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PL
Celem pracy było określenie wpływu rodzaju techniki obróbki cieplnej, kulinarnej na właściwości fizykochemiczne, odżywcze i sensoryczne filetów z karpia (Cyprinus carpio) pokrytych aktywną powłoką furcelleranowo-żelatynową z dodatkiem ekstraktu z rozmarynu. Filety karpia, po naniesieniu powłoki, poddano: (i) pieczeniu w piecu konwekcyjno-parowym w środowisku pary wodnej, (ii) obróbce sous vide, (iii) pieczeniu w piekarniku w papilotach z folii aluminiowej oraz (iv) smażeniu w oleju rzepakowym w panierze z mąki ryżowej. Dokonano pomiarów parametrów barwy, składu chemicznego (zawartości wody, białka, tłuszczu i popiołu), cech tekstury oraz przeprowadzono ocenę organoleptyczną. Stwierdzono, że zastosowanie obróbki cieplnej zmodyfikowało barwę i skład chemiczny filetów w porownaniu do filetów surowych. Stwierdzono, że występują różnice we wpływie poszczególnych technik obróbki cieplnej na skład chemiczny, teksturę i barwę filetów. Obróbką kulinarną, pozwalającą na uzyskanie najbardziej pożądanych cech sensorycznych filetów karpia, było smażenie i może być ono rekomendowane jako metoda obróbki cieplnej filetów karpia pokrytych powłoką furcelleranżelatyna rybna z ekstraktem z rozmarynu.
EN
The study aimed to determine the effect of the type of culinary heat treatment technique on the physicochemical, nutritional and sensory properties of carp fillets (Cyprinus carpio) covered with an active furcellaran-gelatine coating with the addition of rosemary extract. After coating carp fillets, were subjected to: (i) heating in a convection-steam oven in a steam environment, (ii) sous vide processing, (iii) roasting in the oven in aluminium foil paper cases, and (iv) frying in rapeseed oil in batter from rice flour. Colour parameters, chemical composition (content of water, protein, fat and ash), texture characteristics were measured and organoleptic evaluation was carried out. It was found that the use of heat treatment modified the colour and chemical composition of fillets compared to raw fillets. It was found that there were differences in the effect of individual heat treatment techniques on the chemical composition, texture and colour of the fillets. The culinary treatment that allowed to obtain the most desirable sensory characteristics of carp fillets was frying and it can be recommended as a method of thermal treatment of carp fillets coated with furcelleran-fish gelatine with rosemary extract.
EN
Although the number of studies documenting the presence of Microplastics (MP) in fish is increasing, research studies focused on its detoxification are very limited. In this study, rainbow trout ( Oncorhyncus mykiss) were randomly divided into two groups after being fed with MPs (15% polypropylene [PP] +15% polyethylene [PE]) for 2 months. MP excretion without any application (PP+PE) in group I fish, and excretion of MPs with hydrogen-rich water (HRW) application (PP+PE+HRW) in group 2 were investigated under semi-static conditions for 21 days. This effectwas also compared by using positive and negative control groups (Control[no treatment, free PP, PE or/and HRW] and only HRW group). In thisdirection, the following were determined: PP+PE chronic toxicity in aquatic organisms, the toxicity mechanism and the effect of HRW as a possibletreatment method in blood tissue; with hematological indices ([RBC count [RBC], leukocyte count [WBC], hemoglobin value [Hb], hematocrit ratio [Hct], platelet count [PLT], hemoglobin count per erythrocyte [MCHC],mean hemoglobin amount per erythrocyte [MCH] and mean erythrocyte volume [MCV]) in other tissues (liver, gill and brain tissue) oxidative stress response (catalase [CAT]), glutathione peroxidase (GPx), glutathione reductase (GR), superoxide dismutase (SOD), glutathione (GSH), reactive oxygen products (ROS), malondialdehyde ([MDA] levels), DNA damage (8-OHdG: 8-Hydroxy-2-Deoxyguanosine), and the apoptosis (caspase 3) levels were investigated. In addition, acetylcholinesterase enzyme (AChE) activity, which is important in neurotoxicity pathways in the brain, wasdetermined. The presence of plastics (PP/PE) in target tissues (muscle, liver,gill and gastrointestinal tract) was also obtained.The results showed that PP+PE caused toxicity in all three tissues. MPs showed an inhibiting effect on antioxidant enzyme activities and an inductive effect on MDA, ROS, 8-OHdG, and caspase 3 levels. HRW showed a mitigating effect on MP-mediated toxicity in O. mykiss brain, blood, gill, and liver by controlling the ROS/ GSH/MDA pathway. HRW can be suggested as a cost-effective and eco-friendly curative for the protection of fish from the oxidative damages produced by the ingestion of microplastics.
