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1

Zastosowanie materiałów mezoporowatych jako adsorbentów substancji o właściwościach przeciwutleniających

Moritz Michał, Stoińska Aleksandra, Geszke-Moritz Małgorzata

Przemysł Chemiczny

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2024

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T. 103, nr 9

990--993

PL

Przegląd literaturowy dotyczący zastosowania materiałów mezoporowatych MCM-41 i MCM-48, także modyfikowanych organicznymi grupa mi funkcyjnymi, jako adsorbentów wybranych substancji o właściwościach przeciwutleniających. Dyskutowano zastosowanie adsorbentów jako nośników służących uwalnianiu wybranych kwasów fenolowych lub flawonoidów. Przedstawiono także adsorbenty o strukturze organicznej, dla substancji o charakterze flawonoidów.

EN

A review, with 22 refs., of the use of MCM-41 and MCM-48 mesoporous materials, also modified with org. functional groups, as adsorbents of selected substances with antioxidant properties. Adsorbents with org. structure applied to flavonoid compds. were also presented. The use of adsorbents as carriers for the release of selected phenolic acids or flavonoids was discussed.

2

Organic Fertilizer Alleviates Salt Stress in Shallot by Modulating Plant Physiological Responses

Anwar Nur Hasanah, Karyawati Anna Satyana, Maghfoer Moch Dawam, Kurniawan Andi

Journal of Ecological Engineering

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2024

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Vol. 25, nr 7

286--294

EN

Salinity is a major constraint for crop productivity as it reduces agricultural land area. This problem can be ameliorated by the application of organic materials such as manure, which plays an important role in supporting plant growth and reducing soil toxicity by binding toxic compounds. The purpose of this study is to analyse the effect of manure in overcoming the impact of salt stress on shallots. Here randomised block design (RBD) consisting of 2 factors and 3 replications was used. The first factor is salinity levels (0, 50, 100, and 150 mM), and the second is manure doses (0, 10 and 20 t·ha-1). This study finds that the application of 20 t·ha-1 of manure decreases the shallot’s leaf tissue thickness, but the 50 mM of salinity significantly increases it. Then, the application of 20 t·ha-1 of manure increases the shallot’s number of tillers and bulbs, while the 100 mM of salinity significantly decreases its number of tillers. The application of 10 t·ha-1 of manure decreases the proline and flavonoids content of the plant’s leaves. In addition, plants treated and not treated with manure under 50 mM of salinity have higher proline and f lavonoids levels in their leaf. Therefore, shallots can grow under salinity conditions if manure is applied.

3

The Sulfur Dioxide Absorption Capacity of Plants Tecoma Stans and its Effect on Antioxidants

Al-Jaba Sura Razzaq Manhee, Al-Janabi Ameera O. Hussain

Ecological Engineering & Environmental Technology

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2024

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Vol. 25, iss. 9

268--276

EN

The current study used to prove the efficiency of Tecoma stans to sulfur dioxide gas (SO2 )was at a concentration of 10 mg·m-3 which is equal to 3.82 ppm for the period from summer exposure (May – June 2023) to reduce SO2 thus reduce some gaseous pollutants that cause global warming and some air pollutants and know the effect of seasonal conditions to mitigate these pollutants. The physiological changes of the three replicates of study plant were observed through equal time periods daily for a period of seven days covered with polyethylene under controlled conditions represented by a greenhouse. The process was repeated three times between the three exposures provide rest periods for the plant for a week. During summer exposure, it was found that the concentration of flavonoids was significantly increased as compare to control (5.222 ± 0.27 mg/100 ml of extract) from 6.58 ± 0.43 to 6.24 ± 0.31 mg/100 ml of extract, but this concentration was increased after the third exposure into to 6.99 ± 0.29 mg/100 ml of extract. There was decrease in Tannins concentration after the second exposure (with concentration 1.5 ± 0.05 to 0.72 ± 0.01 µl/ml)), but this concentration was returned increased significantly to 1.36 ± 0.01 µl/ml) after the third exposure to SO2 . Also in The enzyme activities for peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT) demonstrated varied responses to SO2 exposure, and T. stans showing distinct patterns of enzyme activation. The effect of POD and CAT increased in plants which exposure into SO2 , whereas CAT play important role in inhibition of ROS.

