Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 9

Liczba wyników na stronie
first rewind previous Strona / 1 next fast forward last
Wyniki wyszukiwania
Wyszukiwano:
w słowach kluczowych:  mouse
help Sortuj według:

help Ogranicz wyniki do:
first rewind previous Strona / 1 next fast forward last
PL
Paweł i Gaweł w jednym stali domu, Paweł na górze, a Gaweł na dole. W naszych domach Gawłem są szczury, a Pawłem myszy.
EN
The aim of the study was to determine the effect of the application of hyperbaric oxygen therapy on the course of an infection with group A type T-3 hemolytic β streptococcus. Experiments were carried out on Porton white mice and in vitro blood plates. General and local infections with streptococci were induced in animals. The infected animals were treated with hyperbaric oxygenation. The lethal effect of infection was significantly inhibited using hyperbaric oxygenation on the first and second day following the infection.
EN
An ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method was developed and validated for quantification of ligustroflavone, which was then applied in pharmacokinetics study in rat and tissue distribution in mouse. Twelve male Sprague Dawley rats were used for pharmacokinetics after intravenous (2 or 8 mg/kg) administration of ligustroflavone, six rats for each dose. Twenty-five mice were randomly divided into 5 groups (5 mice for each group, 1 group for each time point) and received 16 mg/kg ligustroflavone via intraperitoneal administration. The linear range of the calibration curve was over 2–2000 ng/mL for ligustroflavone in rat plasma and mouse tissues. The intra-day and inter-day precision expressed in % RSD were less than 14%, and the accuracy was between 88.5% and 108.4%.
4
Content available remote Pharmacokinetics of ebeiedinone in mouse blood by UPLC–MS/MS
EN
An ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method was established to determine ebeiedinone in mouse blood, and the pharmacokinetics of ebeiedinone after intravenous (0.5 mg/kg) and oral (2, 4, and 8 mg/kg) administration was studied. Twenty-four mice were randomly divided into 4 groups, 1 group was for intravenous administration (0.5 mg/kg), and other 3 groups were for oral administration (2, 4, and 8 mg/kg), with 6 rats in each group. Yubeinine was used as an internal standard. Multiple reaction monitoring (MRM) mode was used to quantitatively analyzed ebeiedinone m/z 414.4 → 91.1 and the internal standard m/z 430.4 → 412.3 in the electrospray ionization (ESI) positive interface. In the concentration range of 1–2000 ng/mL, the ebeiedinone in the mouse blood was linear (r2 > 0.995), and the lower limit of quantification was 1.0 ng/mL. In the mouse blood, the intra-day precision coefficient of variation (CV) was less than 15%, and the inter-day precision CV was less than 15%. The accuracy ranged from 85.4% to 114.6%, and the average recovery was higher than 61.3%. The matrix effect was between 87.0% and 106.5%. These data met the pharmacokinetic study requirements of ebeiedinone. The UPLC–MS/MS method was sensitive, rapid, and selective and was successfully applied to the pharmacokinetic study of ebeiedinone in mice. The absolute bioavailability of ebeiedinone was 30.6%.
EN
An ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method was established to determine the hapepunine in mouse blood, and the pharmacokinetics of hapepunine after intravenous (1.0 mg/kg) and intragastric (2.5, 5, and 10 mg/kg) administrations was studied. Delavinone was used as an internal standard. The UPLC ethylene bridged hybrid (BEH) C18 column was used for chromatographic separation. The mobile phase consisted of acetonitrile and 0.1% formic acid with a gradient elution flow rate of 0.4 mL/min. Multiple reaction monitoring (MRM) mode was used for quantitative analysis of hapepunine in electrospray ionization (ESI) positive interface. Proteins from mouse blood were removed by acetonitrile precipitation. The verification method was established in accordance with the US Food and Drug Administration (FDA) bioanalytical method validation guidelines. In the concentration range of 1–1000 ng/mL, the hapepunine in the mouse blood was linear (r2 > 0.995), and the lower limit of quantification was 1.0 ng/mL. In the mouse blood, the intra-day precision coefficient of variation (CV) was less than 12%, the inter-day precision CV was less than 14%. The accuracy ranged from 91.7% to 109.3%. The average recovery was higher than 76.7%, and the matrix effect was between 86.0% and 106.4%. The UPLC–MS/MS method was sensitive, rapid, and selective and was successfully applied to the pharmacokinetic study of hapepunine in mice. The absolute bioavailability of hapepunine was 22.0%.