EN
It is an acknowledged theory that a faster growth rate, determined by various environmental drivers, may boost the survival of larval and juvenile fish. In this study we examined the differences in the growth rate of larval and juvenile cod (age 2 – 136 d; SL: 4.1 – 39.2 mm) between the years 2006 and 2014, sub-areas of the Baltic Sea (Bornholm Basin, BB; Słupsk Furrow, SF; Gdansk Basin, GB), and seasons (spring and summer). The average growth rate for all specimens was 0.25 mm/d, with significantly lower values between 2012 and 2014 than between 2006 and 2011. A reduction in zooplankton biomass, especially the large zooplankton fraction, was observed after the 2006–2008 period, which was related to the prevailing temperature conditions in the surface layers. The reduction in zooplankton biomass was accompanied by changes in the structure of zooplankton: less T. longicornis, C. hamatus, and Pseudocalanus spp., and more Acartia spp. The results suggest that the inter-annual differences observed in zooplankton biomass and structure are likely responsible for the observed reduction in the growth rate of cod larvae between 2012 and 2014 compared to the years between 2006 and 2011. The growth rate reduction could be one of the reasons for the decline in the recruitment of eastern Baltic cod between the years 2013 and 2015, after the high recruitment years of the period 2011-2012.
EN
Plastic is one of the main pollutant sources that are difficult to decompose and then carried into the ocean and fragmented into smaller parts (microplastics) due to UV radiation and water currents. Their small size means that microplastics are often ingested by aquatic organisms, such as fish. This research aimed to determine the presence, abundance, and types of microplastics in the digestive tract of four dominant fishes landed at Lengkong Fish Auction Point, Cilacap, Central Java, i.e. threadfin (Eleutheronema tetradactylum), mackerel (Rastrelliger sp.), threadfin bream (Nemipterus japonicus), and hairtail (Trichiurus lepturus). We found microplastics in the digestive tract of four selected fishes with a frequency of occurrence of 100%. The concentration of microplastics in fish digestive tracts is relatively high, with a value range of 12 ±2.86 to 28.33 ±8.11 particles∙ind.-1. Microplastics were found in films, fibres, fragments, and granule shape types with various colours: brown, purple, blue, black, green, transparent, and yellow. The polymers found were polystyrene (PS), nylon, acrylonitrile butadiene styrene (ABS), polyurethane (PU), polypropylene (PP), high-density polyethylene (HDPE), and low-density polyethylene (LDPE). The present study provides baseline data for microplastics contamination in commercial fish species landed at Lengkong Fish Auction Point, Cilacap, Central Java, Indonesia. The fact that we discovered PU, the most harmful polymer, piques our attention.
