Analysis of oligonucleotides by liquid chromatography with alkylamide stationary phase

• Autorzy: Bogusław Buszewski , Zahra Safaei , Sylwia Studzińska
• Języki publikacji: EN

Abstrakty

EN

The main aim of the present investigation was to determine retention behavior and interactions of oligonucleotides with alkylamide stationary phase. Five oligonucleotides, differing in the sequence, were tested. Changes in the composition of the mobile phase, i.e. pH (5.0−7.0) and buffer concentration (30−75 mM) were investigated in detail. In addition, the hydrophobic and electrostatic parameters were measured for the different pH’s and salt concentrations. The theoretical model of interactions between individual elements of the separation system, (e.g. solute, stationary phase, and mobile phase) based on zeta potential measurements, hydrophobic and electrostatic parameters calculation, and molecular modeling, has been considered.

EN

Słowa kluczowe

EN

oligonucleotide; mobile phase; alkylamide stationary phase; interactions; zeta potential

• Czasopismo: Open Chemistry
• Rocznik: 2015
• Tom: 13
• Numer: 1

Daty

otrzymano

2015-01-16

zaakceptowano

2015-09-14

online

2015-11-25

Twórcy

autor

Bogusław Buszewski

• Chair of the Environmental Chemistry & Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, 7 Gagarin St. PL 87-100 Torun, Poland

autor

Zahra Safaei

• Chair of the Environmental Chemistry & Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, 7 Gagarin St. PL 87-100 Torun, Poland

autor

Sylwia Studzińska

• Chair of the Environmental Chemistry & Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, 7 Gagarin St. PL 87-100 Torun, Poland

Bibliografia

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Identyfikatory

DOI

10.1515/chem-2015-0141