Influence of extraction methods on the recovery of astragaloside IV from the roots ofAstragalus mongholicusin Soxhlet- and Twisselmann-type apparatus

• Autorzy: Wirginia Kukuła-Koch , Tomasz Baj , Elwira Sieniawska , Wojciech Koch , Jarosław Widelski , Katarzyna Luchowska , Barbara Kołodziej , Kazimierz Głowniak
• Języki publikacji: EN

Abstrakty

EN

Astragalus mongholicus, commonly used in Traditional Chinese Medicine (TCM), contains significant amounts of triterpene saponins – astragalosides, which possess a wide variety of pharmacological actions, including the treatment of cardiovascular and respiratory diseases. The present study was performed to develop a fast and effective extraction method of saponins from crude plant material using Twisselmann-type apparatus. Moreover, a detailed comparison between Soxhlet and Twisselmann apparatus was performed to explain the advantages and disadvantages of their applications. The content of astragaloside IV – the major saponin of A. mongholicus extracts and chosen as an extraction efficiency marker ‒ was determined by means of RP-HPLCELSD chromatography in an herein optimized method. Twisselmann extraction shortened its duration from 4 to 2 hours in comparison with the Soxhlet method ‒ a generally accepted method preparation for this extract. The effectiveness of both apparatus was alike (3.24 ± 0.44 mg and 3.48 ± 0.26 mg of astragaloside IV/ 4g of powdered root for 2 hours in Twisselmann and 4 hours in Soxhlet apparatus). Compared with a pharmacopoeial method, the application of Twisselmann apparatus enabled a decrease in analysis time of 50%, which is of high significance, especially for large, scaled-up industrial scale.

EN

Słowa kluczowe

EN

Astragalus mongholicus; Twisselmann extraction; HPLC-ELSD detection; saponins; astragaloside IV

• Czasopismo: Open Chemistry
• Rocznik: 2015
• Tom: 13
• Numer: 1

Daty

otrzymano

2014-12-03

zaakceptowano

2015-04-01

online

2015-06-02

Twórcy

autor

Wirginia Kukuła-Koch

• Chair and
  Department of Pharmacognosy with Medical Plant Unit, Medical
  University of Lublin, 1, Chodźki Str., 20-093 Lublin, Poland

autor

Tomasz Baj

• Chair and
  Department of Pharmacognosy with Medical Plant Unit, Medical
  University of Lublin, 1, Chodźki Str., 20-093 Lublin, Poland

autor

Elwira Sieniawska

• Chair and
  Department of Pharmacognosy with Medical Plant Unit, Medical
  University of Lublin, 1, Chodźki Str., 20-093 Lublin, Poland

autor

Wojciech Koch

• Chair and Department of Food and Nutrition, Medical
  University of Lublin, 4a, Chodźki Str., 20-093 Lublin, Poland

autor

Jarosław Widelski

• Chair and
  Department of Pharmacognosy with Medical Plant Unit, Medical
  University of Lublin, 1, Chodźki Str., 20-093 Lublin, Poland

autor

Katarzyna Luchowska

• Department of Industrial
  and Medicinal Plants, University of Life Sciences, 15, Akademicka Str.,
  20-950 Lublin, Poland

autor

Barbara Kołodziej

• Department of Industrial
  and Medicinal Plants, University of Life Sciences, 15, Akademicka Str.,
  20-950 Lublin, Poland

autor

Kazimierz Głowniak

• Chair and
  Department of Pharmacognosy with Medical Plant Unit, Medical
  University of Lublin, 1, Chodźki Str., 20-093 Lublin, Poland

Bibliografia

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Identyfikatory

DOI

10.1515/chem-2015-0108