EN
Watercourses serve the needs of agriculture in certain sections and are subject to strong anthropopressure. When agriculture prevails in the catchment, it often has abundant vegetation, macrophytes in particular, and the bottom is covered by a substantial layer of silt. In order to ensure patency of flow and access to water, small watercourses are very often subject to river maintenance works. The aim of the study was to assess the impact of 3 types of works on the diversity of fish species and density of fish in the 1 and 2 year after the works at 21 sites located on 8 rivers in the European Central Plains Ecoregion. As a result of the works, especially in the 1 year after the works, significant changes were found in hydrochemical and hydrological parameters, especially with the increase in velocity and mean depth in watercourses. Additionally, habitat change contributed to differences in the composition of the ichthyofaunal. After 1 year, the diversity of fish species and fish population density increased or remained unchanged after dredging and removal of plants from the river channel, but they decreased after the removal of macrophytes from river banks. After year 2, a decrease in the diversity of fish species and density was observed, associated with re-infestation and siltation of the watercourses. Particularly in the case of dredging and removal of macrophytes from the river bank, maintenance works should not be done too frequently, because some species of natural value disappear.
PL
Ryby oraz produkty nieprzetworzone z ryb należą do produktów szybko psujących się. Jest to wynikiem przemian zachodzących w surowcach rybnych nie poddanych konserwacji po złowieniu, jak również złego przechowywania tych surowców. Z tego względu coraz więcej uwagi poświęca się badaniom nad wprowadzeniem nowoczesnych metod konserwacji oraz pakowania żywności. Jadalne powłoki bądź folie stanowią interesującą alternatywę dla tradycyjnych metod zwiększenia trwałości pożywienia, poprzez ochronne działanie przed czynnikami środowiska zewnętrznego, jak i wewnętrznego. Dodatkowo wprowadzenie takiej metody ochrony dla produktów rybnych będzie korzystnie wpływało na ekologię, dzięki zmniejszonej produkcji syntetycznych opakowań.
EN
Fish and unprocessed fish products are perishable products. This is the result of changes in raw fish that are not preserved after being caught, as well as poor storage of this raw material. For this reason, more and more attention is paid to research into the introduction of modern methods of food preservation and packaging. Edible coatings or films are an interesting alternative to traditional methods of extending the shelf life of food by protecting against external and internal environmental factors. In addition, the introduction of such a method of protec-tion for fish products will have a positive effect on ecology, thanks to the reduced production of synthetic packaging.
EN
In this study, neurotoxic responses to exposure to chlorpyrifos (CPF) at different doses (55 and 110 μg l-1) and at different time intervals (24 and 96 h) were investigated in Siraz fish (Capoeta umbla) using 8-hydroxy 2-deoxyguanosine (8-OHdG) activity, caspase-3, acetylcholinesterase (AChE) and oxidative stress parameters [malondialdehyde (MDA), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione reductase (GR)]. In this study, the LC50 value of CPF was deterined for the first time for C. umbla and calculated as 440 μg l-1. In this study, 12.5% (55 μg l-1) and 25% (110 μg l-1) of the LC50 value were used. The obtained data indicate a significant increase in the MDA level and inhibition of antioxidant enzymes in the brain (p < 0.05). Considering DNA damage and the apoptotic process, no significant changes were found in 8-OHdG and caspase-3 activity at both doses exposed for 24 h, but a significant increase was detected in both markers at 96 hours compared to the control group (p < 0.05). In the case of AChE activity, which is one of the neurotoxic markers in the brain, while inhibition was determined only at the high concentration (110 μg l-1) at the end of 24 hours, a decrease in enzyme activity was observed at the end of 96 hours in both concentration groups. In the light of all these results, we can say that CPF showed inhibitory effects on enzyme activity and inducing effects on MDA, caspase-3 and 8-OHdG levels. Based on these results, it can be concluded that CPF contributes to oxidative stress in fish and may have neurotoxic effects.
EN
Pharmaceuticals which are widely used in aquatic can easily migrate into the environment and aquatic animals, and can increase the risk of drug resistance and allergic symptoms if consumed by humans. In order to achieve high-throughput analysis of pharmaceuticals with different physical and chemical properties from complex matrices, we developed a new method for various types pharmaceuticals in fish and shrimp tissue. Series solid-phase extraction (s-SPE) with different adsorbents was selected for extracting and purifying analytes with different paddings. s-SPE were combined with ultra performance liquid chromatography triple quadruple tandem mass spectrometry (UPLC-MS/MS) for the detection of 30 pharmaceuticals antibiotics in fish samples. This method was stabilized and reliable to determinate the pharmaceuticals in fish and shrimp samples. As the method combined multiple Chinese national standards method, it could be easily treat the multi-pharmaceuticals from the fish and shrimp samples once time. It provided for both quantitative and qualitative methods and they could be applied to single- or multi-residue methods.
