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Simulated Activation Patterns of Biological Neurons Cultured onto a Multi-Electrode Array Based on a Modified Izhikevich's Model

Saito J. H., Mari J.-F., Pedrino E., Destro-Filho J. B., Nicoletti M. C.

Fundamenta Informaticae

2013

Vol. 124, nr 1/2

111--132

Recently we have witnessed research efforts into developing real-time hybrid systems implementing interactions between computational models and live tissues, in an attempt to learn more about the functioning of biological neural networks. A fundamental role in the development of such systems is played by Multi-Electrode Array (MEA). In vitro cultures of neurons on MEAs, however, have some drawbacks such as: needing a rigorous adherence to sterile techniques, careful choice and replenishment of media and maintenance of pH, temperature, and osmolarity. An alternative way to study and investigate live tissues which partially circumvent some of the problems with in vitro cultures is by simulating them. This paper describes the proposal of Sim-MEA, a system for modeling and simulating neuron's communications in a MEA-based in vitro culture. Sim-MEA implements a modified Izhikevich model that takes into account both: distances between neurons and distances between microelectrodes and neurons. The system also provides ways of simulating microelectrodes and their recorded signals as well as recovering experimental MEA culture data, from their images. The soundness of the Sim-MEA simulation procedure was empirically evaluated using data from an experimental in vitro cultured hippocampal neurons of Wistar rat embryos. Results from simulations, compared to those of the in vitro experiment, are presented and discussed. The paper also describes a few experiments (varying several parameter values) to illustrate and detail the approach.

Load-carrying capacity of mine road supports as a factor protecting mine work environment against rock bursts

Brodny J.

Górnictwo i Geologia

2009

T. 4, z. 2a

5-13

Rock bursts constitute one of the main natural hazards of work environment in the Polish mining industry. Their consequences may be tragic both for people and for machines and other equipment present in their influence zone. This contribution presents results of research work aimed at determining if and how load-carrying capacity of mine road supports influences a possibility of occurrence and intensity of rock bursts. The research work was performed for coals in triaxial stress conditions analogues to the in-situ conditions existing in rock mass where rock bursts come into being. Based on an analysis of obtained results it is justified to state that load bearing capacity of mine road supports has an essential influence on possibility of occurrence and intensity of rock bursts. The pressure exerted by mine supports on surrounding rock mass influences the pressure state at which rock bursts come into being. The analyses were accomplished based on results obtained during stand tests.

Zjawisko tąpań to jedno z głównych naturalnych zagrożeń środowiska pracy w górnictwie węglowym w Polsce. Skutki tąpań bywają bardzo groźne zarówno dla ludzi, jak i dla maszyn i urządzeń znajdujących się w strefie ich wystąpienia. W artykule zostały przedstawione wyniki badań mających na celu określenie, czy i jaki wpływ na możliwość wystąpienia tąpnięcia i jego intensywność ma nośność obudowy chodnikowej. Badania zostały przeprowadzone dla węgli poddanych trójkierunkowemu stanowi naprężenia analogicznemu do stanu rzeczywistego w górotworze, w którym dochodzi do tąpnięć. Na podstawie analizy uzyskanych wyników należy stwierdzić, że nośność obudowy chodnikowej ma istotny wpływ na możliwość wystąpienia tąpnięcia oraz na jego intensywność. Nośności obudowy górniczej istotnie wpływa na stan naprężenia w górotworze, przy którym dochodzi do tąpań. Analizy zostały dokonane na podstawie wyników badań stanowiskowych.