4

Phytochemicals, Antioxidant Activity, and Antimicrobial Effects of the Fractions of Corrigiola telephiifolia Pourr.

El Yaagoubi Wissal, Soussi Meriem, Aazza Smail, Belmalha Saadia, El Ghadraoui Lahsen

Ecological Engineering & Environmental Technology

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2024

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Vol. 25, iss. 10

60--74

EN

This study aimed to provide new data on the chemical composition and biological properties of Corrigiola. telephiifolia Pourr., demonstrating the influence of different extraction solvents and geographic locations on these properties. It presents novel findings on the phytochemical contents, antioxidant activity, and antimicrobial effects of various fractions of Corrigiola telephiifolia Pourr. roots collected from three distinct regions in Morocco. The obtained results reveal significant variations in TPC, TFC, and antioxidant activity depending on the extraction fraction and geographic origin. Notably, the fraction of raw extract exhibited the highest TPC values in all sampled sites (44.76 ± 0.05 in Agouray, 35.89±0.02 in Sefrou, and 14.99 ± 0.03 mg·GAE/g·dw in Azrou). The peak values of TFC were recorded in the fraction of raw extracts for all sampling sites (20.80 ± 0.01 in Agouray, 18.62 ± 0.01 in Sefrou, and 11.54 ± 0.01 mg QAE/g dw in Azrou). The highest values of FRAP were recorded in the fraction of raw extract of Agouray (2.00±0.00). Furthermore, the hexane fraction showed the highest DPPH and Chelating power with IC50 equal to 0.46 ± 0.02 mg·mL-1 and 0.10 ± 0.00 mg·mL-1, respectively. All fractions showed significant and variable antibacterial activities. The highest antimicrobial activity was recorded in in ethyl acetate fraction of the sample from Agouray against E. coli (MIC = 0.039). The highest anti-microbial effect was recorded also in ethyl acetate fraction against E. coli and P. aeruginosa, and raw fraction against B. subtilis and P. aeruginosa (MBC = 0.313 against both microorganisms). Agouray consistently demonstrated superior results across multiple parameters, indicating it as the best region for the highest phytochemical and bioactive properties. This study evaluated the chemical compounds and biological properties of Corrigiola. telephiifolia Pourr. and demonstrated the effect of the sampled area and solvent.

5

Myrtle Leaves (Myrtus communis L) and Olive Leaves (Olea europaea): Effect of Drying by Fluidization and Solar Methods on Key Bioactive Compounds Contents

Lekrati Mounia, Elfazazi Kaoutar, Laaraj Salah, Zerrouk Latifa, El Madani Saad

Ecological Engineering & Environmental Technology

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2024

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Vol. 25, iss. 2

210--217

EN

The present study aimed to evaluate the influence of drying by fluidization technology, compared to a traditional solar drying, on phenolic compounds of Moroccan Myrtus communis L. and Olea europaea L. species. Two main parameters of drying by fluidization (air speed (0.3-1 m/s for myrtle leaves and 1-3 m/s for olive leaves) and air temperature (40-60 °C for the two plants leaves)) were modified and controlled. Results showed that high loss values in total polyphenols (TPP) and flavonoids (TF) were observed at (60 °C; 0.3 m/s) for myrtle leaves and at (60 °C; 1 m/s) for olive leaves. However, these compounds were more stable at (40 °C; 1 m/s) for myrtle leaves and at (40 °C; 3 m/s) for olive leaves. Regarding the solar drying method, the results showed that, for myrtle leaves, high losses were observed in the TPP and TF contents, compared to the fluidization method for all processing parameters. For olive leaves, traditional solar drying gives products with phenolic contents similar to those obtained by fluidization drying, especially at (60 °C; 3 m/s). The findings indicated that employing the fluidization drying method might be a suitable approach for enhancing the conservation of bioactive compounds within myrtle and olive leaves.