EN
The research analyzed seasonal changes of the oxygen regime and related indicators on the example of water objects of the Ukrainian Polesie Region. The region shows different directions of economic use. Zebrafish (Danio rerio Hamilton–Buchanan) and the Prussian carp (Carassius auratus gibelio Bloch) were used as test objects to investigate survival responses. Dissolved oxygen (DO) concentration in water, pH values and temperatures were determined by standard methods. Based on research results, the main problems were determined pertaining to the oxygen regime of investigated waters, i.e. the increase in temperature and toxicity of the aquatic environment in the summer. A rather dangerous decrease in DO concentration, almost up to the levels of maximum allowable concentration (MAC) (4.10 mg∙dm–3 in group E1 and 6.07 mg∙dm–3 in group E2), was observed in August and it was typical for the reservoirs with a slow water movement. Flowing river waters (group E3) were eliminated due to their better aeration compared to other groups. The correlation analysis based on the presented data revealed a high and average degree of probable correlation between the DO concentration and water temperature, as well as an average degree of correlation with general toxicity determined on sensitive species of D. rerio, and in group E1 on the persistent species C. auratus gibelio as well. The interrelations and equations of the rectilinear regression can be used to predict the oxygen regime of the waters investigated and other surface waters having similar problems.
EN
The pollution of rivers from human activities such as housing, markets, workshops, transportation, land cultivation, and industry has become an issue. The increasing contribution of heavy metals to pollution in rivers has a direct effect on the metal bioaccumulation in fish. Therefore, this study aimed to examine the concentrations of Cd, Cu, and Pb in the waters, sediment, and fish harvested from the Krueng Sabee River, Aceh Province, Indonesia. Furthermore, the water, sediment, and fish samples were collected from six locations representing the upstream and downstream regions of the river. Cd, Cu, and Pb were analyzed using Atomic Absorption Spectrophotometry (AAS). The results showed that Cd, Cu, and Pb were not detected in the water, while the concentration of Cd in the sediments ranges between 0.0544 to 0.2683 mg kg-1, Cu ranges between 4.4149 to 14.8160 mg kg-1, and Pb ranges between 0.9186–15.4954 mg kg-1. Therefore, Cd, Cu, and Pb in the water and sediment were below the quality standard, but these heavy metals in the fish sample met the threshold limit. The highest Cd concentration was reported in Tor soro (5.5591 mg kg-1), and the highest concentration of Cu was reported in Mugil cephalus (6.7021 mg kg-1), while the higher Pb concentration was reported in Cyclocheilichthys Apogon (0.0279 mg kg-1).
EN
The investigation within the framework of this study was carried out on three marine species fished off in Moroccan coasts, namely: a fish, the sole (Solea Vulgaris), a cephalopod, the cuttlefish (Sepia Officinalis) and a crustacean, the pink shrimp (Parapenaeus Longirostris). The aims of this study were assessing the level of concentration in these three marine species, of three metallic trace elements: lead (Pb), cadmium (Cd) and mercury (Hg) and giving a reflection on the way in which Moroccan deep-sea fishing professionals can join a food safety management system to ensure the safety of their product and promote their competitiveness. Sampling was carried out at the port of Agadir city after landing of the deep-sea fishing boats which operate beyond 10 nautical miles, offshoring the seabed of Agadir for pink shrimp (Parapenaeus Longirostris), and in Dakhla city offshore for sole (Solea Vulgaris) and cuttlefish (Sepia Officinalis). Trace metal analysis was performed on individual muscles belonging to the three species. The results of the conducted investigations show that the Pb content varies between 0.01 ppm and 0.27 ppm in Solea Vulgaris; between 0.015 ppm and 0.16 ppm in Sepia Officinalis and between 0.022 ppm and 0.38 ppm for Parapenaeus Longirostris. For Cd, the concentrations vary between 0.0047 ppm and 0.050 ppm in Solea Vulgaris; between 0.0025 ppm and 0.254 ppm in Sepia Officinalis and between 0.0065 ppm and 0.11 ppm in Parapenaeus Longirostris. Concerning Hg, the contents vary between 0.0006 ppm and 0.075 ppm in Solea Vulgaris; between 0.0008 ppm and 0.05 ppm in Sepia Officinalis and between 0.0016 ppm and 0.09 ppm in Parapenaeus Longirostris. It appears from this study that the metal contents detected at the level of the three species of seafood investigated are below the regulatory thresholds. These results can be considered as the first basis for validating the control measure linked to the absence of metallic contamination of the three species in the sites studied.
