Wyniki wyszukiwania - BazTech

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The effect of nano-HA-based biomaterial on macrophage polarization and osteogenic differentiation in co-culture system in vitro

Kazimierczak Paulina, Przekora Agata

Engineering of Biomaterials

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2021

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Vol. 24, no. 163

14

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The study of the influence of micro-environmental signals on macrophage differentiation using a quantitative Petri net based model

Rżosińska K., Formanowicz D., Formanowicz P.

Archives of Control Sciences

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2017

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Vol. 27, no. 2

331--349

EN

The complexity of many biological processes, which, thanks to the development of many fields of science, becomes for us more and more obvious, makes these processes extremely interesting for further analysis. In this paper a quantitative model of the process of macrophage differentiation, which is essential for many phenomena occurring in the human body, is proposed and analyzed. The model is expressed in the language of Petri net theory on the basis of one of the three hypotheses concerning macrophage differentiation existing in the literature. The performed analysis allowed to find an importance of individual factors in the studied phenomenon.

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A novel approach for detection and delineation of cell nuclei using feature similarity index measure

John J., Nair M. S., Kumar P. R. A., Wilscy M.

Biocybernetics and Biomedical Engineering

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2016

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Vol. 36, no. 1

76--88

EN

Accurate image segmentation of cells and tissues is a challenging research area due to its vast applications in medical diagnosis. Seed detection is the basic and most essential step for the automated segmentation of microscopic images. This paper presents a robust, accurate and novel method for detecting cell nuclei which can be efficiently used for cell segmentation. We propose a template matching method using a feature similarity index measure (FSIM) for detecting nuclei positions in the image which can be further used as seeds for segmentation tasks. Initially, a Fuzzy C-Means clustering algorithm is applied on the image for separating the foreground region containing the individual and clustered nuclei regions. FSIM based template matching approach is then used for nuclei detection. FSIM makes use of low level texture features for comparisons and hence gives good results. The performance of the proposed method is evaluated on the gold standard dataset containing 36 images (_8000 nuclei) of tissue samples and also in vitro cultured cell images of Stromal Fibroblasts (5 images) and Human Macrophage cell line (4 images) using the statistical measures of Precision and Recall. The results are analyzed and compared with other state-of-the-art methods in the literature and software tools to prove its efficiency. Precision is found to be comparable and the Recall rate is found to exceed 92% for the gold standard dataset which shows considerable performance improvement over existing methods.

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Modulation of the growth of pulmonary tumour colonies in mice after single or fractionated low-level irradiations with X-rays

Nowosielska E. M., Cheda A., Wrembel-Wargocka J., Janiak M. K.

Nukleonika

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2008

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Vol. 53, suppl. 1

9-15

EN

A number of epidemiological and experimental data indicate that exposures to low doses of low-LET ionizing radiation may trigger the activity of natural anti-tumour immune mechanisms and inhibit tumour growth. Natural killer (NK) cells and activated macrophages play important roles in the anti-tumour defence of the host. In view of this, the aim of the present study was to correlate the tumour-inhibitory effect of low doses of X-rays with the activities of NK cells and macrophages. BALB/c mice were whole-body irradiated with single or fractionated doses of 0.1, 0.2, or 1.0 Gy X-rays and then intravenously injected with L1 sarcoma cells; 14 days later, tumour colonies on the lungs' surface were counted. Cytotoxic activities of NK cells and macrophages were estimated using the 51Cr-release and [3H]thymidineuptake assays, respectively. The anti-asialo GM1 antibody and carrageenan (CGN) were intraperitoneally injected to block the NK cell- and macrophage-mediated activities in vivo, respectively. Single and fractionated whole-body irradiation (WBI) of mice with 0.1 or 0.2 Gy X-rays led to reduction in the number of the pulmonary tumour colonies accompanied by the enhanced cytotoxic activities of both NK lymphocytes and macrophages. Treatment of mice with anti-asialo GM1 antibody or CGN abrogated the tumour-inhibitory effects of the low-level exposures to X-rays. The obtained data suggest that suppression of the development of pulmonary tumour colonies by single or fractionated irradiations of mice with the low doses of X-rays may result from stimulation of the natural anti-tumour defence reactions mediated by NK cells and/or cytotoxic macrophages.

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Stimulatory effects of single low-level irradiations with X-rays on functions of murine peritoneal macrophages

Cheda A., Wrembel-Wargocka J., Nowosielska E., Janiak M.

Nukleonika

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2005

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Vol. 50,suppl.2

13-16

EN

A number of epidemiological and experimental data indicate that exposures to low doses of low-LET ionising radiation may trigger the activity of natural anti-tumour immune mechanisms and inhibit tumour growth. In the present study, we assessed the cytotoxic activity and production of nitric oxide, superoxide anions, and tumour necrosis factor-alfa in peritoneal macrophages collected from BALB/c mice exposed to single whole-body irradiations with 0.1, 0.2, or 1.0 Gy X-rays. The results indicate that all the tested parameters were significantly up-regulated in macrophages obtained from mice exposed to 0.1 or 0.2 Gy X-rays but not in those collected from the sham-irradiated and 1.0 Gy-exposed animals.

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Different reactions of macrophages cultured with the samples from new and old polyethylene cups of the hip prosthesis

Czajkowska B., Otfinowski J., Ptak M., Pawelec A., Lekki J.

Inżynieria Biomateriałów

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2000

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R. 3, nr 11

8--10

EN

We estimated release of cytokines IL-6 and TNFα and tested viability of macrophages cultured with the samples of polyethylene obtained from new acetabular cup of the hip prostheses and from the cups retrieved 4-6 years after implantation. The macrophages cultured with old polyethylene showed decreased viability and released more IL-6 and TNFα.

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Luminol-Dependent Photoemission from Macrophages Stimulated by Cyclic Acetals of Salicylaldehyde

Maślińska-Solich J., Sowa M., Czuba Z.

Polish Journal of Chemistry

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1999

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Vol. 73, nr 8

1339-1343

EN

The synthesis and structure of several cyclic acetals of 2-hydroxybenzaldehyde (salicylaldehyde) and various 1,2- or 1,3-diols are described. These compounds were tested as the enhancers or inhibitors of luminol-dependent chemiluminescence of macrophages.

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In vitro study of chemically modified carbon fibres

Czajkowska B., Błażewicz M.

Inżynieria Biomateriałów

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1999

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R. 2, nr 5-6

25--30

EN

The objects of this study were three types of low-carbonised carbon fibres. The fibres differed in oxygen contents and in surface state resulting from chemical bonding. The behaviour of macrophages line J774 and peritoneal mouse macrophages in the presence of these fibres was studied. Viability, ability to proliferation and releasing level of IL-6 and NO were analysed. It has been shown that a/l examined fibres in- duce the formation of considerable high level of IL-6. Moreover, none of the analysed materials induced of NO forming. Macrophage viability varied depending on oxygen content on the carbon surface. Reaction of cells with modified surface carbon fibres brings about significant/y different cells response in vitro.