Application of QuEChERS - High Performance Liquid Chromatography with Postcolumn Fluorescence Derivatization (HPLC-FLD) method to analyze Eprinomectin B1a residues from apour-onconditioning in bovine edible tissues

• Autorzy: Ana Csuma , Romeo T. Cristina , Eugenia Dumitrescu , Florin Muselin , Ersilia C. Alexa , Monica Butnariu , Iosif Gergen
• Języki publikacji: EN

Abstrakty

EN

A QuEChERS in house method for determining the marker residue of eprinomectin (eprinomectin B1a) by HPLC-FLD in bovine tissues and milk provided from treated animals was developed and applied. Briefly: all samples were extracted with acetonitrile using a dispersive SPE purification stage. The ascertained detection limits were 1 μg kg-1 and the quantification limits 2 μg kg-1. Recoveries on tissue samples fortified in the range of 10 μg kg-1 to 200 μg kg-1 were from 80.0% to 87.2%, with variation coefficients between 2.7% to 10.6%. The confirmation of residues in the purified extracts was made by LC-MS/MS after separation on an XTerra MS C18 (10 cm × 2.1 mm, 3.5 μm) column with a mobile phase of acetonitrile / formic acid 0.1% (97:3, v/v) at a flow rate of 0.2 mL min-1 and MRM monitoring of three characteristic ions (m/z 896.1, m/z 467.9 and m/z 329.9), resulting from the fragmentation of molecular ions [M-H]+ (m/z 914.6) of eprinomectin and the comparison of the abundance ratio of fragmented ions was obtained in the booth, sample and standard at comparative concentrations. In conclusion, this method has proven its advantage and versatility as a routine drug residues analysis method.

EN

Słowa kluczowe

EN

Eprinomectin B1a; QuEChERS HPLC-FLD; Bovine edible tissues; Residues

• Czasopismo: Open Chemistry
• Rocznik: 2015
• Tom: 13
• Numer: 1

Daty

otrzymano

2014-10-29

zaakceptowano

2015-01-16

online

2015-02-12

Twórcy

autor

Ana Csuma

• N.S. Pasteur Institute, Bucharest Romania

autor

Romeo T. Cristina

• Banat’s University
  of Agriculture and Veterinary Medicine “King Michael I of Romania”
  from Timisoara (BUAMVT), 119, Calea Aradului, 300645, Timisoara,
  Romania. Department of Pharmacology and Pharmacy

autor

Eugenia Dumitrescu

• Banat’s University
  of Agriculture and Veterinary Medicine “King Michael I of Romania”
  from Timisoara (BUAMVT), 119, Calea Aradului, 300645, Timisoara,
  Romania. Department of Pharmacology and Pharmacy

autor

Florin Muselin

• BUAMVT, Romania, Faculty of Veterinary Medicine,
  Department of Toxicology

autor

Ersilia C. Alexa

• BUAMVT, Romania, Faculty of Food
  Technology, Food control and expertise Dept.

autor

Monica Butnariu

• BUAMVT, Romania, Chemistry and Biochemistry
  Depts.

autor

Iosif Gergen

• BUAMVT, Romania, Faculty of Food
  Technology, Food control and expertise Dept.