PL
Przetwórstwo rybne jest ważnym elementem sektora produkcji żywności UE. W artykule dokonano przeglądu najważniejszych dla przetwórstwa ryb i owoców morza kategorii surowców, określając ich aktualną dostępność dla przemysłu. Ze względu na malejące dostawy najważniejszych dla przetwórstwa gatunków (dorsz, tuńczyk, łosoś, krewetki) ich ceny w ostatnich latach wzrosły, a efektem jest ograniczenie popytu na wytwarzane z nich produkty i mniej dynamiczny niż poprzednio rozwój przemysłu.
EN
The fish processing industry is a significant EU food production sector. The article reviews the most vital resources for the fish and seafood processing industry at the same time evaluating their current accessibility for the industry. Due to the decreasing supply of the vital for the industry species [cod, tuna, salmon, shrimp] their prices in the recent years have increased resulting in the lowered demand on the manufactured product which in turn leads to less dynamic development of industry than before.
PL
W diecie przeciętnego konsumenta obserwuje się przewagę wielonienasyconych kwasów tłuszczowych (PUFA) omega-6 (np. linolowego, LA), co prowadzi do niedoborów kwasów omega-3 (np. α-linolenowego, ALA; dokozaheksaenowego, DHA; eikozapentaenowego, EPA). Powszechnie stosowane oleje roślinne, tj. słonecznikowy, rzepakowy czy sojowy, zawierają dużo PUFA omega-6. Ze względu na szereg korzyści zdrowotnych zaleca się wzbogacanie diety w źródła PUFA omega-3. Kwas ALA można dostarczyć w postaci np. oleju lnianego, nasion chia, orzechów czy zielonych warzyw. Znaczące ilości DHA i EPA występują w rybach morskich. Alternatywne źrodła PUFA omega-3 to mikroalgi, rośliny transgeniczne bądź owady jadalne, których konsumpcja nie jest zbyt duża, dlatego praktykuje się wzbogacanie w PUFA omega-3 żywności tradycyjnej, np. produktów mlecznych, wypieków, tłuszczów do pieczenia i smarowania pieczywa, sosów czy napojów. Poza ich bezpośrednim dodatkiem do produktów stosuje się tzw. biofortyfikację, czyli wprowadzanie NNKT omega-3 do pasz trzody chlewnej (mięso), krów mlecznych (mleko) czy kur niosek (jaja). Produkty z dużą zawartością PUFA omega-3, po spełnieniu określonych wymagań, mogą być zaliczane do żywności funkcjonalnej.
EN
In the diet of the average consumer, the predominance of omega-6 (e.g. linoleic, LA) polyunsaturated fatty acids (PUFA) is observed, which leads to deficiencies of omega-3 (e.g., α-linolenic, ALA; docosahexaenoic, DHA; eicosapentaenoic, EPA). Commonly used vegetable oils, such as sunflower, rapeseed and soybean oils, contain a lot of PUFA omega-6. Due to a number of health benefits, it is recommended to enrich the diet with PUFA omega-3 sources. ALA acid can be supplied by consumption e.g. linseed oil, chia seeds, nuts or green vegetables. Significant amounts PUFA omega-3 are microalgae, transgenic plants or edible insects, the consumption of which is not very high, therefore nowadays traditional foods are enriched with PUFA omega-3, e.g. dairy products, baked goods, baking fats and spreads, sauces, and beverages. Currently also so-called biofortification is being used. It is the addition of omega-3 EFAs to feed of pigs (meat), dairy cows (milk) or laying hens (eggs). Products with a high content of PUFA omega-3, after meeting certain requirements, can be classified as functional food.