6

Exploring the Bioactive Potential of Tamarix africana: Phytochemical Profiling, Antioxidant and Antibacterial Activities Assessment

Bihaoui Abdesslam, Idir Abderrazak, Nouri Ayoub, Talbi Hayat, Haddioui Abdelmajid, Hammada Soumaya

Ecological Engineering & Environmental Technology

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2024

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Vol. 25, iss. 6

188--199

EN

In Morocco, the Tamaricaceae family is represented by six species belonging to the Tamarix genus. including Tamarix africana which is utilized in traditional medicine to treat various ailments. This study aims to compare and evaluate the total polyphenol and flavonoid contents, as well as the antioxidant and antibacterial activity of Tamarix africana leaf and flower extracts obtained by Soxhlet extraction using five solvents of increasing polarity. The highest extraction yield was obtained with methanol for the leaves and flowers. Indeed, the results indicate that methanolic extracts contained the highest concentration of polyphenols and flavonoids for both organs (Polyphenols: 101.80 mg GAE/g DW in the leaf extract and 50.55 mg GAE/g DW in the flower extract. Flavonoids: 990.723 µg RE/g DW in the leaf extract and 630.84 µg RE/g DW in the flower extract). The results of antioxidant activity revealed that the aqueous extract of leaves and flowers of T. africana (IC50: 1.89 µg/mL and 3.175 µg/ mL respectively) had higher antioxidant activities than ascorbic acid. Concerning the antibacterial study, Bacillus subtilis showed resistance to the tested extracts. However, for the Citrobacter freundii strain, inhibition zones of 14 mm were recorded by the aqueous extract of flowers. On the other hand, the strong inhibition zones recorded against the Enterococcus faecalis strain, were 13 mm recorded by the leaves methanolic extract. Regarding the MIC, it is 6.25 mg/ml for the two strains. Concerning MBC, the results showed that the extracts are bacteriostatic in nature against Citrobacter freundii and Enterococcus faecalis. Thus, Tamarix africana seems to be a potential source of active molecules that could constitute a new alternative for medical and industrial use.

7

Comparative Analysis of Phenolic and Flavonoid content, Antioxidant, Antibacterial Activities, and Functional Groups of Chemicals from Hypericum perforatum L., and Papaver Rhoeas L. Flower Extracts

Afqir Hajar, Belmalha Saadia, Farihi Ayoub, Elbouzidi Amine, Bouhrim Mohamed, Elrherabi Amal, Boutagayout Abdellatif, Oubihi Asmae, Ouhssine Mohammed

Ecological Engineering & Environmental Technology

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2024

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Vol. 25, iss. 2

88--101

EN

In this study, the authors aimed to compare the phytochemical compounds (polyphenols and flavonoids), antioxidant activity, functional groups present in the compounds (FTIR), and anti-microbial effects, in the aqueous and methanol extracts obtained from flowers of commercial Hypericum perforatum and native Papaver Rhoeas from Morocco. P. Rhoeas L was collected from El Lhaj Kaddour near Meknes, while H. perforatum L was bought in a dried state from a Moroccan herbalist in the same city. Total polyphenols were evaluated using the Folin-Ciocalteu reagent, respectively. The antioxidant activity was assessed via DPPH and antimicrobial effects were tested against six bacteria (Gram– and Gram+). The aqueous and methanol extracts of P. Rhoeas had the highest TPC value (23.67 ± 0.94 mg GAE/g; 15.86 ± 0.65 mg GAE/g) compared to H. perforatum (15.26 ± 1.30 mg GAE/g; 5.50 ± 1.13 GAE/g). The aqueous extract of Papaver Rhoeas exhibited the highest TFC at 14.36 ± 0.49 mg QE/g, while the methanol extract of Hypericum perforatum had the highest 10.65 ± 0.49 mg QE/g in TFC. In contrast, the methanol and aqueous extracts of H. perforatum showed significant zones of inhibition against Staphylococcus aureus (9.5 ± 0.5 mm and 10.17 ± 0.29 mm) and Staphylococcus epidermidis (8.33 ± 0.58 mm and 9.33 ± 0.58 mm) respectively, with a minimum inhibitory concentration was estimated at 10 μL/ml. The FTIR analysis demonstrated that the extracts of both plants are rich in bioactive molecules with potential biological activities and a pharmaceutical industry perspective. Consequently, these Papaver Rhoeas and Hypericum perforatum extracts exhibit antioxidant and antibacterial activities.

