Survival of Bifidobacteria and their usefulness in Faecal Source Tracking

• Autorzy: Catherine D. Luyt , S. M. M. Khamanga , W. J. Muller , Roman Tandlich
• Języki publikacji: EN

Abstrakty

EN

Bifidobacteria have long since been recommended as indicators of human and animal pollution. Concentration ratio (tracking ratio) of the sorbitol-utilising bifidobacteria (SUB) and the total bifidobacteria (TB) can be used to distinguish between animal and human sources of faecal water contamination. The cut-off value needs to be calibrated in a given geographical area. Seven sites with permanent faecal contamination were selected in South Africa. Concentrations of SUB ranged from 10-50000 cells/100 mL, while TB ranged from 0-8000 cells/100 mL. The tracking ratio ranged from 0.10 to 6.25, but no clear cut-off value could be established. The YN-17 agar was replaced for TB with the modified Beerens medium with pH = 5.70, to suppress the growth of faecal streptococci. Tracking ratios observed are most likely the results of different survival rates of SUB and TB. Bifidobacteria die-off due to nutrients was not found to be significant using design of experiment. Thus a lack of continuous input or oxygen levels in water may be major factors. This would limit the ratios used as a faecal source tracking method.

Słowa kluczowe

EN

bifidobacteria; faecal source tracking; waterquality

• Czasopismo: Liquid Waste Recovery
• Rocznik: 2015
• Tom: 1
• Numer: 1

Daty

online

2015-07-16

Twórcy

autor

Catherine D. Luyt

• Environmental Health and Biotechnology
  Research Group, Division of Pharmaceutical Chemistry, Faculty of
  Pharmacy, Rhodes University, South Africa

autor

S. M. M. Khamanga

• Division of Pharmaceutics, Faculty of Pharmacy,
  Rhodes University, South Africa

autor

W. J. Muller

• Amatole Waterboard, East London, South Africa

autor

Roman Tandlich

• Environmental Health and Biotechnology
  Research Group, Division of Pharmaceutical Chemistry, Faculty of
  Pharmacy, Rhodes University, South Africa

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Identyfikatory

DOI

10.1515/lwr-2015-0001