RU
Predlagaetsâ rešenie differencial’noogo uravneniâ dviženiâ ryby na vibroploskosti čislennym metodom, kotoryj daet vozmožnost’ ocenit’ putem virtual’nogo eksperimenta vliânie frikcionnyh harakteristik ryby na dinamiku processa. Ustanovlen naibolee racional’nyj režim vibroperemeŝeniâ, razrabotany rekomendacii po podboru frikcionnoj pary „ryba-material tehnologičeskoj poverhnosti” s koefficientom treniâ v oblasti malyh značenij.
PL
W artykule zaproponowano rozwiązanie równania różniczkowego ruchu ryb na płaszczyźnie wibracyjnej metodą numeryczną, co umożliwia ocenę za pomocą wirtualnego eksperymentu wpływu cech tarcia ryb na dynamikę procesu. Ustalono najbardziej racjonalny tryb przemieszczania drgań, opracowano zalecenia dotyczące wyboru pary ciernej „materiał rybny -powierzchnia technologiczna” o współczynniku tarcia w obszarze małych wartości.
EN
Hashimoto disease is one of the most commonly recognized autoimmune and endocrine diseases. The development and the course of the ailment depends on a variety of factors, nutritional among other things. Fish and fish-based products contain nutrients regulating the homeostasis of thyroid hormones and the immune system functions. The paper provides up-to-date knowledge on the mechanisms of effects exerted by nutrients contained in fish on the thyroid functions and thyroid-released hormones, and on the course of inflammation in this gland. Familiarity with these issues is necessary for a proper planning of nutrition in Hashimoto-affected patients.
PL
Choroba Hashimoto jest jedną z najczęściej rozpoznawanych chorób autoimmunologicznych i endokrynnych. Rozwój i przebieg schorzenia zależy od wielu czynników, w tym żywieniowych. Ryby i ich przetwory są produktami zawierającymi składniki regulujące homeostazę hormonów tarczycy i funkcjonowanie układu odpornościowego. W artykule scharakteryzowano aktualną wiedzę na temat mechanizmów wpływu składników zawartych w rybach na działanie tarczycy i wydzielanych przez nią hormonów oraz przebieg procesu zapalnego w gruczole. Ich znajomość jest niezbędna do prawidłowego planowania żywienia dla osób z chorobą Hashimoto.
EN
We have determined how Crustacea, zebra mussels (Dreissena polymorpha), fish and nutrients affect the trophic state in mesocosms filled with water collected from the pelagic zone of a eutrophic lake. We hypothesized that the pool of nutrients would increase both directly due to the input of phosphorus and nitrogen and/or indirectly due to the introduction of animal biomass. We used trophic state indices based on the abundance and species structure of rotifers to assess changes in the trophic state in mesocosms. The role of small detritophages in rotifer communities increased as a result of treatments. Our results clearly demonstrated that D. polymorpha was able to reduce the trophic status of mesocosm waters as indicated by reduced rotifer trophic state indices regardless of interactions with other treatment factors. Contrary to our expectations, neither the nutrients added at the beginning of the experiment nor the introduction of crustaceans or fish affected the rotifer trophic indices.
