Sterilization of bacteria suspensions and identification of radicals deposited during plasma treatment

• Autorzy: Nevena Puač , Maja Miletić , Miloš Mojović , Ana Popović-Bijelić , Dragana Vuković , Biljana Miličić , Dejan Maletić , Saša Lazović , Gordana Malović , Zoran Lj. Petrović
• Języki publikacji: EN

Abstrakty

EN

In this paper we will present results for plasma sterilization of planktonic samples of two reference strains of bacteria, Pseudomonas aeruginosa ATCC 27853 and Enterococcus faecalis ATCC 29212. We have used a plasma needle as a source of non-equilibrium atmospheric plasma in all treatments. This device is already well characterized by OES, derivative probes and mass spectrometry. It was shown that power delivered to the plasma is bellow 2 W and that it produces the main radical oxygen and nitrogen species believed to be responsible for the sterilization process. Here we will only present results obtained by electron paramagnetic resonance which was used to detect the OH, H and NO species. Treatment time and power delivered to the plasma were found to have the strongest influence on sterilization. In all cases we have observed a reduction of several orders of magnitude in the concentration of bacteria and for the longest treatment time complete eradication. A more efficient sterilization was achieved in the case of gram negative bacteria.

Słowa kluczowe

EN

plasma needle; sterilization; spin-trap; Electron Paramagnetic Resonance; radicals

• Czasopismo: Open Chemistry
• Rocznik: 2015
• Tom: 13
• Numer: 1

Daty

otrzymano

2014-01-31

zaakceptowano

2014-05-29

online

2014-11-26

Twórcy

autor

Nevena Puač

• Institute of Physics, University of Belgrade, 11080 Belgrade, Serbia

autor

Maja Miletić

• Faculty of Dental Medicine, University of Belgrade, 11000 Belgrade, Serbia

autor

Miloš Mojović

• Faculty of Physical Chemistry, University of Belgrade, University of Belgrade, 11000 Belgrade, Serbia

autor

Ana Popović-Bijelić

• Faculty of Physical Chemistry, University of Belgrade, University of Belgrade, 11000 Belgrade, Serbia

autor

Dragana Vuković

• Institute of Microbiology and Immunology, Faculty of Medicine, University of Belgrade, 11000 Belgrade, Serbia

autor

Biljana Miličić

• Faculty of Dental Medicine, University of Belgrade, 11000 Belgrade, Serbia

autor

Dejan Maletić

• Institute of Physics, University of Belgrade, 11080 Belgrade, Serbia

autor

Saša Lazović

• Institute of Physics, University of Belgrade, 11080 Belgrade, Serbia

autor

Gordana Malović

• Institute of Physics, University of Belgrade, 11080 Belgrade, Serbia

autor

Zoran Lj. Petrović

• Institute of Physics, University of Belgrade, 11080 Belgrade, Serbia

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Identyfikatory

DOI

10.1515/chem-2015-0041