Bibliografia

• [1] Inchem WHO Food Additives Series 41: eprinomectin.,The 50th meeting of the Joint FAO/WHO Expert Committee onFood Additives (JECFA) Geneva, 1998, http://www.inchem.org/documents/jecfa/jecmono/v041je02.htm
• [2] Wells R.G., Eprinomectin, In: Residues of Some Veterinary Drugsin Animals and Food: Monographs prepared by the FiftiethMeeting Joint FAO/WHO Expert Committee on Food Additives(JECFA) Meeting, Rome, 17-26 February 1998, 41, 31, 1999,http://books.google.ro/books?id=jMCHWPfDYf4C&printsec=frontcover&hl=ro&source=gbs_ge_summary_r&cad=0#v=onepage&q&f=false
• [3] Macrocyclic Lactones (Veterinary - Systemic), The United StatesPharmacopeia Convention Inc., 2006, http://www.windhound.com/eng/macrocyclicLactones.pdf
• [4] EMEA/MRL/114/96 Final, Eprinomectin – Summary Report,http://www.ema.europa.eu/docs/en_GB/document_library/Maximum_Residue_Limits_-_Report/2009/11/WC500014177.pdf
• [5] Commission Regulation (EU) No 37/2010 on pharmacologicallyactive substances and their classification regarding maximumresidue limits in foodstuffs of animal origin, http://ec.europa.eu/health/files/mrl/mrl_20101212_consol.pdf
• [6] Shoop W., Soll M., Ivermectin, abamectin and eprinomectin,In: J. Vercruysse and R.S. Rew, (Eds.), Macrocyclic lactones inantiparasitic therapy, CABI Publishing, Oxon, U.K., 2002.
• [7] Shoop W.L., DeMontigny P., Fink D.W., Williams J.B., Egerton J.R.,Mrozik H., et al., Int. J. Parasitol., 1996, 26, 1227-1235.[Crossref]
• [8] Sheridan R., Desjardins L., J. AOAC Int., 2006, 89, 1088-1094.
• [9] Riviere J.E., Papich M.G., Veterinary Pharmacology andTherapeutics, Ninth Edition, Wiley & Blackwell, Ames, Iowa,USA, 2013.
• [10] Floate K.D., Can. J. Vet. Res., 2006, 70, 1-10.
• [11] Merck & Co., Ivomec® Eprinex™ (eprinomectin) Pour-On for Beefand Dairy Cattle, Environmental Assessment, 1996, http://www.fda.gov/downloads/AnimalVeterinary/DevelopmentApprovalProcess/EnvironmentalAssessments/UCM072375.pdf
• [12] McKellar Q.A., Gokbulut C., Curr. Pharm. Biotechnol., 2012, 13,888-991.[Crossref]
• [13] Kiki-Mvouaka S., Menez C., Borin C., Lyazrhi F., Foucaud-Vignault M., Dupuy J., Drug. Metab. Dispos., 2010, 38, 573-580.[Crossref]
• [14] Alvinerie M., Sutra J.F., Galtier P., Mage C., Res. Vet. Sci., 1999,67, 229-232.
• [15] Baoliang P., Yuwan W., Zhende P., Lifschitz A.L., Ming W., Vet.Res. Commun., 2006, 30, 263-270.
• [16] Bassissi M.F., Alvinerie M., Lespine A., Comp. Biochem. Physiol.C Toxicol. Pharmacol., 2004, 138, 437-444.
• [17] Bengone-Ndong T., Ba M.A., Kane Y., Sané I., Sutra J.F., AlvinerieM., Parasitol. Res., 2006, 98, 501-506.
• [18] Wen H., Pan B., Wang Y., Wang F., Yang Z., Wang M., Vet.Parasitol., 2010, 174, 72-76.
• [19] Sutra J.F., Chartier C., Galtier P., Alvinerie M., Analyst, 1998, 123,1525-1527.
• [20] Danaher M., O’Keeffe M., Glennon J., Howells L., Analyst, 2001,126, 576-580.
• [21] Ali M.S., Sun T., McLeroy G.E., Phillippo E.T., J AOAC Int., 2000,83, 31-38.
• [22] Ali M.S., Sun T., McLeroy G.E., Phillippo E.T., J. AOAC Int., 2000,83, 39-52.
• [23] Rudik I., Cummings M.R., Poppenga R.H., J. Vet. Diagn. Invest.,2002, 14, 295-302.[Crossref]
• [24] Kinsella B., Lehotay S.J., Mastovska K., Lightfield A.R., Furey A.,Danaher M., Chim. Acta, 2009, 637, 196-207.
• [25] Directive 2004/9/EC of the European parliament and of theCouncil of 11 February 2004 on the inspection and verification ofgood laboratory practice (GLP), http://ec.europa.eu/enterprise/sectors/chemicals/documents/specific-chemicals/laboratorypractice/index_en.htm
• [26] Directive 2004/10/EC of the European parliament and of theCouncil of 11 February 2004 on the harmonisation of laws,regulations and administrative provisions relating to theapplication of the principles of good laboratory practice and theverification of their applications for tests on chemical substances,http://ec.europa.eu/enterprise/sectors/chemicals/documents/specific-chemicals/laboratory-practice/index_en.htm
• [27] Commission Decision 2002/657/EC implementing CouncilDirective 96/23/EC concerning the performance of analyticalmethods and the interpretation of results.
• [28] Jiang H., Ding S., Xu F., Zhao S., He J., Liu J., et al., Chromatographia,2007, 66, 411-414.
• [29] Miller J.C., Miller J.N., Statistics for analytical chemistry, SixthEdition, Gosport, U.K., Ashford Colour Press Ltd., 2010.
• [30] Durden D.A., J. Chromatogr. B, 2007, 850, 134-146.
• [31] Ordin nr. 187 din 31 octombrie 2007, pentru aprobarea Normeisanitare veterinare privind Codul produselor medicinaleveterinare (In Romanian), 2007, http://www.ansvsa.ro/documente/Ordin_187_2007_Codul_produselor_medicinale_veterinare_262ro.pdf

Identyfikatory

DOI

10.1515/chem-2015-0097