EN
Bioimpedance indicating cell and tissue condition of the living things is of great importance in impedance spectrum analysis and other related researches, in which measurement of the skin impedance is a tricky problem due to the peculiarity of the stratum corneum. The aim of the study is to develop a method to elucidate the skin impedance in a large frequency range and to find out a biomarker to estimate it. In this article, we introduce a non-destructive method of using both surface and needle electrodes to investigate the electrical properties of the skin including stratum corneum of sixteen anesthetized C57BL/6 mice in vivo. A capacitance model was introduced to bridge the complex Debye's model and the practical three-element model. A new biomarker (tissue coefficient) was introduced to compare the whole skin with the viable skin. The contribution of the viable skin to the impedance of the whole skin can be ignored unless the concerned frequency is much higher than 10 kHz. The CG plot show direct link with the e-s plot, and the tissue coefficient shows significant difference between the whole skin and viable skin and reflects the alpha and beta dispersions. The results suggest that the method of using both surface and needle electrodes to investigate the impedance the whole skin including stratum corneum is practical, and the tissue coefficient is especially suitable for in vivo study, and has great potentials in estimation and discrimination of living tissues, cancer detection, bioelectrical impedance analysis (BIA) and other related fields.
7
Content available remote Pharmacokinetics of 8-O-acetylharpagide in mouse blood by UPLC–MS/MS
EN
8-O-Acetylharpagide is the main active component of the herb Ajuga decumbens, which possesses anti-tumor, anti-virus, and anti-inflammation properties. In this study, ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) was used to measure the concentration of 8-O-acetylharpagide in mouse blood, with subsequent investigation of the pharmacokinetics of the drug after intravenous or oral administration. Shanzhiside methyl ester was used as an internal standard, and the acetonitrile precipitation method was used to process the blood samples. Chromatographic separation was achieved using an ultra-performance liquid chromatography ethylene-bridged hybrid (UPLC BEH) column (2.1 mm × 50 mm, 1.7 μm) with a gradient methanol–water mobile phase (containing 0.1% formic acid). The flow rate was 0.4 mL/min, and the elution time was 5.0 min. 8-O-Acetylharpagide was quantitatively measured using electrospray ionization (ESI) tandem mass spectrometry in multiple reaction monitoring (MRM) mode with positive ionization. The result indicated that, within the range of 5–500 ng/mL, the linearity of 8-O-acetylharpagide in mouse blood was satisfactory (r > 0.995), and the lower limit of quantification (LLOQ) was 5 ng/mL. Intra-day precision relative standard deviation (RSD) of 8-O-acetylharpagide in blood was lower than 9%, and the inter-day precision RSD was lower than 13%. The accuracy range was between 94.3% and 107.1%, average recovery was higher than 91.3%, and the matrix effect was between 100.8% and 110.8%. This analytical method was sensitive and fast with good selectivity and was successfully applied to perform pharmacokinetic studies of 8-O-acetylharpagide in mice. The bioavailability of 8-O-acetylharpagide was 10.8%, and the analysis of the primary pharmacokinetic parameters after oral and intravenous administration indicated that 8-O-acetylharpagide had a significant first pass effect after oral administration.
8
Content available remote An artificial vision - based computer interface
EN
An application has been developed to assist in using software applications to individuals that have null or limited mobility of their upper limbs. The system uses a Web camera for movement patterns recognition of the user's face. The image analysis allows emulating the basic functions of the computer mouse. The face detection process is carried out through the implementation of an algorithm that uses a cascade sort key of Haar-Like type. The application was developed in C++ to be used on Windows XP platform. Tryout of the application has been performed showing excellent acceptation and short time training requirements.
PL
Opracowano oprogramowanie aplikacyjne pomagające korzystać z oprogramowania komputerowego osobom, które mają zerową lub ograniczoną ruchomość ich górnych kończyn. Taki system wykorzystuje kamerę internetową do rozpoznawania wzorców przemieszczeń ruchów twarzy użytkownika. Analiza obrazów pozwala na emulację podstawowych funkcji myszy komputerowej. Proces detekcji ruchów twarzy jest realizowany poprzez implementację algorytmu wykorzystującego kaskadowy klucz sortowania typu Haara. Oprogramowanie aplikacyjne zostało napisane w języku C++ i może być używane na platformie Windows XP. Zostały przeprowadzone próby oprogramowania, spotkały się one z doskonałym przyjęciem i akceptacją, gdyż używanie tego oprogramowania wymaga jedynie krótkiego treningu.
EN
Preliminary results of Magnetic Resonance Imaging (MRI) of cardiac function in mice in vivo, using the homebuilt gradient coils and specialized RF probehead are demonstrated. An unshielded gradient system with inner diameter of 60 mm was designed and constructed for the 4.7T/310 magnet with MARAN DRX console (Resonance Instruments). Dedicated probehead, constructed to fit the gradient system, consists of the RF birdcage coil and the animal handling system. ECG-triggered cine images of eight FVB (wild-type) and four transgenic micewith heart failure (Tg alfa q*44) were acquired at physiological temperature (37 graduate C) with a good quality of multislice images in different phases of the cardiac cycle, as well as a good contrast between myocardium and flowing blood. This technique allowed the calculation of the end-diastolic (EDV) and end-systolic (ESV) volumes of working heart that could be used to monitor the development of heart failure in vivo in Tg alfa q*44 mice.
first rewind previous Strona / 1 next fast forward last
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.