8

Controlled drug delivery systems for improved efficacy and bioavailability of flavonoids

Gopikrishna A., Girigoswami A., Girigoswami K.

Journal of Achievements in Materials and Manufacturing Engineering

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2023

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Vol. 116, nr 2

49--60

EN

Purpose: In past decades, experiments have been done to find the properties of plant polyphenols and their protective role in various diseases. In the present study, a brief review has been done on flavonoids’ protective role in different diseases and controlled drug delivery systems that can be feasible for improving flavonoids’ bioavailability as well as their efficacy in the biological system. Design/methodology/approach: Keywords searched in PubMed, and Google Scholar are “Flavones and cardiovascular diseases, flavones and neurodegenerative diseases, isoflavones and neurodegenerative diseases, Flavonoids and ageing, Flavonoids and diseases, total flavonoid content in vegetables, total flavonoid content in fruits, controlled drug delivery system and flavonoids” and the significant recent articles are selected for writing this review. Findings: Flavonoids are active components present in plant products that have been found to exert several health benefits, especially in retarding the deleterious effects of CVD, cancer, ageing, diabetes, and neurodegenerative diseases. The different clinical studies have also supported the above notions, and in this commentary, we have highlighted some important findings in the field of flavonoid research. Even though it has various bioactive efficacy, most flavonoids have less bioavailability, requiring controlled drug delivery methods that can also improve flavonoids' bioavailability and stability. pH-, electro-, infrared radiation-, redox-responsive methods of controlled drug release systems are some of the valuable techniques for improving the rate of drug release and bioavailability at the targeted site. Research limitations/implications: Research is warranted in this field for improving and developing various materials that can be utilized in the formation of scaffolds/polymers that improves drug loading and controlled drug release properties at the targeted site. Originality/value: This review will help the readers to design new strategies in flavonoid research with the help of controlled drug release methods for increased bioavailability and rate of drug release/ controlled drug release.

9

Zastosowanie wybranych flawonoidów jako substancji aktywnych w medycynie regeneracyjnej

Młyniec Katarzyna, Träger Dominika, Niziołek Karina, Słota Dagmara

Inżynieria Materiałowa

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2023

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Vol. 44, nr 6

12--18

PL

Przedstawiono przegląd literatury na temat systemów dostarczania leków, charakterystyki poszczególnych flawonoidów i sposobów dostarczania ich do organizmu.

EN

A review of the literature on drug delivery systems, the characteristics of individual flavonoids as well as the ways of delivering them into the body.

10

Development and validation of ultra-high performance supercritical fluid chromatography method for quantitative determination of four target flavonoids components in citrus samples

Li Bao Qiong, Li Ze Ying, Li Xin Kang, Tan Lin Fan

Acta Chromatographica

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2023

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Vol. 35, no. 2

115--122

EN

A new method for the analysis of four target flavonoids in two kinds of citrus samples by ultra-high performance supercritical fluid chromatography (UHPSFC) method was developed. Main variables affecting the UHPSFC separation were optimized, and under the optimized conditions the four target compounds (tangeretin, nobiletin, hesperetin and naringenin) can be separated within 10 min. The UHPSFC method allowed the determination of the four target compounds in the diluted stock solutions with limit of detection (LOD) ranging from 1.08 to 2.28 μg mL⁻¹, and limit of quantification (LOQ) ranging from 1.45 to 4.52 μg mL⁻¹, respectively. The coefficients of determination (R2) of the calibration curves were higher than 0.9950. The recoveries of the four target compounds at three different concentrations were in the range of 82.4–117.6%. The validation results demonstrated that the proposed method is simple, accurate, time-saving and environment friendly, and it is applicable to a variety of complex samples such as medicine-food dual purpose herbs and functional foods.

