Effects of hypothermia on skeletal ischemia reperfusion injury in rats

• Autorzy: Şafak Ekinci , Ümit Kaldırım , Faruk Akyıldız , Serkan Bilgiç , Kenan Koca , Yavuz Poyrazoğlu , Ozgür Selim Uysal , Hasan Turğut , Selim Türkkan , Ömer Erşen , Turgut Topal , Huseyin Ozkan
• Języki publikacji: EN

Abstrakty

EN

Objective: The aim of this study was to investigate the effect of hypothermia (H) on skeletal ischemia-reperfusion (IR) injury in rats by measuring malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), nitric oxide (NO), and interleukin-1 beta (IL-1β) in muscle, and measureing immunohistochemical- inducible nitric oxide synthase (iNOS) staining of skeletal muscle. Materials and Methods: Eighteen Wistar Albino rats were divided randomly into three groups (sham, IR, hypothermia) (n=6). The sham group had all procedures without the IR period. The lower right extremity of rats in the IR and hypothermia groups was subjected to 2 hours of ischemia and 22 hours of reperfusion by applying a clamp on the common iliac artery and a rubber-band at the level of the lesser trochanter under general anesthesia. Rats in the hypothermia group underwent 4 hours of hypothermia during the first four hours of reperfusion in addition to a 2-hour ischemia and 22-hour reperfusion period. All rats were sacrificed at end of the IR period using a high dose of anesthesia. The tibialis anterior muscles were preserved. Immunohistochemical iNOS staining was performed, and MDA, SOD, GSH-Px, NO, and IL-1β were measured in the muscle. Results: The level of MDA, NO, and IL-1β in muscle was increased in the IR group compared with that in the sham group, but these parameters were decreased in the hypothermia group compared with the IR group. The activities of SOD and GSH-Px in muscle were decreased in the IR group; however, these parameters were increased in the hypothermia group. The score and intensity of iNOS staining of skeletal muscle was dens in IR group, mild in hypothermia group, and weak in sham group. Conclusion: The present study has shown that hypothermia reduced IR injury in the skeletal muscle by decreasing the levels of MDA, NO, and IL-1β, and increasing the activities of SOD and GSH-Px. In addition, hypothermia attenuated the score and intensity of iNOS staining.

Słowa kluczowe

EN

IR injury; hypothermia; MDA; NO; IL-1β; SOD; GSH-Px; iNOS staining

• Czasopismo: Open Medicine
• Rocznik: 2014
• Tom: 10
• Numer: 1

Daty

otrzymano

2014-04-14

zaakceptowano

2015-01-12

online

2015-02-27

Twórcy

autor

Şafak Ekinci

• Department of Orthopaedics and Traumatology, Malatya Military Hospital, Malatya, Turkey

autor

Ümit Kaldırım

• Department of Emergency Medicine, Gulhane Military Medical Academy, Ankara, Turkey

autor

Faruk Akyıldız

• Department of Orthopaedics and Traumatology, Malatya Military Hospital, Malatya, Turkey

autor

Serkan Bilgiç

• Department of Orthopaedics and Traumatology, Haydarpaşa Military Hospital, İstanbul, Turkey

autor

Kenan Koca

• Department of Orthopaedics and Traumatology, Gulhane Military Medical Academy, Ankara, Turkey

autor

Yavuz Poyrazoğlu

• Department of General Surgery, Mevki Military Hospital, Ankara, Turkey

autor

Ozgür Selim Uysal

• Department of Emergency Medicine, Gulhane Military Medical Academy, Ankara, Turkey

autor

Hasan Turğut

• Department of Orthopaedics and Traumatology, Bursa Military Hospital, Bursa, Turkey

autor

Selim Türkkan

• Department of Orthopaedics and Traumatology, Gulhane Military Medical Academy, Ankara, Turkey

autor

Ömer Erşen

• Department of Orthopaedics and Traumatology, Erzurum Military Hospital, Erzurum, Turkey

autor

Turgut Topal

• Department of Physiology, Gulhane Military Medical Academy, Ankara, Turkey

autor

Huseyin Ozkan

• Department of Orthopaedics and Traumatology, Gulhane Military Medical Academy, Ankara, Turkey

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Identyfikatory

DOI

10.1515/med-2015-0031