Road traffic is a common phenomenon in cities, and all urban road users suffer its negative effects. Therefore, the subject of this article is a comparative analysis of the exhaust emissions of toxic compounds and the operating parameters of two-wheeled vehicles measured under real operating conditions. The comparison applies to two drive cycles: in the first, the vehicle was travelling in standard city traffic (without using the bus lanes), while in the second, the vehicle travelled on bus lanes, whenever those were available. Such analysis made it possible to evaluate the ecological usefulness of bus lanes by assessing the exhaust emissions and the travel time of the test vehicle. For this purpose, an analysis of the exhaust emission values in time was performed for gaseous compounds (HC, CO, CO2, NOx) from a scooter equipped with a 125 cm3 displacement engine with a maximum power of 9 kW. The tests were carried out under real operating conditions in the city of Poznań. The route has been chosen so that it consisted of roads with a number of bus lanes. AXIONR/S+ mobile apparatus belonging to the PEMS (Portable Emissions Measurement Systems) group was used in the research. The device is one of the most modern measuring instruments, where the entire device is a suitcase weighing only 18 kg containing two analyzers for measuring the concentrations of both gaseous and solid exhaust components.