EN
Epizoic diatom communities were studied on four turtle species (Chelonia mydas L., Eretmochelys imbricata L., Lepidochelys olivacea Eschscholtz and Dermochelys coriacea Vandelli) from the Eastern Caribbean, the Equatorial West Atlantic and the South Pacific. In the present study, we focused on one taxon of Navicula Bory de Saint-Vincent, described here as a new taxon, which was found on seven individuals of Dermochelys coriacea and one individual of Lepidochelys olivacea in French Guiana. The new Navicula taxon was found neither on Chelonia mydas (83 specimens examined) nor Eretmochelys imbricata (13 specimens examined). Furthermore, the new taxon appears to have a restricted biogeography, as so far it has only been reported from French Guiana. A species of Navicula is reported for the first time as a supposedly exclusive epizoic taxon. Navicula dermochelycola sp. nov. is characterized in detail; its ultrastructure is described and compared with other members of Navicula.
EN
In this study, muscle samples collected from Planiliza subviridis (Valenciennes, 1836) and Ellochelon vaigiensis (Quoy & Gaimard, 1825) caught on the Balochistan coast (Damb Harbor) between January and December 2015 (during the northeast monsoon, post-monsoon and pre-monsoon seasons, and the southwest monsoon) were analyzed to determine concentrations of heavy metals: iron, zinc, copper, manganese, cadmium and lead by an atomic absorption spectrophotometer, expressed per unit of dry weight of each sample. The average measured level of Fe, Zn, Cu, Mn, Cd and Pb for P. subviridis was 26.70 ± 11.49 μg g-1, 13.82 ± 4.56 μg g-1, 1.66 ± 0.84 μg g-1, 0.24 ± 0.10 μg g-1, 0.06 ± 0.07 μg g-1 and 0.17 ± 0.14 μg g-1, respectively. The average level of the same metals for E. vaigiensis was 29.26 ± 10.18 μg g-1, 18.85 ± 6.28 μg g-1, 2.18 ± 1.01 μg g-1, 0.32 ± 0.14 μg g-1, 0.25 ± 0.11 μg g-1 and 0.30 ± 0.12 μg g-1, respectively. The highest Pb accumulation (0.56 μg g-1 and 0.61 μg g-1) detected in P. subviridis and E. vaigiensis is above the limit value (0.50 μg g-1) reported by FAO, hence the accumulation of Pb in these two fish species should be monitored in the future.
EN
Increasing anthropopressure affects natural ecosystems and may express itself in regional or spot contamination of water and soil environment. The quality of ground and surface waters depends to a large extent on drainage area management, where biogenic substances (i.e. nitrogen, phosphorus and potassium) are transported with surface run-offs to the environment. The article discusses results of studies on the drainage area of Lake Czolnowskie (Zachodniopomorskie Province). Studies covered physical and chemical assessment of soil conditions and water quality; additionally, fish was caught using two independent methods - gillnets and power generator (pursuant to CEN EN 14011 2003 and PN-EN 14011 2006). Results obtained confirm regular run-off of biogenic materials from the surface of farmed drainage area to Lake Czolnowskie. During the period of studies, the reservoir was exposed to i.e. reduction of water oxygen (3.56 mg/dm3) and permanently elevated level of general phosphorus (0.15-0.27 mg/dm3) and ammonium acid (0.16-0.27 mg/dm3), which degraded the quality of life for ichthyofauna inhabiting the lake.
EN
This study aimed to develop a chromatographic method to quantitatively determine phenol in fish tissues. This method involves solvent extraction of acidified samples, followed by derivatization to phenyl acetate and analysis with gas chromatography coupled with mass spectrometry (GC–MS). Phenol in a representative tissue sample (belly, gill, or renal tubules), which was homogenized with 2 N sulfuric acid, was extracted with ethyl acetate and derivatized to phenyl acetate using acetic anhydride and K2CO3 in water. An n-butyl acetate extract was injected into the GC–MS. The linearity (r2) of the calibration curve was greater than 0.996. The analytical repeatability, which is expressed as the relative standard deviation, was less than 6.14%, and the recovery was greater than 96.3%. The method detection limit and the limit of quantitation were 8.0 μg/kg and 26 μg/kg, respectively. The proposed method is also applicable to the analysis of other biological tissues for phenol and its analogs, such as pentachlorophenol.
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