. J. Whitcombe andS. A. Piletsky, Selection of imprinted nanoparticles by affinitychromatography, Biosens. Bioelectron., 2009, 24, 2740–2743.[Crossref][WoS]
- [13] S. A. Piletsky, K. Karim, E. V. Piletska, a. P. F. Turner, C. J.Day, K. W. Freebairn and C. Legge, Recognition of ephedrineenantiomers by molecularly imprinted polymers designedusing a computational approach, Analyst, 2001, 126,1826–1830.
- [14] E. Moczko, A. Poma, A. Guerreiro, I. Perez de VargasSansalvador, S. Caygill, F. Canfarotta, M. J. Whitcombe and S.Piletsky, Surface-modified multifunctional MIP nanoparticles,Nanoscale, 2013, 5, 3733–41.
- [15] Y. Hoshino, T. Kodama, Y. Okahata and K. J. Shea, Peptideimprinted polymer nanoparticles: A plastic antibody, J. Am.Chem. Soc., 2008, 130, 15242–15243.
- [16] F. Breton, R. Rouillon, E. V. Piletska, K. Karim, A. Guerreiro, I.Chianella, S. A. Piletsky, Virtual imprinting as a tool to designefficient MIPs for photosynthesis-inhibiting herbicides,Biosens. Bioelectron., 2006, 22, 1948-1954.[WoS][Crossref]
- [17] G. Vasapollo, R. Del Sole, L. Mergola, M. R. Lazzoi, A. Scardino,S. Scorrano and G. Mele, Molecularly imprinted polymers:present and future prospective, Int. J. Mol. Sci., 2011, 12,5908–45.[Crossref]
- [18] J. Svenson , H. Andersson, S. A. Piletsky, I. A. Nicholls,Spectroscopic studies of the molecular imprintingself-assembly process, J. Mol. Recognit., 1998, 11, 83-86.[Crossref]
- [19] E. V. Piletska, L. D. Stavroulakis, M. J. Whitcombe , A. Sharma,S. Primrose, G. K. Robinson, S. A. Piletsky, Passive controlof quorum sensing: prevention of pseudomonas aeruginosabiofilm formation by imprinted polymers, Biomacromolecules,2011, 12, 1066-1071.[Crossref][WoS]
- [20] J. P. Rosengren-Holmberg, J. G. Karlsson, J. Svenson, H. S.Andersson and I. A. Nicholls, Molecularly imprinted polymers:present and future prospective, Org. Biomol. Chem., 2009, 7,3148.
- [21] A. Poma, A. Guerreiro, M. J. Whitcombe, E. V. Piletska, A. P. F.Turner and S. a. Piletsky, Solid-Phase Synthesis of MolecularlyImprinted Polymer Nanoparticles with a Reusable Template-“Plastic Antibodies”, Adv. Funct. Mater., 2013, 23, 2821–2827.[Crossref][WoS]
- [22] S. Subrahmanyam, A. Guerreiro, A. Poma, E. Moczko, E.Piletska and S. Piletsky, Optimisation of experimentalconditions for synthesis of high affinity MIP nanoparticles, Eur.Polym. J., 2013, 49, 100–105. [WoS][Crossref]
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.-psjd-doi-10_1515_molim-2015-0003