This paper presents research showing the problem occurring in the construction of a pipe organ, related to the placement of the organ pipes on the windchest. The close location of the organ pipes to each other influences the parameters of the sound generated by the pipes. It causes an intonation problem, namely the detuning of the organ pipes if they are located too close to each other on the windchest. The presented measurements show the influence of a distance between pipes of various types on basic sound parameters, such as frequency or volume level. The research carried out shows that in extreme cases the detuning reaches a temperate halftone. This has undoubtedly an impact on the tuning of organ pipes, especially in the case of a table organ or pipe organ built in a small space. In the future, the outcomes of the presented research can be applied in the windchest design.