11

Plant secondary metabolites as defenses : a review

Jusuf Herlina, Elveny Marischa, Azizova Feruza, Shichiyakh Rustem A., Kulikov Dmitriy, Al-Taee Muataz M., Atiyah Karrar K., Jalil Abduladheem T., Aravindhan Surendar

Journal of Water and Land Development

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2022

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no. 55

206--211

EN

Plant secondary metabolites have a variety of functions, including mediating relationships between organisms, responding to environmental challenges, and protecting plants against infections, pests, and herbivores. In a similar way, through controlling plant metabolism, plant microbiomes take part in many of the aforementioned processes indirectly or directly. Researchers have discovered that plants may affect their microbiome by secreting a variety of metabolites, and that the microbiome could likewise affect the metabolome of the host plant. Pesticides are agrochemicals that are employed to safeguard humans and plants from numerous illnesses in urban green zones, public health initiatives, and agricultural fields. The careless use of chemical pesticides is destroying our ecology. As a result, it is necessary to investigate environmentally benign alternatives to pathogen management, such as plant-based metabolites. According to literature, plant metabolites have been shown to have the ability to battle plant pathogens. Phenolics, flavonoids, and alkaloids are a few of the secondary metabolites of plants that have been covered in this study.

12

Characterization and identification of flavonoids from Bambusa chungii leaves extract by UPLC-ESI-Q-TOF-MS/MS

Yuan Ting, Guo Xue-Feng, Shao Si-Yue, An Rong-Miao, Wang Jin, Sun Jia

Acta Chromatographica

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2021

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Vol. 33, no. 3

281--294

EN

Bamboo leaves extract (BLE) has a variety of physiological functions such as antitumour, anti-inflammatory, antioxidant and blood fat reduction activities and the flavonoids of bamboo leaves are the major active constituents. To profile the flavonoids in the complex BLE, a rapid and sensitive analytical method based on ultra-high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UPLC-ESI-Q-TOF-MS/MS) was developed for the structural identification of the flavonoids in Bambusa chungii leaves extract using accurate mass measurements and characteristic fragmentation patterns. After separation on an Agilent SB-C18 Rapid Resolution High Definition (RRHD) column (2.1 mm × 150 mm, 1.8 μm) by gradient elution with 0.1% formic acid aqueous solution and acetonitrile as the mobile phase, the sample was analysed by ESI-QTOF-MS/MS in the negative mode. A total of 22 flavonoids were detected, and eight of these were identified by comparison with reference standards, while the other fourteen were tentatively identified according to their MS/MS data. The main fragmentation pathways of flavonoid C-glycosides (compounds 1, 5 and 10), flavonoid di-C,O-glycosides (compound 4), flavonoid di-C-glycosides (compound 7) and flavonoid C,O-di-glycosides (compounds 2 and 14) are shown in this article. This is the first report on the analysis of the flavonoids in the extract of B. chungii leaves. The present work demonstrates that UPLC-ESI-Q-TOF-MS/MS is an efficient technique for identifying multiple flavonoids of BLE.

