The main aim of the study was to experimentally determine the influence of low temperature on start-up and performance of an engine with high pressure fuel system; Common Rail system was used as the example here. The study included measurements of useful parameters of combustion and composition of exhaust gas, and determination of the influence of the fuel used on catalytic converter's performance. The studied engine was Renault G9T engine, fed with the following fuels: fuel base (diesel oil), aviation fuel, code NATO F-34, fuel blends of F-34 and rapeseed methyl esters of higher fatty acids, fuel blends of F-34 and anhydrous ethyl alcohol. The study showed that the parameters of Renault G9T engine with high pressure injection system fed with fuel blends of F-34 with biocomponents changed in comparison to those obtained with the use of the fuel base: diesel oil. The process of engine's ignition relies mainly on the proper functioning of engine starting system, fuel system and devices assisting the start-up.