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Nickel comb capacitors for real-time monitoring of cancer cell cultures

Kociubiński Andrzej, Zarzeczny Dawid

Przegląd Elektrotechniczny

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2020

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R. 96, nr 9

149--152

EN

The work is devoted to the technology of biocompatible substrates with nickel electrodes for in vitro impedance cell culture studies. The legitimacy of this subject was tested by conducting measurements using a system based on the Electric Cell-Substrate Impedance Sensor method. A device for cell bioimpedance testing, made in thin-film technology, has been described. Parameters and applications of the material used for construction, which is commonly used nickel, are discussed. The results of preliminary studies on melanoma cancer cells from the A375 cell line were presented, during which the already used measurement matrices were used. An analysis of the observed changes and obtained results was carried out.

PL

Praca poświęcona jest technologii biokompatybilnych podłoży z niklowymi elektrodami do badań impedancji hodowli komórek in vitro. Zasadność podjęcia tej tematyki przetestowano przeprowadzając pomiary przy użyciu systemu opartego na metodzie ECIS (ang. Electric CellSubstrate Impedance Sensor). Opisano przyrząd służący do badań bioimpedancji komórek, wykonany w technologii cienkowarstwowej. Omówiono parametry i zastosowania wykorzystanego do budowy urządzania materiału, którym jest powszechnie stosowany nikiel. Przedstawiono wyniki wstępnych badań nad komórkami nowotworowymi czerniaka z linii komórkowej A 375, do których użyto wykonane matryce pomiarowe. Dokonano analizy zaobserwowanych zmian i otrzymanych rezultatów.

2

Possible interactions between stent and electromagnetic field

Barz C., Petters M., Dorsz A., Syrek P.

Science, Technology and Innovation

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2018

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Vol. 3, no. 2

48--51

EN

Over the past years, an increase in the amount of the electromagnetic sources could be observed. Model presented in this article is limited to the impact of low frequency fields generated by the e.g. electrical power lines or magnetic coils in a bone fractures therapy. Particularly, the effect of the magnetic component of the electromagnetic field on stents will be evaluated. The conductivity of human tissues will be investigated. Yielded results will be used to simplify complicated, three-dimensional problem of the current distribution in stent branches, to one-dimensional one. The merits of the paper is proposing, implementing and using for analysis a numerical model of the stent in magnetic field. The impact of frequency and positioning of stent in the magnetic field will be investigated and current distributions found.

3

A biomechanical comparison between tissue stiffness meter and shore type 00 durometer using fresh human fetal membrane cadavers

Oflaz H.

Biocybernetics and Biomedical Engineering

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2016

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Vol. 36, no. 1

138--144

EN

A manual palpation is traditionally used on soft tissue stiffness evaluation in clinical practices. However, the palpation is a subjective technique, so quantitative tissue stiffness measurement would be a more reliable method on diagnosing disorders instead of a palpation in medicine. The purpose of this study was to emphasize a new medical device that was capable of measuring soft tissue stiffness. An in vitro investigation with a soft tissue stiffness meter (STSM) was presented and it is compared with a shore type 00 durometer in this study. Soft materials were needed for in vitro experiments to show feasibility of the STSM, so fetal membranes were decided to use on experiments. Five fetal membranes undergoing normal birth (NB) (35 samples, 105 measurements) and four fetal membranes undergoing pre-term birth (PRB) (20 samples, 60 measurements) were collected immediately after delivery. Samples were examined on custom designed tissue holder. Resu*lts of the STSM were in correlation with results of the durometer for NB and PRB (r2 = 0.995 and r2 = 0.996 respectively). Moreover, a tissue stiffness difference between NB and PRB was statistically significant by using STSM ( p ≤ 0.001), whereas it was not statistically significant by using durometer ( p = 0.360). In conclusion, newly produced device, STSM is more sensitive than durometer even for very small stiffness differences as between NB and PRB fetal membranes.