13

Przetwarzanie odpadów z produkcji cebuli w żywność funkcjonalną

Piechowiak Tomasz, Józefczyk Radosław, Balawejder Maciej

Polish Journal for Sustainable Development

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2020

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T. 24, cz. 1

103--116

PL

W prezentowanej pracy przedstawiono przegląd dotychczasowego stanu prac badawczych związanych z wykorzystaniem łuski cebuli w produkcji żywności funkcjonalnej. Opisano dotychczasowe metody wyodrębniania związków bioaktywnych z łuski cebuli, wpływ antyoksydantów na właściwości funkcjonalne i prozdrowotne żywności oraz ich rolę w prewencji chorób cywilizacyjnych. Łuska cebuli jest odpadem powstającym podczas produkcji cebuli. Jak się okazuje, materiał ten, charakteryzuje się wysoką zawartością związków o charakterze antyoksydacyjnym, głównie flawonoidów (2-10 g/kg łupiny).

EN

In the paper a review of the current state of research works described in the literature and related to use of onion skins in the production of so-called functional food, is presented. The review was started by discussing the impact of antioxidants on the functional properties and health benefits of food and their role in the prevention of civilization diseases. Onion skin is waste generated during onion processing in the food industry and agriculture. As it turns out, this material has a high content of antioxidant compounds, mainly flavonoids (2-10 g / kg of onion skins). In the review current methods of the separation antioxidants, including those used in our own work, from the abovementioned skin, are discussed.

14

Natural Antioxidants as Multifunctional Additives for Polymeric Materials

Olejnik Olga, Masek Anna

Fibres & Textiles in Eastern Europe

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2020

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Vol. 28, no. 5 (143)

37--43

EN

The additional substances commonly used for polymeric materials mostly have a negative impact either on the natural environment or human health. Therefore, there is a need for replacing these compounds by more pro-ecological ones. In this paper, some natural antioxidants of plant origin, including xanthone, rutin hydrate, quercetin and a mixture of various polyphenols derived from green tea, were proposed as multifunctional as well as eco-friendly additional compounds for polymers. These substances were implemented by means of the impregnation process into ethylene-propylene copolymer (EPM) matrix. Some significant results of the research work are presented, including the mechanical properties and oxidation induction time (OIT) of the material tested. Moreover, ethylene-propylene copolymer (EPM) with antioxidants was investigated in terms of thermooxidative and UV ageing resistance. The impact of ageing processes on the material tested was analysed mainly by means of Fourier transform infrared spectroscopy (FT-IR) absorbance spectra and colour change assessment.

PL

Powszechnie wykorzystywane dodatki do materiałów polimerowych w większości posiadają negatywny wpływ zarówno na środowisko naturalne, jak i na zdrowie człowieka. Z tego względu istnieje duża potrzeba zastąpienia tych związków tymi bardziej proekologicznymi. W niniejszym artykule wybrane przeciwutleniacze pochodzenia roślinnego, takie jak: ksanton, rutyna (hydrat), kwercetyna oraz mieszanina różnych polifenoli otrzymana z zielnej herbaty zostały zaproponowane jako wielofunkcyjne, a za razem przyjazne środowisku naturalnemu dodatki do polimerów. Te substancje zostały wprowadzone do matrycy kopolimeru etylenowo-propylenowego (EPM) poprzez impregnację. Zaprezentowane zostały najważniejsze wyniki badań, takich jak pomiar właściwości mechanicznych czy czasu indukcji utleniania badanego materiału. Ponadto kopolimer etylenowo-propylenowy zawierający naturalne przeciwutleniacze został zbadany pod względem odporności na starzenie termo oksydacyjne i starzenie UV. Wpływ procesu starzenia na badany materiał został określony głównie na podstawie analizy widm absorpcyjnych uzyskanych za pomocą spektroskopii w podczerwieni (FT-IR) oraz poprzez ocenę zmiany barwy.

15

Kinetics of extraction of phenolic compounds and flavonoids from Carlina acaulis

Fedoryshyn Olha, Kniazieva Kateryna, Mylyanych Andriy, Petrinа Romana

ECONTECHMOD : An International Quarterly Journal on Economics of Technology and Modelling Processes

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2020

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Vol. 9, No 2

3--10

EN

The study of the kinetics of extraction of phenolic compounds and flavonoids from crushed roots of Carlina acaulis using 40% and 70% of water-ethanol mixture by infusion method is described in the article. The total value of the mass transfer coefficient and the value of the transfer coefficient through the cell wall, in the intercellular space and the volume of the extractant were determined. Particles of Carlina acaulis roots of different sizes (0.2, 0.3, 0.5 mm) were studied; different concentrations of ethyl alcohol were used - 40% and 70%; the ratio of raw materials: extractant was 1:10. The analytical dependence of the mass transfer coefficient k and the leaching coefficient A on the solid particle size d and the concentration of the extractant was obtained, which allows to predict the extraction process and to design equipment for the technological process in production. Kinetic equations of the process of extraction of phenolic compounds and flavonoids from Carlina acaulis roots by infusion method are derived. The obtained equations allow to determine the concentrations of phenolic compounds and flavonoids in the extracts at a given point in time with a particle size of the solid phase from 1 to 10 mm, as well as to determine the optimal diameter of the solid phase particles for maximum extraction of the target substance.

16

HPLC–MS/MS profiling of wild-growing scentless chamomile

Šibul Filip, Orčić Dejan, Berežni Sanja, Anačkov Goran, Mimica-Dukić Neda

Acta Chromatographica

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2020

|

Vol. 32, no. 2

86--94

EN

Scentless chamomile (Tripleurospermum inodorum = M. inodora) is a plant belonging to Anthemideae tribe of Asteraceae family, with phenotype similar to the common chamomile, a plant used in human consumption in the form of herbal tea infusion. In order to be able to understand possible health-promoting properties and adverse effects of the scentless chamomile's consumption, it is of essence to examine its chemical composition. The aim of the study was to perform phenolic profiling using high-performance liquid chromatography–tandem mass spectroscopy (HPLC–MS/MS), in comparison to the common chamomile. In the investigated extracts, qualitative and quantitative analyses enabled the identification of 66 compounds based on their retention times, mass (MS/MS) spectra, and analysis of their characteristic fragmentation patterns in MS/MS Product Ion Scan experiments. A new HPLC–MS/MS method for quantitation of common plant metabolites was hereby developed, enabling quantitation of 47 compounds. All examined M. inodora samples have relatively high combined phenolic and flavonoid contents (25.2–51.9 mg/g). Apigenin, apigenin-7-O-glucoside, luteolin, luteolin-7-O-glucoside, quinic acid, and 5-O-caffeoyl quinic acid were the compounds with highest concentration in both inodorous and common chamomile. The results obtained hereby represent the first and most detailed chemical profile of scentless chamomile so far.

17

Efficient separation of five flavonoids from Oxytropis falcata bunge by high-speed counter-current chromatography and their anticancer activity

Chen Chen, Liu Fengqin, Zhao Jingyang, Chen Tao, Li Yulin, Zhang Dejun

Acta Chromatographica

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2020

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Vol. 32, no. 3

189--193

EN

Oxytropis falcata Bunge, known as the “king of herbs” in Tibetan medicine, is used for treatment of hyperpyrexia, pain, wounds, inflammation, and anthrax. However, it is difficult to efficiently isolate compounds with high purity from O. falcata because of the complexity of traditional Tibetan medicines. In this study, the 80% ethanol elution fraction from extract by AB-8 macroporous resin column chromatography was demonstrated to have anticancer activity on human hepatoma SMMC-7721 cells in vitro. Then, a high-speed counter-current chromatography (HSCCC) method was successfully established for separation of compounds by using hexane–ethyl acetate–methanol–water (10:4:10:10, v/v/v/v) as the solvent system. Five flavonoids (7-hydroxyflavonone [1], 5,7-dihydroxy-4′-methoxy flavonol [2], 5,7-dihydroxyflavanone [3], 2′,4′-dihydroxychalcone [4], and 2′,4′-dihydroxydihydrochalcone [5]) were obtained in one-step separation with purities of 97.7%, 98.1%, 98.3%, 99.0%, and 98.3%, respectively. Finally, anticancer activities against the growth of SMMC-7721 cells of 5 flavonoids were confirmed. The IC50 values of the separated compounds were 213.45 μg/mL, 197.74 μg/mL, 375.16 μg/mL, 17.44 μg/mL, and 136.83 μg/mL in 24 h, respectively. The present study provided a basis for further development and utilization of this medicinal herb as a source of a new potential anticancer agent.

18

Characterization and identification of the major flavonoids in Phyllostachys edulis leaf extract by UPLC–QTOF–MS/MS

Shao S.-Y., Ting Y., Wang J., Sun J., Guo X.-F.

Acta Chromatographica

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2020

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Vol. 32, no. 4

228--237

EN

Phyllostachys edulis (PES), the most important bamboo species in China, is widely distributed in East Asia. Flavonoids, which are important bioactive natural compounds, often have similar structures, making their structural elucidation difficult. The aim of this study was to represent valuable, reliable mass spectral data for the identification of flavonoids in plant leaves. Ultra-performance liquid chromatography–quadrupole time-of-flight mass spectrometry (UPLC–Q-TOF-MS/MS) method was established for characterization and identification of the major flavonoids in PES leaf extract. A total of 13 flavonoids were simultaneously characterized, and their proposed characteristic product ions and fragmentation pathways were investigated. Thirteen compounds were separated on an Agilent Zorbax RRHD SB-C18 column (150 mm × 2.1 mm, 1.8 μm). On the basis of comparing with the 4 reference standards and the literature data, the other 9 flavonoids were identified by tandem mass spectrometry (MS/MS). Eight compounds (compounds 1, 4, 5, 8, 9, 10, 11, and 12) were found in PES leaves for the first time. An efficient UPLC–QTOF-MS/MS method was successfully applied for the structural identification of flavonoids in PES leaves. These results have practical applications for the rapid identification and structural characterization of these compounds in crude bioactive extracts or mixtures.

19

Impact of a Longwave UV-B Radiation on Soybean Plants Grown at Increased Nickel Concentration in Soil

Skórska Elżbieta, Matuszak-Slamani Renata, Romanowski Hubert, Szwarc Wiktor

Journal of Ecological Engineering

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2019

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Vol. 20, nr 5

135--141

EN

Plants of soybean (Glycine max L.) cv. Augusta grew in the soil with addition of Ni in the concentration of 80 (C-80) or 120 mg∙kg-1 dry mass (C-120) or without Ni (C-0), and they were subjected to UV-B (300 nm – 315 nm) dose of 1.8 kJ m-2 d-1 for 28 days. The addition of nickel into soil in the concentration of 120 mg kg-1 (C-120) caused a decrease of the plant height by 35% compared to the control (C-0), and in the presence of UV-B (U-120) – by 43%. The shoot fresh and dry mass in the C-80 and C-120 plants were lower by 33% and 52% than in the C-0. In the presence of Ni, the intensity of net photosynthesis decreased by 55%. UV-B caused an increase of flavonoid content by 25% compared to the control (C-0), and Ni induced a reduction in the content of these compounds from 20% to 40%.

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Farmakologiczna aktywność flawonoidów i aminoflawonów

Szeliga D., Korabik M., Ochocki J.

Wiadomości Chemiczne

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2018

|

[Z] 72, 7-8

563--583

EN

Aminoflavones belong to a group of flavonoids, compounds commonly found in nature. Their pharmacological and biochemical effects include cytotoxic, antioxidant and antitumor properties. The studies have shown that complexes of aminoflavons with metal ions can be potential drugs and seem to be promising in the treatment of ovarian cancer, breast cancer, lung adenocarcinoma and melanoma. In addition aminoflavones have a lower cytotoxic activity towards healthy cells than another compounds. In the view of their wide pharmacological and biological actions, they seem to have great therapeutic potential.